Abstract
The overuse of antibiotics will lead to the emergence of drug resistance so that many common diseases can not be effectively treated. Therefore, based on the special structure of graphene oxide (GO) and DNA triple strand, a programmable DNA nanodevice for quantitative detection of tetracycline (TC) is designed in this paper. The introduction of GO as a quencher can effectively reduce the background fluorescence and whether there is any change in the fluorescence in the auxiliary system; Stabilizing the trigger chain with three chain structure will reduce the error more likely. The feasibility analysis preliminarily confirmed the realizability of the designed model, and the optimal conditions were obtained by condition optimization, which laid the foundation for the subsequent quantitative detection of TC, while the selective experiments in different systems fully showed that the model had excellent specificity.
L. Wang and Y. Wang---These authors contributed equally to this work.
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Wang, L., Wang, Y., Hu, M., Xi, S., Cheng, M., Dong, Y. (2022). Graphene Oxide-triplex Structure Based DNA Nanoswitches as a Programmable Tetracycline-Responsive Fluorescent Biosensor. In: Pan, L., Cui, Z., Cai, J., Li, L. (eds) Bio-Inspired Computing: Theories and Applications. BIC-TA 2021. Communications in Computer and Information Science, vol 1565. Springer, Singapore. https://doi.org/10.1007/978-981-19-1256-6_28
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DOI: https://doi.org/10.1007/978-981-19-1256-6_28
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