Abstract
Two high (NC106, NC111) and two normal (NC103, NC107) seed protein concentration lines, derived from two different recurrent selection populations of soybean (Glycine max L. Merr.) were subjected to partial defoliation at beginning seed fill (R5) under outdoor pot culture and field conditions. The aim of this study was to test the hypothesis that capacity to store N in vegetative organs and/or to mobilize that N to reproductive organs is associated with the high seed protein concentration trait. Symbiotic N2 fixation was the sole source of N in the pot experiment and the major source of N (met > 50% of the N requirement) in the low N soil used in the field experiment. Seed protein concentration and seed yield at maturity in both experiments and N accumulation and mobilization between R5 and maturity in the pot experiment were measured. The four genotypes did not differ significantly with respect to the amount of N accumulated before beginning seed fill (R5). Removal of up to two leaflets per trifoliolate leaf at R5 significantly decreased the seed protein concentration of NC107/111 but had no effect on this trait in NC103/106. Defoliation treatments significantly decreased seed yield, whole plant N accumulation (N2-fixation) during reproductive growth and vegetative N mobilization of all genotypes. Differences in harvest indices between the high and low protein lines accounted for approximately 35% of the differences in protein concentration. The two normal protein lines mobilized more vegetative N to the seed (average. 5.26 g plant−1) than the two high protein lines (average. 4.28 g plant−1). The two high seed protein lines (NC106, NC111) exhibited significantly different relative dependencies of reproductive N accumulation on vegetative N mobilization, 45% vs. 29%, in the control treatment. Whereas, NC103 with normal and NC106 with high seed protein concentration exhibited similar relative dependencies of reproductive N accumulation on vegetative N mobilization, (47% vs. 45%). Collectively, these results indicate that N stored in shoot organs before R5 and greater absolute and relative contribution of vegetative N mobilization to the reproductive N requirement are not responsible for the high seed protein concentration trait.
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Abbreviations
- DAT:
-
days after transplanting
- R5:
-
fifth reproductive stage according to Fehr and Caviness, 1977
References
Brim C A and Burton J W 1979 Recurrent selection in soybeans. II. Selection for increased percent protein in seeds. Crop Sci. 19, 494–498.
Brun W A 1976 The relation of N2 fixation and photosynthesis. In World Soybean Research. Ed. L DHill. pp 135–143. The Interstate Printers and Publishers, Inc., Danville, IL.
Burkey K O and Wells R 1991 Response of soybean photosynthesis and chloroplast membrane function to canopy development and mutual shading. Plant Physiol. 97, 242–252.
Burton J W 1987 Quantitative genetics: results relevant to soybean breeding. In Soybeans: Improvement, Production, and Use. Agronomy 16, 2nd. ed. Ed. J RWilcox. pp 211–247. American Society of Agronomy, Madison, WI, USA.
Burton J W 1989 Breeding soybeans for increased seed protein percentage. In World Soybean Research Conference IV: Proceedings. Vol II. Ed. A JPascal. pp 1079–1085. Orientación Grafica Editora S R L, Buenos Aires, Argentina.
Burton J W, Wilson R F and Brim C A 1979 Dry matter and nitrogen accumulation in male-sterile and male-fertile soybean. Agron J. 71, 548–552.
CarterJr. T E, Burton J W and Brim C A 1982 Recurrent selection for percent protein in soybean seed: indirect effects on plant N accumulation and distribution. Crop Sci. 22, 513–519.
CarterJr. T E, Burton J W and Brim C A 1986 Registration of NC101 to NC112 soybean germplasm lines contrasting in percent seed protein. Crop Sci. 26, 841–842.
Caviness C E and Thomas J D 1980 Yield reduction from defoliation of irrigated and nonirrigated soybeans. Agron. J. 72, 977–980.
Eckel C S, Bradley J R and VanDuyn J W 1992 Reductions in soybean yield and quality from corn earworm flower feeding. Agron. J. 68, 653–657.
Fehr W R and Caviness C E 1977 Stages of soybean development. Iowa State Univ. Spec. Rep. 80.
Fehr W R, Hicks D R, Hawkins S E, Ford J H and Nelson W W 1983 Soybean recovery from plant cutoff, breakover and defoliation. Agron. J. 75, 512–515.
Glowa W 1974 Zirconium dioxide: A new catalyst in the Kjeldahl method for total N determination. J. Assoc. Off. Anal. Chem. 57, 1228–1230.
Hanson W D, Leffel R C and Howell R W 1961 Genetic analysis of energy production in the soybean. Crop Sci. 1, 121–126.
Hanway J J 1976 Interrelated developmental and biochemical processes in the growth of soybean plants. In World Soybean Research. Ed. L DHill. pp 5–15. The Interstate Printers and Publishers, Inc., Danville, IL.
Henson R A and Heichel G H 1986 Nitrogen accumulation and partitioning in hail-damaged soybeans. J. Plant Nutr. 9, 1453–1468.
Israel D W 1981 Cultivar and Rhizobium strain effects on nitrogen fixation and remobilization by soybean. Agron. J. 73, 509–516.
Israel D W 1991 Biochemical and physiological regulation of nitrogen metabolism in soybean. In Designing Value-Added Soybeans for Markets of the Future. Ed. R FWilson. pp 69–79. American Oil Chemists Society Champaign, Illinois.
Israel D W and Jackson W A 1982 Ion balance, uptake and transport processes in N2-fixing and nitrate- and urea dependent soybean plants. Plant Physiol. 69, 171–179.
Lawn R J and Brun W A 1974 Symbiotic nitrogen fixation in soybeans 1. Effect of photosynthetic source-sink manipulation. Crop Sci. 14, 11–14.
Miller J E and Fehr W R 1979 Direct and indirect recurrent selection for protein in soybeans. Crop. Sci. 19, 101–106.
Mathis J N, Israel D W, Barbour W M, Jarvis B D W and Elkan G H 1986 Analysis of the symbiotic performance of Bradyrhizobium japonicum USDA 110 and its derivative I110 and discovery of a new mannitol-utilizing nitrogen fixing USDA 110 derivative. Appl. Environ. Microbiol. 52, 75–80.
McClure P R and Israel D W 1979 Transport of nitrogen in the xylem of soybean plants. Plant Physiol. 64, 411–416.
Nelson D W and Sommers L E 1973 Determination of total nitrogen in plant material. Agron. J. 65, 109–112.
Salado-Navarro L S, Hinson K and Sinclair T R 1985 Nitrogen partitioning and dry matter allocation in soybeans with different seed protein concentrations. Crop Sci. 25, 451–455.
Sinclair T R and deWit C T 1976 Analysis of the carbon and nitrogen limitations of soybean yield. Agron. J. 68, 319–324.
Singh B B and Hadley H H 1968 Maternal control of oil synthesis in soybeans, Glycine max (L.) Merr. Crop Sci. 8, 622–625.
SAS Institute 1985 Proc. GLM. In SAS Procedures Guide. pp 433–506. Release 5.0 ed. SAS Inst., Cary, NC.
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Burton, J.W., Israel, D.W., Wilson, R.F. et al. Effects of defoliation on seed protein concentration in normal and high protein lines of soybean. Plant Soil 172, 131–139 (1995). https://doi.org/10.1007/BF00020867
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DOI: https://doi.org/10.1007/BF00020867