Abstract
Extremophiles are microorganisms which require or tolerate extreme environmental conditions like high and low temperature, extreme acidic or basic pH, high exposure to radiations, high salinity, low and high pressure, growth in the presence of toxic wastes, organic solvents, heavy metals, and other habitats for their survival. Among different fungal enzymes produced by these extremophiles, lipases are considered to be more valuable natural resource to replace chemical agents for industrial applications. Recombination and immobilization have greatly improved the biocatalytic properties than the native lipases of extremophilic fungal origin. It is more important to screen the molds or yeasts using appropriate methods to determine their lipase producing capability. The most crucial process is the purification of fungal lipases which aid to characterize and distinguish their potential for utilization in various fields of interest. Evaluation of lipolytic activity using different methods provides information about their hydrolyzing property. Extremophilic fungal lipases play indispensable roles in biotechnological applications such as detergent, food and feed, pharmaceuticals, bioremediation, oleochemistry, fine chemicals, textiles, leather, pulp, and paper industries. In this chapter, diverse methods for screening of lipases from fungi isolated from different extreme regions, purification scheme, different lipase assays, and the industrial applications of extremophilic fungal lipases are discussed in detail.
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Angelin, J., Kavitha, M. (2022). Extremophilic Fungal Lipases: Screening, Purification, Assay, and Applications. In: Sahay, S. (eds) Extremophilic Fungi. Springer, Singapore. https://doi.org/10.1007/978-981-16-4907-3_18
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