Abstract
Owing to astonishing properties such as the large surface area to volume ratio, mechanical stability, antimicrobial property, and collagen crosslinking, graphene family nanomaterials (GFNs) have been widely used in various biomedical applications including tissue regeneration. Many review literatures are available to compile the role of GFNs in cardiac, bone, and neuronal tissue regeneration. However, the contribution of GFNs in skin wound healing and tissue regeneration was not yet discussed. In the present review, we have highlighted the properties of GFNs and their application in skin wound healing. In addition, we have included challenges and future directions of GFNs in skin tissue regeneration in the portion of conclusion and perspectives.
Iruthayapandi Selestin Raja and Hee Jeong Jang equally contributed to this work.
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References
Alavi A, Sibbald RG, Mayer D, Goodman L, Botros M, Armstrong DG, Woo K, Boeni T, Ayello EA, Kirsner RS (2014) Diabetic foot ulcers: part I. pathophysiology and prevention. J Am Acad Dermatol 70(1):1. e1–18; quiz 19–20
Anisha BS, Biswas R, Chennazhi KP, Jayakumar R (2013) Chitosan-hyaluronic acid/nano silver composite sponges for drug resistant bacteria infected diabetic wounds. Int J Biol Macromol 62:310–320
Ansell DM, Campbell L, Thomason HA, Brass A, Hardman MJ (2014) A statistical analysis of murine incisional and excisional acute wound models. Wound Repair Regen 22(2):281–287
Arya AK, Tripathi R, Kumar S, Tripathi K (2014) Recent advances on the association of apoptosis in chronic non healing diabetic wound. World J Diabetes 5(6):756–762
Balañá ME, Charreau HE, Leirós GJ (2015) Epidermal stem cells and skin tissue engineering in hair follicle regeneration. World J Stem Cells 7(4):711–727
Bao R, Tan B, Liang S, Zhang N, Wang W, Liu W (2017) A π-π conjugation-containing soft and conductive injectable polymer hydrogel highly efficiently rebuilds cardiac function after myocardial infarction. Biomaterials 122:63–71
Bramini M, Sacchetti S, Armirotti A, Rocchi A, Vázquez E, León Castellanos V, Bandiera T, Cesca F, Benfenati F (2016) Graphene oxide nanosheets disrupt lipid composition, Ca2+ homeostasis, and synaptic transmission in primary cortical neurons. ACS Nano 10(7):7154–7171
Cha C, Shin SR, Gao X, Annabi N, Dokmeci MR, Tang XS, Khademhosseini A (2014) Controlling mechanical properties of cell-laden hydrogels by covalent incorporation of graphene oxide. Small 10(3):514–523
Costa PZ, Soares R (2013) Neovascularization in diabetes and its complications. Unraveling the angiogenic paradox. Life Sci 92(22):1037–1045
Daisy ERAC, Rajendran NK, Houreld NN, Marraiki N, Elgorban AM, Rajan M (2020) Curcumin and Gymnema sylvestre extract loaded graphene oxide-polyhydroxybutyrate-sodium alginate composite for diabetic wound regeneration. React Funct Polym 154:104671
Deepachitra R, Ramnath V, Sastry TP (2014) Graphene oxide incorporated collagen–fibrin biofilm as a wound dressing material. RSC Adv 4(107):62717–62727
Eda G, Fanchini G, Chhowalla M (2008) Large-area ultrathin films of reduced graphene oxide as a transparent and flexible electronic material. Nat Nanotechnol 3(5):270–274
Fan Z, Liu B, Wang J, Zhang S, Lin Q, Gong P, Ma L, Yang S (2014) A novel wound dressing based on Ag/graphene polymer hydrogel: effectively kill bacteria and accelerate wound healing. Adv Funct Mater 24(25):3933–3943
Frontiñán-Rubio J, Gómez MV, MartÃn C, González-DomÃnguez JM, Durán-Prado M, Vázquez E (2018) Differential effects of graphene materials on the metabolism and function of human skin cells. Nanoscale 10(24):11604–11615
Fu J, Zhang Y, Chu J, Wang X, Yan W, Zhang Q, Liu H (2019) Reduced graphene oxide incorporated acellular dermal composite scaffold enables efficient local delivery of mesenchymal stem cells for accelerating diabetic wound healing. ACS Biomater Sci Eng 5(8):4054–4066
Galkowska H, Wojewodzka U, Olszewski WL (2006) Chemokines, cytokines, and growth factors in keratinocytes and dermal endothelial cells in the margin of chronic diabetic foot ulcers. Wound Repair Regen 14(5):558–565
Geim AK (2009) Graphene: status and prospects. Science 324(5934):1530–1534
Geim AK, Novoselov KS (2007) The rise of graphene. Nat Mater 6(3):183–191
Goenka S, Sant V, Sant S (2014) Graphene-based nanomaterials for drug delivery and tissue engineering. J Control Release 173(1):75–88
Guo F, Kim F, Han TH, Shenoy VB, Huang J, Hurt RH (2011) Hydration-responsive folding and unfolding in graphene oxide liquid crystal phases. ACS Nano 5(10):8019–8025
Hong SW, Lee JH, Kang SH, Hwang EY, Hwang Y-S, Lee MH, Han D-W, Park J-C (2014) Enhanced neural cell adhesion and neurite outgrowth on graphene-based biomimetic substrates. Biomed Res Int 2014(1):212149
Jakus AE, Shah RN (2017) Multi and mixed 3D-printing of graphene-hydroxyapatite hybrid materials for complex tissue engineering. J Biomed Mater Res A 105(1):274–283
Kakran M, Sahoo NG, Bao H, Pan Y, Li L (2011) Functionalized graphene oxide as nanocarrier for loading and delivery of ellagic acid. Curr Med Chem 18(29):4503–4512
Kim F, Cote LJ, Huang J (2010a) Graphene oxide: surface activity and two-dimensional assembly. Adv Mater 22(17):1954–1958
Kim J, Cote LJ, Kim F, Yuan W, Shull KR, Huang J (2010b) Graphene oxide sheets at interfaces. J Am Chem Soc 132(23):8180–8186
Komarcević A (2000) The modern approach to wound treatment. Med Pregl 53(7–8):363–368
Lakshmanan R, Maulik N (2018) Graphene-based drug delivery systems in tissue engineering and nanomedicine. Can J Physiol Pharmacol 96(9):869–878
Lee EJ, Lee JH, Shin YC, Hwang D-G, Kim JS, Jin OS, Jin L, Hong SW, Han D-W (2014) Graphene oxide-decorated PLGA/collagen hybrid fiber sheets for application to tissue engineering scaffolds. Biomater Res 18(1):18–24
Lee JH, Shin YC, Jin OS, Kang SH, Hwang Y-S, Park J-C, Hong SW, Han D-W (2015a) Reduced graphene oxide-coated hydroxyapatite composites stimulate spontaneous osteogenic differentiation of human mesenchymal stem cells. Nanoscale 7(27):11642–11651
Lee JH, Shin YC, Lee S-M, Jin OS, Kang SH, Hong SW, Jeong C-M, Huh JB, Han D-W (2015b) Enhanced osteogenesis by reduced graphene oxide/hydroxyapatite nanocomposites. Sci Rep 5(1):18833
Lee H, Paeng K, Kim IS (2018) A review of doping modulation in graphene. Synth Met 244:36–47
Li Y, Liu Y, Fu Y, Wei T, Le Guyader L, Gao G, Liu RS, Chang YZ, Chen C (2012) The triggering of apoptosis in macrophages by pristine graphene through the MAPK and TGF-beta signaling pathways. Biomaterials 33(2):402–411
Li Z, Wang H, Yang B, Sun Y, Huo R (2015) Three-dimensional graphene foams loaded with bone marrow derived mesenchymal stem cells promote skin wound healing with reduced scarring. Mater Sci Eng C Mater Biol Appl 57:181–188
Linares J, Matesanz MC, Vila M, Feito MJ, Gonçalves G, Vallet-Regà M, Marques PA, Portolés MT (2014) Endocytic mechanisms of graphene oxide nanosheets in osteoblasts, hepatocytes and macrophages. ACS Appl Mater Interfaces 6(16):13697–13706
Liu N, Luo F, Wu H, Liu Y, Zhang C, Chen J (2008) One-step ionic-liquid-assisted electrochemical synthesis of ionic-liquid-functionalized graphene sheets directly from graphite. Adv Funct Mater 18(10):1518–1525
Lu B, Li T, Zhao H, Li X, Gao C, Zhang S, Xie E (2012) Graphene-based composite materials beneficial to wound healing. Nanoscale 4(9):2978–2982
Mahmoudi N, Eslahi N, Mehdipour A, Mohammadi M, Akbari M, Samadikuchaksaraei A, Simchi A (2017) Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models. J Mater Sci Mater Med 28(5):73
Mohandas A, Anisha BS, Chennazhi KP, Jayakumar R (2015) Chitosan-hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds. Colloids Surf B Biointerfaces 127:105–113
Novoselov KS, Geim AK, Morozov SV, Jiang D, Zhang Y, Dubonos SV, Grigorieva IV, Firsov AA (2004) Electric field effect in atomically thin carbon films. Science 306(5696):666–669
Nyambat B, Chen C-H, Wong P-C, Chiang C-W, Satapathy MK, Chuang E-Y (2018) Genipin-crosslinked adipose stem cell derived extracellular matrix-nano graphene oxide composite sponge for skin tissue engineering. J Mater Chem B 6(6):979–990
Park S, An J, Jung I, Piner RD, An SJ, Li X, Velamakanni A, Ruoff RS (2009) Colloidal suspensions of highly reduced graphene oxide in a wide variety of organic solvents. Nano Lett 9(4):1593–1597
Park J, Kim YS, Ryu S, Kang WS, Park S, Han J, Jeong HC, Hong BH, Ahn Y, Kim B-S (2015) Graphene potentiates the myocardial repair efficacy of mesenchymal stem cells by stimulating the expression of angiogenic growth factors and gap junction protein. Adv Funct Mater 25(17):2590–2600
Patel S, Srivastava S, Singh MR, Singh D (2019) Mechanistic insight into diabetic wounds: pathogenesis, molecular targets and treatment strategies to pace wound healing. Biomed Pharmacother 112:108615
Pinto AM, Gonçalves IC, Magalhães FD (2013) Graphene-based materials biocompatibility: a review. Colloids Surf B Biointerfaces 111C:188–202
Pumera M (2011) Graphene-based nanomaterials for energy storage. Energy Environ Sci 4(3):668–674
Qiu Z, Kwon AH, Kamiyama Y (2007) Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats. J Surg Res 138(1):64–70
Qiu Y, Wang Z, Owens ACE, Kulaots I, Chen Y, Kane AB, Hurt RH (2014) Antioxidant chemistry of graphene-based materials and its role in oxidation protection technology. Nanoscale 6(20):11744–11755
Qu M, Li Y, Wu Q, Xia Y, Wang D (2017) Neuronal ERK signaling in response to graphene oxide in nematode Caenorhabditis elegans. Nanotoxicology 11(4):520–533
Raja IS, Kang MS, Kim KS, Jung YJ, Han D-W (2020) Two-dimensional theranostic nanomaterials in cancer treatment: state of the art and perspectives. Cancers 12(6):1657
Saravanan S, Chawla A, Vairamani M, Sastry TP, Subramanian KS, Selvamurugan N (2017) Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo. Int J Biol Macromol 104(Pt B):1975–1985
Sasidharan A, Panchakarla LS, Chandran P, Menon D, Nair S, Rao CNR, Koyakutty M (2011) Differential nano-bio interactions and toxicity effects of pristine versus functionalized graphene. Nanoscale 3(6):2461–2464
Sasidharan A, Panchakarla LS, Sadanandan AR, Ashokan A, Chandran P, Girish CM, Menon D, Nair SV, Rao CN, Koyakutty M (2012) Hemocompatibility and macrophage response of pristine and functionalized graphene. Small 8(8):1251–1263
Shahmoradi S, Golzar H, Hashemi M, Mansouri V, Omidi M, Yazdian F, Yadegari A, Tayebi L (2018) Optimizing the nanostructure of graphene oxide/silver/arginine for effective wound healing. Nanotechnology 29(47):475101
Shahnawaz Khan M, Abdelhamid HN, Wu H-F (2015) Near infrared (NIR) laser mediated surface activation of graphene oxide nanoflakes for efficient antibacterial, antifungal and wound healing treatment. Colloids Surf B Biointerfaces 127:281–291
Shams E, Yeganeh H, Naderi-Manesh H, Gharibi R, Mohammad Hassan Z (2017) Polyurethane/siloxane membranes containing graphene oxide nanoplatelets as antimicrobial wound dressings: in vitro and in vivo evaluations. J Mater Sci Mater Med 28(5):75
Shang NG, Papakonstantinou P, McMullan M, Chu M, Stamboulis A, Potenza A, Dhesi SS, Marchetto H (2008) Catalyst-free efficient growth, orientation and biosensing properties of multilayer graphene nanoflake films with sharp edge planes. Adv Funct Mater 18(21):3506–3514
Shang L, Qi Y, Lu H, Pei H, Li Y, Qu L, Wu Z, Zhang W (2019) 7 – Graphene and graphene oxide for tissue engineering and regeneration. In: Cui W, Zhao X (eds) Theranostic bionanomaterials. Elsevier, Amsterdam, pp 165–185
Shao Y, Wang J, Wu H, Liu J, Aksay IA, Lin Y (2010) Graphene based electrochemical sensors and biosensors: a review. Electroanalysis 22(10):1027–1036
Sharp A, Clark J (2011) Diabetes and its effects on wound healing. Nurs Stand 25(45):41–47
Shin SR, Aghaei-Ghareh-Bolagh B, Dang TT, Topkaya SN, Gao X, Yang SY, Jung SM, Oh JH, Dokmeci MR, Tang X, Khademhosseini A (2013) Cell-laden microengineered and mechanically tunable hybrid hydrogels of gelatin and graphene oxide. Adv Mater 25(44):6385–6391
Shin YC, Lee JH, Jin L, Kim MJ, Kim Y-J, Hyun JK, Jung T-G, Hong SW, Han D-W (2015) Stimulated myoblast differentiation on graphene oxide-impregnated PLGA-collagen hybrid fibre matrices. J Nanobiotechnol 13(1):21
Shin SR, Li Y-C, Jang HL, Khoshakhlagh P, Akbari M, Nasajpour A, Zhang YS, Tamayol A, Khademhosseini A (2016) Graphene-based materials for tissue engineering. Adv Drug Deliv Rev 105(Pt B):255–274
Sreenivasulu B, Ramji BR, Nagaral M (2018) A review on graphene reinforced polymer matrix composites. Mater Today Proc 5(1):2419–2428
Takeo M, Lee W, Ito M (2015) Wound healing and skin regeneration. Cold Spring Harb Perspect Med 5(1):a023267
Tamayol A, Akbari M, Annabi N, Paul A, Khademhosseini A, Juncker D (2013) Fiber-based tissue engineering: Progress, challenges, and opportunities. Biotechnol Adv 31(5):669–687
Tan WS, Arulselvan P, Ng SF, Mat Taib CN, Sarian MN, Fakurazi S (2019) Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats. BMC Complement Altern Med 19(1):20
Thangavel P, Kannan R, Ramachandran B, Moorthy G, Suguna L, Muthuvijayan V (2018) Development of reduced graphene oxide (rGO)-isabgol nanocomposite dressings for enhanced vascularization and accelerated wound healing in normal and diabetic rats. J Colloid Interface Sci 517:251–264
Thu HE, Zulfakar MH, Ng SF (2012) Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressing. Int J Pharm 434(1–2):375–383
Tran TH, Nguyen HT, Pham TT, Choi JY, Choi HG, Yong CS, Kim JO (2015) Development of a graphene oxide nanocarrier for dual-drug chemo-phototherapy to overcome drug resistance in cancer. ACS Appl Mater Interfaces 7(51):28647–28655
Weaver CL, Cui XT (2015) Directed neural stem cell differentiation with a functionalized graphene oxide nanocomposite. Adv Healthc Mater 4(9):1408–1416
Yang X, Qiu L, Cheng C, Wu Y, Ma Z-F, Li D (2011) Ordered gelation of chemically converted graphene for next-generation electroconductive hydrogel films. Angew Chem Int Ed 50(32):7325–7328
Yang C, Yan Z, Lian Y, Wang J, Zhang K (2020) Graphene oxide coated shell-core structured chitosan/PLLA nanofibrous scaffolds for wound dressing. J Biomaert Sci Polym Ed 31(5):622–641
Zhao H, Ding R, Zhao X, Li Y, Qu L, Pei H, Yildirimer L, Wu Z, Zhang W (2017) Graphene-based nanomaterials for drug and/or gene delivery, bioimaging, and tissue engineering. Drug Discov Today 22(9):1302–1317
Acknowledgments
This research was supported by National Research Foundation of Korea (NRF) funded by the Ministry of Science (NRF-2019R1A4A1024116) and by Korea Environment Industry & Technology Institute (KEITI) through project to develop eco-friendly new materials and processing technology derived from wildlife, funded by Korea Ministry of Environment (MOE) (2021003270006).
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Raja, I.S. et al. (2022). Role of Graphene Family Nanomaterials in Skin Wound Healing and Regeneration. In: Han, DW., Hong, S.W. (eds) Multifaceted Biomedical Applications of Graphene. Advances in Experimental Medicine and Biology, vol 1351. Springer, Singapore. https://doi.org/10.1007/978-981-16-4923-3_5
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