Minimal Cells and Genome Minimization: Top-Down and Bottom-Up Approaches to Construct Synthetic Cells

Kumar, Nilesh; Samant, Saloni; Singh, Kamini; Reshamwala, Shamlan M. S.

doi:10.1007/978-981-19-7911-8_2

Minimal Cells and Genome Minimization: Top-Down and Bottom-Up Approaches to Construct Synthetic Cells

Nilesh Kumar^3,4,
Saloni Samant⁴,
Kamini Singh^3,4 &
…
Shamlan M. S. Reshamwala⁴

Chapter
First Online: 27 January 2023

701 Accesses

Abstract

Bioproduction of high-value molecules has traditionally been carried out using engineered host cells. However, toxic molecules and membrane proteins are difficult to produce using this approach. Minimal cells, which are synthetic enclosed compartments that mimic the cell, are being explored as an alternative to live cells. Apart from catalysing enzymatic reactions, minimal cells are being constructed that can maintain homeostasis and are also capable of evolution, though in a limited sense. This bottom-up approach is complemented by research in genome minimization, a top-down approach to converting cells into dedicated biofactories. This chapter summarizes studies pertaining to genome minimization and construction of minimal synthetic cells.

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MTech Programme in Bioprocess Technology, Institute of Chemical Technology, Mumbai, India
Nilesh Kumar & Kamini Singh
DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India
Nilesh Kumar, Saloni Samant, Kamini Singh & Shamlan M. S. Reshamwala

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Nilesh Kumar
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Saloni Samant
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Kamini Singh
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Shamlan M. S. Reshamwala
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Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India
Vijai Singh
Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Selangor, Malaysia
Pau Loke Show

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Kumar, N., Samant, S., Singh, K., Reshamwala, S.M.S. (2023). Minimal Cells and Genome Minimization: Top-Down and Bottom-Up Approaches to Construct Synthetic Cells. In: Singh, V., Show, P.L. (eds) Biomanufacturing for Sustainable Production of Biomolecules. Springer, Singapore. https://doi.org/10.1007/978-981-19-7911-8_2

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Published: 27 January 2023
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