Abstract
Three water bloom samples were collected in August 1986 from the southern Baltic Sea. Acute toxicity of the samples was determined by mouse bioassay and the toxins were further studied by HPLC. The bloom samples contained equal amounts of cyanobacteria Nodularia spumigena and Aphanizomenon flos-aquae and were hepatotoxic. Two hepatotoxic Nodularia spumigena strains were isolated from the samples. The isolates produce a toxic peak indistinguishable from the bloom material in the HPLC analysis. The toxicity of the fractions was verified by mouse bioassay. Thus the toxicity of the bloom samples was in all likelihood caused by Nodularia spumigena.
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References
Baltic Marine Environment Protection Commission, 1983. Guidelines for the Baltic monitoring program for the second stage. Baltic Sea Environment Proceedings No. 12. 251 pp.
Carmichael, W. W., J. T. Eschedor, G. M. L. Patterson & R. E. Moore, 1988. Toxicity and partial structure of a hepatotoxic peptide produced by the cyanobacterium Nodularia spumigena Mertens emend. L575 from New Zealand. Appl. Environ. Microbiol. 54: 2257–2263.
Edler, L., S. Fernö, M. G. Lind, R. Lundberg & P. O. Nilsson, 1985. Mortality of dogs associated with bloom of the cyanobacterium Nodularia spumigena in the Baltic Sea. Ophelia 24: 103–109.
Eriksson, J. E., J. A. O. Meriluoto, H. P. Kujari, K. Österlund, K. Fagerlund & L. Hällbom, 1988. Preliminary characterization of a toxin isolated from the cyanobacterium Nodularia spumigena. Toxicon 26: 161–166.
Finney, D. J., 1963. Probit Analysis. Rev. ed., Cambridge University Press, London.
Francis, G., 1878. Poisonous Australian lake. Nature 18: 11.
Gussmann, H., M. J. Molzahn & B. Bicks, 1985. Vergiftungen bei Jungrindern durch die Blaualge Nodularia spumigena. Mh. Vet. Med. 40: 76–79.
Huber, A. L., 1984. Nodularia (Cyanobacteriaceae) akinetes in the sediments of the Peel-Harvey Estuary, Western Australia: potential inoculum source for Nodularia blooms. Appl. Envir. Microbiol. 47: 234–238.
Hughes, E. O., P. R. Gorham & A. Zehnder, 1958. Toxicity of a unialgal culture of Microcystis aeruginosa. Can. J. Microbiol. 4: 225–236.
Kotai, J., 1972. Instructions for preparation of modified nutrient solution Z8 for algae. Norwegian Institute for Water Research. B-11/69. Blindern. Oslo. 5 pp.
Lind, M. G., L. Edler, S. Fern, R. Lundberg & P.-O. Nilsson, 1983; Risken for algförgiftning har ökat. Hundar avled efter bad i södra Östersjön. Läkartidningen 80: 2734–2737.
Lindstrøm, E., 1976. Et utbrud of algeforgiftning blandt hunde. Dansk. Vet. Tidsskr. 59: 637–641.
Lundberg, R., L. Edler, S. Fern, M. G. Lind & P. O. Nilsson, 1983. Algförgiftning hos hund. Svensk Veterinärtidning 35: 509–516.
Persson, P. E., K. Sivonen, J. Keto, K. Kononen, M. Niemi & H. Viljamaa, 1984. Potentially toxic blue-green algae (cyanobacteria) in Finnish natural waters. Aqua Fennica 14: 147–154.
Rinehart, K. L., K. Harada, M. Namikoshi, C. Chen & C. A. Harvis, 1988. Nodularin, microcystin, and the configuration of Adda. J. Am. Chem. Soc. 110: 8557–8558.
Rinne, I. & E. Tarkiainen, 1978. Algal tests used to study the chemical factors regulating the growth of planktonic algae in the Helsinki sea area. Mitt. Int. Ver. Limnol. 21: 527–546.
Runnegar, M. T. C., R. B. Jackson, I. R. Falconer, 1988. Toxicity of the cyanobacterium Nodularia spumigena Mertens. Toxicon 26: 143–151.
Siegelman, H. W., W. H. Adams, R. A. Stoner & D. N. Slatkin, 1984. Toxins of Microcystis aeruginosa and their hematological and histopathological effects. In Seafood Toxins, Ragelis, E. P. (ed.), Americal Chemical Society Symposium Series 262, Washington, D.C. 407–413
Tarkiainen, E. & I. Rinne, 1973. Algal assay Procedure used in Water Conservation Laboratory. Reports of the Water Conservation Laboratory 5: 20–21.
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Sivonen, K., Kononen, K., Esala, A.L. et al. Toxicity and isolation of the cyanobacterium Nodularia spumigena from the southern Baltic Sea in 1986. Hydrobiologia 185, 3–8 (1989). https://doi.org/10.1007/BF00006062
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DOI: https://doi.org/10.1007/BF00006062