Summary
Potato plantlets derived from in vitro propagation of three cultivars known for their field resistance (Cruza 148 and BR-63.65) or susceptibility (Désirée) to Pseudomonas solanacearum E.F. Smith were artificially inoculated under controlled conditions. The aim of this work was to determine the optimal inoculum concentration and the best observation period in which the cultivars would show different reactions to bacterial infection as expected on the basis of their field performance.
A suitable statistical analysis of disease indices is proposed to distinguish between resistant and susceptible responses, with a particular care for the applicative needs and a univocal interpretation of the results. In order to evaluate the significance of sources of variation related to the observed mean differences, the analysis of variance and a convenient clustering procedure of disease index means were applied.
The statistical analysis revealed that, under our conditions, an inoculum concentration of 5×106 cfu/plant was suitable for separating resistant from susceptible responses, in accordance with the reactions already observed in field experiments by other authors. Also, differences among the three cultivars were best observed nine to twelve days after inoculation with the pathogen.
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References
Autrique, A. & M.J. Potts, 1987. The influence of mixed cropping on the control of potato bacterial wilt. Ann. Appl. Biol. 111: 125–133.
Calinsky, T. & L.C.A. Corsten, 1984. Clustering means in ANOVA by simultaneous testing. Biometrics 41: 39–48.
Ciampi, L. & L. Sequeira, 1980. Influence of temperature on virulence of race 3 strains of Pseudomonas solanacearum. Am. Pot. J. 57: 307–317.
Ciampi, L., 1984. Bacterial wilt of potato in Chile. Plant Dis. 58: 22–23.
Ciampi-Panno, L., C. Fernandez, P. Bustamante, N. Andrade, S. Ojeda & A. Contreras, 1989. Biological control of bacterial wilt of potatoes caused by Pseudomonas solanacearum. Am. Pot. J. 66: 315–332.
Cole, J.W. & J.E. Grizzle, 1966. Application of multivariate analysis of variance to repeated measures experiments. Biometrics 22: 810–828.
El-Nashaar, H.M., L. De Lindo & U. Nydegger, 1990. A refined mass screening technique for resistance to Pseudomonas solanacearum. Phytopathology 80: 1002 (Abst.).
French, E.R., 1986. Efficacy of five methods of inoculating potato plants with Pseudomonas solanacearum. Phytopathology 76: 1078 (Abstr.).
French, E.R., 1988. Field evaluation of clones bred for resistance to Pseudomonas solanacearum. p. 109–112. In: Bacterial Diseases of the Potato. Report of Planning Conference, CIP, Lima, Peru.
French, E.R. & R. De Lindo, 1982. Resistance to Pseudomonas solanacearum in potato: specificity and temperature sensitivity. Phytopathology 72: 1408–1412.
Gitaitis, R.D., S.R. Ghate, C.A. Jaworsky & S. Phatak, 1983. Evaluation of a mass inoculation method of potatoes with Pseudomonas solanacearum. Am. Pot. J. 60: 625–630.
Gonzalez, L.C., L. Sequeira & P.R. Rowe, 1973. A root inoculation technique to screen potato seedlings for resistance to Pseudomonas solanacearum. Am. Pot. J. 50: 96–104.
Hayward, A.C., 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Ann. Rev. Phytopathol. 29: 65–87.
Hidalgo, O.A. & H. Rincón, 1984. p. 3. In: Marchitez Bacteriana de la Papa (Pseudomonas solanacearum) en America Latina. International Potato Center Press, Lima, Peru.
Jaynes, J.M., K.G. Zanthopoulos, L. Destefano-Beltràn & J.H. Dodds, 1987. Increasing bacterial disease resistance in plants utilising antibacterial genes from insects. Bioassays 6: 263–270.
Kado, E.I. & M.G. Heskett, 1970. Selective media for the isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas and Xanthomonas. Phytopathology 60: 969–976.
Kelman, A., 1954. The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44: 693–695.
Kelman, A., 1981. Brown rot. p. 29 In: W.J. Hooker (Ed). Compendium of Potato Diseases. The American Phytopathological Society.
Kovacs, N., 1956. Identification of Pseudomonas pyocyana by the oxydase reaction. Nature 178: 703.
Mendoza, H.A., 1988. Progress in resistance breeding in potatoes as a function of efficiency of screening procedures. p. 39–64. In: Bacterial Diseases of the Potato. Report of Planning Conference, CIP, Lima, Peru.
Montanelli, C. & G. Nascari, 1991. Introduction of an antibacterial gene in potato (Solanum tuberosum L.) using a binary vector in Agrobacterium rhizogenes. J. Genet. & Breed. 45: 307–316.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473–497.
Scheffé, M., 1959. The analysis of variance. J. Wiley & Sons, New York.
Schmiediche, P., 1988. Breeding for resistance to Pseudomonas solanacearum. p. 19–27. In: Bacterial Diseases of the Potato. Report of Planning Conference, CIP, Lima, Peru.
Tung, P.X., E.T. Rasco Jr., P. Vander Zaag & P. Schmiediche, 1990. Resistance to Pseudomonas solanacearum in the potato: I. Effects of sources of resistance and adaptation. Euphytica 45: 203–210.
Vander Zaag, P., 1986. Potato production under Pseudomonas solanacearum conditions: sources and managment of planting material. p. 84–88. In: G.J. Persley (Ed). Bacterial Wilt Disease in Asia and the South Pacific. Proc. Int. Workshop, PCARRD, Los Banos, Philippines, 8–10 Oct., 1985. ACIAR Proc. 13.
Winstead, N.N. & A. Kelman, 1952. Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology 42: 628–634.
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Montanelli, C., Chiari, A., Chiari, T. et al. Evaluation of resistance to Pseudomonas solanacearum in potato under controlled conditions. Euphytica 81, 35–43 (1995). https://doi.org/10.1007/BF00022457
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DOI: https://doi.org/10.1007/BF00022457