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Gene Delivery pp 1–22Cite as

Preparation and Evaluation of Boronate-Linked Nanoassembly for Efficient Gene Delivery

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Part of the book series: Biomaterial Engineering ((BIOENG))

Abstract

Rapid transfection can not only maximize the bioavailability of vector-carried gene prior to exocytosis, but also well match chemotherapy for optimal combinational therapy in view of the rapid chemotherapeutic action. However, little attention has been paid to the “rapid” goal in vector-aided transfection process. This chapter describes a boronate-linked nanoassembly for the rapid transnuclear gene transport and efficient gene transfection in vitro and in vivo. The nanoassembly was constructed on the basis of the pH-reversible covalent boronic acid-diol coupling between 1,3-diol-rich oligoethylenimine (OEI-EHDO) and phenylboronic acid modified cholesterol (Chol-PBA). The obtained results demonstrate that the boronate-linked nanoassembly can lead to rapid and efficient nuclei-tropic delivery and transfection, which may largely rely on the lysosome-acidity induced assembly destruction followed by the easy liberation of gene payloads. Consequently, the nanoassembly-mediated transfection just at 8 h can afford the outcome comparable to that achieved at 48 h by the golden standard of PEI25K, and the transfection efficiency can remain at a high level during 48 h. In addition, the in vitro and in vivo results reveal the strong tolerance to the serum interference and the good biocompatibility of this hydroxyl-rich bio-decomposable nanoassembly.

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Author information

Authors and Affiliations

  1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, P. R. China

    Jing-Yi Zhu

  2. Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, P. R. China

    Jing-Yi Zhu, Jun Feng & Xian-Zheng Zhang

Authors
  1. Jing-Yi Zhu
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  2. Jun Feng
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  3. Xian-Zheng Zhang
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Corresponding authors

Correspondence to Jun Feng or Xian-Zheng Zhang .

Editor information

Editors and Affiliations

  1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China

    Huayu Tian

  2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China

    Xuesi Chen

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Zhu, JY., Feng, J., Zhang, XZ. (2021). Preparation and Evaluation of Boronate-Linked Nanoassembly for Efficient Gene Delivery. In: Tian, H., Chen, X. (eds) Gene Delivery. Biomaterial Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-33-6198-0_23-1

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  • DOI: https://doi.org/10.1007/978-981-33-6198-0_23-1

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-33-6198-0

  • Online ISBN: 978-981-33-6198-0

  • eBook Packages: Springer Reference EngineeringReference Module Computer Science and Engineering

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