Skip to main content
SpringerLink
Log in
Book cover

Natural Bio-active Compounds pp 237–264Cite as

Hairy Root Cultures as an Alternative Source for the Production of High-Value Secondary Metabolites

  • Chapter
  • First Online:

Abstract

Hairy roots are rapidly growing, highly differentiated transformed root cultures induced by Agrobacterium rhizogenes infection usually at the infected site of the representative medicinal plant. Hairy roots have the ability to rapidly multiply in the culture medium devoid of any hormones. Unlike other plant cell cultures, hairy root cultures are genetically and biochemically stable and produce a variety of secondary metabolites. In the past three decades, researchers across the world have successfully initiated and cultured hairy roots in vitro for a large number of medicinal plants. Hairy root technology is becoming a promising source for the production of pharmaceutically and industrially important secondary metabolites. This is due to the characteristics of hairy roots, such as rapid growth, the lack of geotropism, extensive lateral branching, and, more importantly, genetic stability. This chapter explores the applications of secondary metabolites in drug formulation, cosmetic preparation, food processing, and the study of plant metabolic pathways. It also briefs about the recent advancements in the area of hairy root culture involving other biotechnological approaches like metabolic engineering or genetic engineering, elicitation, metabolic trapping, and phytoremediation. This chapter certainly benefits the researchers to further explore on the applications of hairy root culturing technology to produce desired plant secondary metabolites on a large scale.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Agostini E, Coniglio MS, Milrad S, Tigier H, Giulietti A (2003) Phytoremediation of 2,4-dichlorophenol by Brassica napus hairy roots cultures. Biotechnol Appl Biochem 37:139–144

    Article  CAS  PubMed  Google Scholar 

  • Ahmadian Chashmi N, Sharifi M, Behmanesh M (2016) Lignan enhancement in hairy root cultures of Linum album using coniferaldehyde and methylenedioxycinnamic acid. Prep Biochem Biotechnol 46:454–460

    Article  CAS  PubMed  Google Scholar 

  • Alderete LGS, Talano MA, Ibáñez SG, Purro S, Agostini E, Milrad SR, Medina MI (2009) Establishment of transgenic tobacco hairy roots expressing basic peroxidases and its application for phenol removal. J Biotechnol 139:273–279

    Article  CAS  Google Scholar 

  • Asada Y, Saito H, Yoshikawa T, Sakamoto K, Furuya T (1993) Biotransformation of 18β-glycyrrhetinic acid by ginseng hairy root culture. Phytochemistry 34:1049–1052

    Article  CAS  PubMed  Google Scholar 

  • Ayadi R, Tremouillaux-Guiller J (2003) Root formation from transgenic calli of Ginkgo biloba. Tree Physiol 23:713–718

    Article  PubMed  Google Scholar 

  • Bais HP, Vepachedu R, Vivanco JM (2003) Root specific elicitation and exudation of fluorescent β-carbolines in transformed root cultures of Oxalis tuberosa. Plant Physiol Biochem 41:345–353

    Article  CAS  Google Scholar 

  • Banerjee S, Rahman L, Uniyal GC, Ahuja PS (1998) Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC. Plant Sci 131:203–208

    Article  CAS  Google Scholar 

  • Banerjee S, Shang TQ, Wilson AM, Moore AL, Strand SE, Gordon MP, Doty SL (2002) Expression of functional mammalian P450 2E1 in hairy root cultures. Biotechnol Bioeng 77:462–466

    Article  CAS  PubMed  Google Scholar 

  • Banerjee S, Singh S, Rahman LU (2012) Biotransformation studies using hairy root cultures – a review. Biotechnol Adv 30:461–468

    Article  CAS  PubMed  Google Scholar 

  • Bathoju G, Rao K, Giri A (2017) Production of sapogenins (stigmasterol and hecogenin) from genetically transformed hairy root cultures of Chlorophytum borivilianum (Safed musli). Plant Cell Tissue Organ Cult 131:369–376

    Article  CAS  Google Scholar 

  • Bensaddek L, Villarreal ML, Fliniaux MA (2008) Induction and growth of hairy roots for the production of medicinal compounds. Electr J Integr Biosci 3:2–9

    Google Scholar 

  • Benson EE, Hamill JD (1991) Cryopreservation and post freeze molecular and biosynthetic stability in transformed roots of Beta vulgaris and Nicotiana rustica. Plant Cell Tissue Organ Cult 24:163–172

    Article  CAS  Google Scholar 

  • Bergier K, Polaszczyk B, Gajewska E, Wielanek M, Krolicka A, Sklodowska M (2008) Glucosylation of hydroquinone to arbutin by hairy roots of Physalis ixocarpa. Zesz Probl Postępów Nauk Rol 2008:524

    Google Scholar 

  • Bhadra R, Wayment DG, Williams RK, Barman SN, Stone MB, Hughes JB, Shanks JV (2001) Studies on plant-mediated fate of the explosives RDX and HMX. Chemosphere 44:1259–1264

    Article  CAS  PubMed  Google Scholar 

  • Bharati AJ, Bansal YK (2014) In vitro production of flavonoids: a review. World J Pharm Pharm Sci 3:508–533

    Google Scholar 

  • Boominathan R, Doran PM (2002) Ni-induced oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii. New Phytol 156:205–215

    Article  CAS  PubMed  Google Scholar 

  • Boominathan R, Doran PM (2003) Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyperaccumulator, Thlaspi caerulescens. Biotechnol Bioeng 83:158–167

    Article  CAS  PubMed  Google Scholar 

  • Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851

    Article  CAS  Google Scholar 

  • Buitelaar RM, Leenen EJTM, Geurtsen G, Tramper J (1993) Effects of the addition of XAD-7 and of elicitor treatment on growth, thiophene production, and excretion by hairy roots of Tagetes patula. Enzyme Microb Technol 15:670–676

    Article  CAS  Google Scholar 

  • Capell T, Christou P (2004) Progress in plant metabolic engineering. Curr Opin Biotechnol 15:148–154

    Article  CAS  PubMed  Google Scholar 

  • Caron D, Coughlan AP, Simard M, Bernier J, Piché Y, Chênevert R (2005) Stereo selective reduction of ketones by Daucus carota hairy root cultures. Biotechnol Lett 27:713–716

    Article  CAS  PubMed  Google Scholar 

  • Casas DA, Pitta-Alvarez SI, Giulietti AM (1998) Biotransformation of hydroquinone by hairy roots of Brugmansia candida and effect of sugars and free-radical scavengers. Appl Biochem Biotechnol 69:127–136

    Article  CAS  PubMed  Google Scholar 

  • Caspeta L, Nieto I, Zamilpa A, Alvarez L, Quintero R, Villarreal ML (2005) Solanum chrysotrichum hairy root cultures: characterization, scale-up and production of five antifungal saponins for human use. Planta Med 71:1084–1087

    Article  CAS  PubMed  Google Scholar 

  • Chandra S, Chandra R (2011) Engineering secondary metabolite production in hairy roots. Phytochem Rev 10:371–395

    Article  CAS  Google Scholar 

  • Chen X, Zhang J, Liu JH, Yu BY (2008) Biotransformation of p-, m-, and o-hydroxybenzoic acids by Panax ginseng hairy root cultures. J Mol Catal B Enzym 54:72–75

    Article  CAS  Google Scholar 

  • Coniglio MS, Busto VD, González PS, Medina MI, Milrad S, Agostini E (2008) Application of Brassica napus hairy root cultures for phenol removal from aqueous solutions. Chemosphere 72:1035–1042

    Article  CAS  PubMed  Google Scholar 

  • Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101:4708–4716

    Article  CAS  PubMed  Google Scholar 

  • De Araujo BS, Charlwood VB, Pletsch M (2002) Tolerance and metabolism of phenol and chloroderivatives by hairy root cultures of Daucus carota L. Environ Pollut 117:329–335

    Article  PubMed  Google Scholar 

  • De Araujo BS, de Oliveira JO, Machado SS, Pletsch M (2004) Comparative studies of peroxidases from hairy roots of Daucus carota, Ipomea batatas and Solanum aviculare. Plant Sci 167:1151–1157

    Article  CAS  Google Scholar 

  • De Araujo BS, Dec J, Bollag JM, Pletsch M (2006) Uptake and transformation of phenol and chlorophenols by hairy root cultures of Daucus carota, Ipomoea batatas and Solanum aviculare. Chemosphere 63:642–651

    Article  PubMed  CAS  Google Scholar 

  • Dechaux C, Boitel-Conti M (2005) A strategy for over accumulation of scopolamine in Datura innoxia hairy root culture. Acta Biol Cracov Ser Bot 47:101–107

    Google Scholar 

  • Donenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzym Microb Technol 17:674–684

    Article  Google Scholar 

  • Dong QF, Jia JZ, Zhu JH, Yu RM (2009) Biotransformation of thymol by hairy roots of transgenic Polygonum multiflorum. J Chin Med Mat 32:1495–1499

    CAS  Google Scholar 

  • Doty SL (2008) Enhancing phytoremediation through the use of transgenics and endophytes. New Phytol 179:318–333

    Article  CAS  PubMed  Google Scholar 

  • Du M, Wu XJ, Ding J, Hu ZB, White KN, Branford-White CJ (2003) Astragaloside IV and polysaccharide production by hairy roots of Astragalus membranaceus in bioreactors. Biotechnol Lett 25:1853–1856

    Article  CAS  PubMed  Google Scholar 

  • Eapen S, Suseelan KN, Tivarekar S, Kotwal SA, Mitra R (2003) Potential for rhizofiltration of uranium using hairy root cultures of Brassica juncea and Chenopodium amaranticolor. Environ Res 91:127–133

    Article  CAS  PubMed  Google Scholar 

  • Ekor M (2014) The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 4:177

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 47:5–16

    Article  Google Scholar 

  • Faria JM, Nunes IS, Figueiredo AC, Pedro LG, Trindade H, Barroso JG (2009) Biotransformation of menthol and geraniol by hairy root cultures of Anethum graveolens: effect on growth and volatile components. Biotechnol Lett 31:897–903

    Article  CAS  PubMed  Google Scholar 

  • Flores HE, Curtis WR (1992) Approaches to understanding and manipulating the biosynthetic potential of plant roots. Ann N Y Acad Sci 665:188–209

    Article  CAS  PubMed  Google Scholar 

  • Fons F, Tousch D, Rapior S, Gueiffier A, Roussel JL, Gargadennec A, Andary C (1999) Phenolic profiles of untransformed and hairy root cultures of Plantago lanceolata. Plant Physiol Biochem 37:291–296

    Article  CAS  Google Scholar 

  • Gabr AM, Mabrok HB, Ghanem KZ, Blaut M, Smetanska I (2016) Lignan accumulation in callus and Agrobacterium rhizogenes-mediated hairy root cultures of flax (Linum usitatissimum). Plant Cell Tissue Organ Cult 126:255–267

    Article  CAS  Google Scholar 

  • Gai QY, Jiao J, Luo M, Wei ZF, Zu YG, Ma W, Fu YJ (2015) Establishment of hairy root cultures by Agrobacterium Rhizogenes mediated transformation of Isatis Tinctoria L. for the efficient production of flavonoids and evaluation of antioxidant activities. PLoS One 10:e0119022

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gao W, Hillwig ML, Huang L, Cui G, Wang X, Kong J, Yang B, Peters RJ (2009) A functional genomics approach to tanshinone biosynthesis provides stereochemical insights. Org Lett 11:5170–5173

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Giri A, Narasu ML (2000) Transgenic hairy roots: recent trends and applications. Biotechnol Adv 18:1–22

    Article  CAS  PubMed  Google Scholar 

  • Giri A, Dhingra V, Giri CC, Singh A, Ward OP, Narasu ML (2001) Biotransformations using plant cells, organ cultures and enzyme systems: current trends and future prospects. Biotechnol Adv 19:175–199

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez PS, Capozucca CE, Tigierm HA, Milrad SR, Agostini E (2006) Phytoremediation of phenol from wastewater, by peroxidases of tomato hairy root cultures. Enzym Microb Technol 39:647–653

    Article  CAS  Google Scholar 

  • Guillon S, Tremouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006) Hairy root research: recent scenario and exciting prospects. Curr Opin Plant Biol 9:341–346

    Article  CAS  PubMed  Google Scholar 

  • Gujarathi NP, Haney BJ, Park HJ, Wickramasinghe SR, Linden JC (2005) Hairy roots of Helianthus annuus: a model system to study phytoremediation of tetracycline and oxytetracycline. Biotechnol Prog 21:775–780

    Article  CAS  PubMed  Google Scholar 

  • Hashemi SM, Naghavi MR (2016) Production and gene expression of morphinan alkaloids in hairy root culture of Papaver orientale (L). using abiotic elicitors. Plant Cell Tissue Organ Cult 125:31–41

    Article  CAS  Google Scholar 

  • Hidalgo D, Georgiev M, Marchev A, Bru-Martínez R, Cusido RM, Corchete P, Palazon J (2017) Tailoring tobacco hairy root metabolism for the production of stilbenes. Sci Rep 7:17976

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Hilton MG, Rhodes MJC (1990) Growth and hyoscyamine production of ‘hairy root’cultures of Datura stramonium in a modified stirred tank reactor. Appl Microb Biotechnol 33:132–138

    Article  CAS  Google Scholar 

  • Hirata K, Goda S, Phunchindawan M, Du D, Ishio M, Sakai A, Miyamoto K (1998) Cryopreservation of horseradish hairy root cultures by encapsulation-dehydration. J Ferment Bioeng 86:418–420

    Article  CAS  Google Scholar 

  • Ho KC, Teow YH, Ang WL, Mohammad AW (2017) An overview of electrically-enhanced membrane bioreactor (embr) for fouling suppression. Int J Eng Sci Rev 10:128–138

    CAS  Google Scholar 

  • Huang Z, Mu Y, Zhou Y, Chen W, Xu K, Yu Z, Bian Y, Yang Q (1997) Transformation of Taxus brevifolia by Agrobacterium rhizogenes and taxol production in hairy root culture. Acta Bot Yunnan 19:292–296

    CAS  Google Scholar 

  • Hussain S, Fareed S, Ansari S, Rahman A, Ahmad IZ, Saeed M (2012) Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sci 4:10–20

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ishimaru K, Yamanaka M, Terahara N, Shimomura K, Okamoto D, Yoshihara K (1996) Biotransformation of phenolics by hairy root cultures of five herbal plants. Jpn J Food Chem Saf 3:38–42

    CAS  Google Scholar 

  • Jaziri M, Legros M, Homes J, Vanhaelen M (1988) Tropine alkaloids production by hairy root cultures of Datura stramonium and Hyoscyamus niger. Phytochemistry 27:419–420

    Article  CAS  Google Scholar 

  • Jeong GT, Park DH (2006) Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system. Appl Biochem Biotechnol 130:436–446

    Article  Google Scholar 

  • Jiao J, Gai QY, Niu LL, Wang XQ, Guo N, Zang YP, Fu YJ (2017) Enhanced production of two bioactive Isoflavone aglycones in Astragalus membranaceus hairy root cultures by combining deglycosylation and elicitation of immobilized edible Aspergillus niger. J Agric Food Chem 65:9078–9086

    Article  CAS  PubMed  Google Scholar 

  • Jung HY, Kang SM, Kang YM, Kang MJ, Yun DJ, Bahk JD, Yang JK, Choi MS (2003a) Enhanced production of scopolamine by bacterial elicitors in adventitious hairy root cultures of Scopolia parviflora. Enzym Microb Technol 33:987–990

    Article  CAS  Google Scholar 

  • Jung JD, Park HW, Hahn Y, Hur CG, In DS, Chung HJ, Liu JR, Choi DW (2003b) Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags. Plant Cell Rep 22:224–230

    Article  CAS  PubMed  Google Scholar 

  • Kanho H, Yaoya S, Itani T, Nakane T, Kawahara N, Takase Y, Masuda K, Kuroyanagi M (2004) Glucosylation of phenolic compounds by Pharbitis nil hairy roots: I. Glucosylation of coumarin and flavone derivatives. Biosci Biotechnol Biochem 68:2032–2039

    Article  CAS  PubMed  Google Scholar 

  • Kanho H, Yaoya S, Kawahara N, Nakane T, Takase Y, Masuda K, Kuroyanagi M (2005) Biotransformation of benzaldehyde-type and acetophenone-type derivatives by Pharbitis nil hairy roots. Chem Pharm Bull 53:361–365

    Article  CAS  Google Scholar 

  • Kawaguchi K, Hirotani M, Yoshikawa T, Furuya T (1990) Biotransformation of digitoxigenin by ginseng hairy root cultures. Phytochemistry 29:837–843

    Article  CAS  PubMed  Google Scholar 

  • Kim Y, Wyslouzil B, Weathers P (2001) A comparative study of mist and bubble column reactors in the in vitro production of artemisinin. Plant Cell Rep 20:451–455

    Article  CAS  Google Scholar 

  • Kim Y, Wyslouzil BE, Weathers PJ (2002) Secondary metabolism of hairy root cultures in bioreactors. In Vitro Cell Dev Biol Plant 38:1–10

    Article  CAS  Google Scholar 

  • Kim SI, Kim JY, Kim EA, Kwon KH, Kim KW, Cho K, Lee JH, Nam MH, Yang DC, Yoo JS, Park YM (2003) Proteome analysis of hairy root from Panax ginseng CA Meyer using peptide fingerprinting, internal sequencing and expressed sequence tag data. Proteomics 3:2379–2392

    Article  CAS  PubMed  Google Scholar 

  • Kim OT, Bang KH, Shin YS, Lee MJ, Jung SJ, Hyun DY, Kim YC, Seong NS, Cha SW, Hwang B (2007) Enhanced production of asiaticoside from hairy root cultures of Centella asiatica (L.) urban elicited by methyl jasmonate. Plant Cell Rep 26:1941–1949

    Article  CAS  PubMed  Google Scholar 

  • Kim H, Popova E, Yi J, Cho G, Park S, Lee S, Engelmann F (2010) Cryopreservation of hairy roots of Rubia akane (Nakai) using a droplet-vitrification procedure. Cryo Lett 31:473–484

    Google Scholar 

  • Kim H, Popova E, Shin D, Bae C, Baek H, Park S, Engelmann F (2012) Development of a droplet-vitrification protocol for cryopreservation of Rubia akane (nakai) hairy roots using a systematic approach. Cryo Lett 33:506–517

    CAS  Google Scholar 

  • Komaraiah P, Reddy GV, Reddy PS, Raghavendra AS, Ramakrishna SV, Reddanna P (2003) Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosum. Biotechnol Lett 25:593–597

    Article  CAS  PubMed  Google Scholar 

  • Kumari M, Chandra S (2017) Secondary metabolite production in transformed cultures. In: Jha S (ed) Transgenesis and secondary metabolism. Springer International Publishing, Cham, pp 103–121

    Chapter  Google Scholar 

  • Kuroyanagi M, Arakava T, Mikami Y, Yoshida K, Kawahar N, Hayashi T, Ishimaru H (1998) Phytoalexins from hairy root culture of Hyoscyamus albus treated with methyl jasmonate. J Nat Prod 61:1516–1519

    Article  CAS  PubMed  Google Scholar 

  • Kwok KH, Doran PM (1995) Kinetic and stoichiometric analysis of hairy roots in a segmented bubble column reactor. Biotechnol Prog 11:429–435

    Article  CAS  Google Scholar 

  • Lambert E, Goossens A, Panis B, Labeke MCV, Geelen D (2009) Cryopreservation of hairy root cultures of Maesa lanceolata and Medicago truncatula. Plant Cell Tissue Organ Cult 96:289–296

    Article  Google Scholar 

  • Largia MJV, Satish L, Johnsi R, Shilpha J, Ramesh M (2016) Analysis of propagation of Bacopa monnieri (L.) from hairy roots, elicitation and Bacoside A contents of Ri transformed plants. World J Microbiol Biotechnol 32:131

    Article  PubMed  CAS  Google Scholar 

  • Le Flem-Bonhomme V, Laurain-Mattar D, Fliniaux MA (2004) Hairy root induction of Papaver somniferum var. album, a difficult-totransform plant by A. rhizogenes LBA 9402. Planta 218:890–893

    Article  PubMed  CAS  Google Scholar 

  • Lee KT, Yamakawa T, Kodama T, Shimomura K (1998) Effects of chemicals on alkaloid production by transformed roots of Atropa belladona. Phytochemistry 49:2343–2347

    Article  CAS  Google Scholar 

  • Lee KT, Suzuki T, Yamakawa T, Kodama T, Igarashi Y, Shimomura K (1999) Production of tropane alkaloids by transformed root cultures of Atropa belladonna in stirred bioreactors with a stainless steel net. Plant Cell Rep 18:567–571

    Article  CAS  Google Scholar 

  • Lee EJ, Park SY, Paek KY (2015a) Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors. Plant Cell Tissue Organ Cult 120:1–10

    Article  CAS  Google Scholar 

  • Lee KJ, Park YK, Kim JY, Jeong TK, Yun KS, Paek KY, Park SY (2015b) Production of biomass and bioactive compounds from adventitious root cultures of Polygonum multiflorum using air-lift bioreactors. Korean J Plant Biotechnol 42:34–42

    Article  Google Scholar 

  • Li W, Koike K, Asada Y, Yoshikawa T, Nikaido T (2003) Biotransformation of low-molecular-weight alcohols by Coleus forskohlii hairy root cultures. Carbohydr Res 338:729–731

    Article  CAS  PubMed  Google Scholar 

  • Li FX, Jin ZP, Zhao DX, Cheng LQ, Fu CX, Ma F (2006) Overexpression of the Saussurea medusa chalcone isomerase gene in S. involucrata hairy root cultures enhances their biosynthesis of apigenin. Phytochemistry 67:553–560

    Article  CAS  PubMed  Google Scholar 

  • Ligang Z, Dechun R, Zhengdan H, Hongtao Z, Chongren Y, Junjian W (1998) Biotransformation of artemisinin by hairy roots of Cyanotis arachnoidea. Acta Bot Yunnan 20:229–232

    Google Scholar 

  • Lin HW, Kwok KH, Doran PM (2003) Development of Linum flavum hairy root cultures for production of coniferin. Biotechnol Lett 25:521–525

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Cheng KD, Zhu P, Feng WH, Meng C, Zhu HX, He HX, Ma XJ (2004) Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus. Acta Pharm Sin 39:445–448

    CAS  Google Scholar 

  • Luczkiewicz M, Kokotkiewicz A (2005) Co-cultures of shoots and hairy roots of Genista tinctoria L. for synthesis and biotransformation of large amounts of phytoestrogens. Plant Sci 169:862–871

    Article  CAS  Google Scholar 

  • Macková M, Macek T, Kučerová P, Burkhard J, Pazlarová J, Demnerová K (1997a) Degradation of polychlorinated biphenyls by hairy root culture of Solanum nigrum. Biotechnol Lett 19:787–790

    Article  Google Scholar 

  • Macková M, Macek T, Ocenaskova J, Burkhard J, Demnerová K, Pazlarová J (1997b) Biodegradation of polychlorinated biphenyls by plant cells. Int Biodeterior Biodegrad 39:317–325

    Article  Google Scholar 

  • Mathur A, Gangwar A, Mathur AK, Verma P, Uniyal GC, Lal RK (2010) Growth kinetics and ginsenosides production in transformed hairy roots of American ginseng-Panax quinquefolium L. Biotechnol Lett 32:457–461

    Article  CAS  PubMed  Google Scholar 

  • Medina-Bolivar F, Condori J, Rimando AM, Hubstenberger J, Shelton K, O’Keefe SF, Dolan MC (2007) Production and secretion of resveratrol in hairy root cultures of peanut. Phytochemistry 68:1992–2003

    Article  CAS  PubMed  Google Scholar 

  • Mehrotra S, Goel MK, Srivastava V, Rahman LU (2015) Hairy root biotechnology of Rauwolfia serpentina: a potent approach for the production of pharmaceutically important terpenoid indole alkaloids. Biotechnol Lett 37:253–263

    Article  CAS  PubMed  Google Scholar 

  • Menzel G, Harloff HJ, Jung C (2003) Expression of bacterial poly (3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.). Appl Microbiol Biotechnol 60:571–576

    Article  CAS  PubMed  Google Scholar 

  • Motomori Y, Shimomura K, Mori K, Kunitake H, Nakashima T, Tanaka M, Miyazaki S, Ishimaru K (1995) Polyphenol production in hairy root cultures of Fragaria × ananassa. Phytochemistry 40:1425–1428

    Article  CAS  Google Scholar 

  • Mukundan U, Hjortso MA (1990) Effect of fungal elicitor on thiophene production in hairy root cultures of Tagetes patula. Appl Microbiol Biotechnol 33:145–147

    Article  CAS  Google Scholar 

  • Murata J, Bienzle D, Brandle JE, Sensen CW, De Luca V (2006) Expressed sequence tags from Madagascar periwinkle (Catharanthus roseus). FEBS Lett 580:4501–4507

    Article  CAS  PubMed  Google Scholar 

  • Murthy HN, Dijkstra C, Anthony P, White DA, Davey MR, Power JB, Hahn EJ, Paek KY (2008) Establishment of Withania somnifera hairy root cultures for the production of Withanolide A. J Integr Plant Biol 50:975–981

    Article  CAS  PubMed  Google Scholar 

  • Murthy HN, Park SY, Paek KY (2017) Production of ginsenosides by hairy root cultures of Panax ginseng. In: Malik S (ed) Production of plant derived natural compounds through hairy root culture. Springer, Cham, pp 203–216

    Chapter  Google Scholar 

  • Namdeo AG (2007) Plant cell elicitation for production of secondary metabolites: a review. Pharmacogn Rev 1:69–79

    CAS  Google Scholar 

  • Nedelkoska TV, Doran PM (2000) Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens. Biotechnol Bioeng 67:607–615

    Article  CAS  PubMed  Google Scholar 

  • Nedelkoska TV, Doran PM (2001) Hyper accumulation of nickel by hairy roots of Alyssum species: comparison with whole regenerated plants. Biotechnol Prog 17:752–759

    Article  CAS  PubMed  Google Scholar 

  • Nunes IS, Faria JM, Figueiredo AC, Pedro LG, Trindade H, Barroso JG (2009) Menthol and geraniol biotransformation and glycosylation capacity of Levisticum officinale hairy roots. Planta Med 75:387–391

    Article  CAS  PubMed  Google Scholar 

  • Oksman-Caldentey KM, Inze D (2004) Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites. Trends Plant Sci 9:433–440

    Article  CAS  PubMed  Google Scholar 

  • Orden AA, Bisogno FR, Cifuente DA, Giordano OS, Sanz MK (2006) Asymmetric bioreduction of natural xenobiotic diketones by Brassica napus hairy roots. J Mol Catal B Enzym 42:71–77

    Article  CAS  Google Scholar 

  • Palazón J, Mallol A, Eibl R, Lettenbauer C, Cusidó RM, Piñol MT (2003) Growth and ginsenoside production in hairy root cultures of Panax ginseng using a novel bioreactor. Planta Med 69:344–349

    Article  PubMed  Google Scholar 

  • Pang Y, Peel GJ, Sharma SB, Tang Y, Dixon RA (2008) A transcript profiling approach reveals an epicatechin-specific glucosyltransferase expressed in the seed coat of Medicago truncatula. Proc Natl Acad Sci U S A 105:14210–14215

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Patra N, Srivastava AK (2016) Artemisinin production by plant hairy root cultures in gas-and liquid-phase bioreactors. Plant Cell Rep 35:143–153

    Article  CAS  PubMed  Google Scholar 

  • Pavlov A, Bley T (2006) Betalains biosynthesis by Beta vulgaris L. hairy root culture in a temporary immersion cultivation system. Process Biochem 41:848–852

    Article  CAS  Google Scholar 

  • Phunchindawan M, Hirata K, Sakai A, Miyamoto K (1997) Cryopreservation of encapsulated shoot primordial induced in horseradish (Armoracia rusticana) hairy root cultures. Plant Cell Rep 16:469–473

    Article  CAS  PubMed  Google Scholar 

  • Putalun W, Taura F, Qing W, Matsushita H, Tanaka H, Shoyama Y (2003) Anti-solasodine glycoside single-chain Fv antibody stimulates biosynthesis of solasodine glycoside in plants. Plant Cell Rep 22:344–349

    Article  CAS  PubMed  Google Scholar 

  • Putalun W, Luealon W, De-Eknamkul W, Tanaka H, Shoyama Y (2007) Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L. Biotechnol Lett 29:1143–1146

    Article  CAS  PubMed  Google Scholar 

  • Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusidó RM, Palazon J (2016) Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21:182. https://doi.org/10.3390/molecules21020182

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Renouard S, Corbin C, Drouet S, Medvedec B, Doussot J, Colas C, Mesnard F (2018) Investigation of Linum flavum (L.) hairy root cultures for the production of anticancer Aryltetralin Lignans. Int J Mol Sci 19:990

    Article  PubMed Central  CAS  Google Scholar 

  • Rhodes MJC, Robins RJ, Hamill JD, Parr AJ, Walton NJ (1987) Secondary product formation using Agrobacterium rhizogenes transformed hairy root cultures. TCA Newsl 53:2–15

    Google Scholar 

  • Rijhwani SK, Shanks JV (1998) Effect of elicitor dosage and exposure time on biosynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol Prog 14:442–449

    Article  CAS  PubMed  Google Scholar 

  • Robbins MP, Hartnoll J, Morris P (1991) Phenylpropanoid defence responses in transgenic Lotus corniculatus 1. Glutathione elicitation of isoflavan phytoalexins in transformed root cultures. Plant Cell Rep 10:59–62

    Article  CAS  PubMed  Google Scholar 

  • Rodriguez-Mendiola MA, Stafford A, Cresswell R, Aria-Castro C (1991) Bioreactors for growth of plant roots. Enzym Microb Technol 13:697–702

    Article  CAS  Google Scholar 

  • Romero F, Delate K, Kraus G, Solco A, Murphy P, Hannapel D (2009) Alkamide production from hairy root cultures of Echinacea. In Vitro Cell Dev Biol Plant 45:599–609

    Article  CAS  Google Scholar 

  • Roychowdhury D, Majumder A, Jha S (2013) Agrobacterium rhizogenes-mediated transformation in medicinal plants: prospects and challenges. In: Chandra S, Latha H, Varma A (eds) Biotechnology for medicinal plants. Springer-Verlag, Berlin, pp 29–68

    Chapter  Google Scholar 

  • Rudrappa T, Bhagyalakshmi N, Ravishankar GA (2004) In situ and ex situ adsorption and recovery of betalains from hairy root cultures of Beta vulgaris. Biotechnol Prog 20:777–785

    Article  CAS  PubMed  Google Scholar 

  • Rudrappa T, Neelwarne B, Kumar V, Lakshmanan V, Venkataramareddy SR, Aswathanarayana RG (2005) Peroxidase production from hairy root cultures of red beet (Beta vulgaris). Electron J Biotechnol 8:66–78

    Article  Google Scholar 

  • Saito K, Sudo H, Yamazaki M, Koseki-Nakamura M, Kitajima M, Takayama H, Aimi N (2001) Feasible production of camptothecin by hairy root culture of Ophiorrhiza pumila. Plant Cell Rep 20:267–271

    Article  CAS  Google Scholar 

  • Salma M, Engelmann-Sylvestre I, Collin M, Escoute J, Lartaud M, Yi J-Y, Kim H, Verdeil J, Engelmann F (2014) Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots. Protoplasma 251:649–659

    Article  CAS  PubMed  Google Scholar 

  • Satdive RK, Fulzele DP, Eapen S (2007) Enhanced production of azadirachtin by hairy root cultures of Azadirachta indica A. Juss by elicitation and media optimization. J Biotechnol 128:281–289

    Article  CAS  PubMed  Google Scholar 

  • Shakeran Z, Keyhanfar M, Asghari G, Ghanadian M (2015) Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel. Turk J Biol 39:111–118

    Article  CAS  Google Scholar 

  • Shanks JV, Morgan J (1999) Plant ‘hairy root’ culture. Curr Opin Biotechnol 10:151–155

    Article  CAS  PubMed  Google Scholar 

  • Sharma S, Shahzad A (2013) Bioreactors: a rapid approach for secondary metabolite production. In: Shahid M, Shahzad A, Malik A, Sahai A (eds) Recent trends in biotechnology and therapeutic applications of medicinal plants. Springer, Dordrecht, pp 25–49

    Chapter  Google Scholar 

  • Sharma P, Padh H, Shrivastava N (2013) Hairy root cultures: a suitable biological system for studying secondary metabolic pathways in plants. Eng Life Sci 13:62–75

    Article  CAS  Google Scholar 

  • Shi HP, Qi Y, Zhang Y, Liang S (2006) Induction of cucumber hairy roots and effect of cytokinin 6-BA on its growth and morphology. Chin J Biotechnol 22:514–520

    CAS  Google Scholar 

  • Shibli RA, Shatnawi MA, Subaih WS, Ajlouni MM (2006) In vitro conservation and cryopreservation of plant genetic resources: a review. World J Agric Sci 2:372–382

    Google Scholar 

  • Shilpha J, Satish L, Kavikkuil M, Largia MJV, Ramesh M (2016) Methyl jasmonate elicits the solasodine production and anti-oxidant activity in hairy root cultures of Solanum trilobatum L. Ind Crop Prod 71:54–64

    Article  CAS  Google Scholar 

  • Singh G (1995) Fungal elicitation of plant root cultures-application to bioreactor dosage. PhD thesis, Pennsylvania State University, USA

    Google Scholar 

  • Singh S, Melo JS, Eapen S, D’Souza SF (2006) Phenol removal using Brassica juncea hairy roots: role of inherent peroxidase and H2O2. J Biotechnol 123:43–49

    Article  CAS  PubMed  Google Scholar 

  • Soudek P, Petrová S, Benesova D, Vanek T (2011) Uranium uptake and stress responses of in vitro cultivated hairy root culture of Armoracia rusticana. Agrochimica 55:15–28

    CAS  Google Scholar 

  • Souret FF, Kim Y, Wyslouzil BE, Wobbe KK, Weathers PJ (2003) Scaleup of Artemisia annua L. hairy root cultures produces complex patterns of terpenoid gene expression. Biotechnol Bioeng 83:653–667

    Article  CAS  PubMed  Google Scholar 

  • Srivastava S, Srivastava AK (2007) Hairy root culture for mass-production of high-value secondary metabolites. Crit Rev Biotechnol 27:29–43

    Article  CAS  PubMed  Google Scholar 

  • Srivastava V, Negi AS, Ajayakumar PV, Khan SA, Banerjee S (2012) Atropa belladonna hairy roots: orchestration of concurrent oxidation and reduction reactions for biotransformation of carbonyl compounds. Appl Biochem Biotechnol 166:1401–1408

    Article  CAS  PubMed  Google Scholar 

  • Staniszewska I, Królicka A, Maliński E, Łojkowska E, Szafranek J (2003) Elicitation of secondary metabolites in in vitro cultures of Ammi majus L. Enzym Microb Technol 33:565–568

    Article  CAS  Google Scholar 

  • Subroto MA, Kwok KH, Hamill JD, Doran PM (1996) Coculture of genetically transformed roots and shoots for synthesis, translocation, and biotransformation of secondary metabolites. Biotechnol Bioeng 49:481–494

    Article  CAS  PubMed  Google Scholar 

  • Subroto MA, Priambodo S, Indrasti NS (2007) Accumulation of zinc by hairy root cultures of Solanum nigrum. Biotechnology 6:344–348

    Article  CAS  Google Scholar 

  • Sudha CG, Obul Reddy B, Ravishankar GA, Seeni S (2003) Production of ajmalicine and ajmaline in hairy root cultures of Rauvolfia micrantha Hook f., a rare and endemic medicinal plant. Biotechnol Lett 25:631–636

    Article  CAS  PubMed  Google Scholar 

  • Sung LS, Huang SY (2006) Lateral root bridging as a strategy to enhance L-DOPA production in Stizolobium hassjoo hairy root cultures by using a mesh hindrance mist trickling bioreactor. Biotechnol Bioeng 94:441–447

    Article  CAS  PubMed  Google Scholar 

  • Suresh B, Sherkhane PD, Kale S, Eapen S, Ravishankar GA (2005) Uptake and degradation of DDT by hairy root cultures of Cichorium intybus and Brassica juncea. Chemosphere 61:1288–1292

    Article  CAS  PubMed  Google Scholar 

  • Suza W, Harris RS, Lorence A (2008) Hairy roots: from high-value metabolite production to phytoremediation. Electron J Integr Biosci 3:57–65

    Google Scholar 

  • Swain SS, Rout KK, Chand PK (2012) Production of triterpenoid anti-cancer compound Taraxerol in Agrobacterium-transformed root cultures of butterfly pea (Clitoria ternatea L.). Appl Biochem Biotechnol 168:487–503

    Article  CAS  PubMed  Google Scholar 

  • Swamy MK, Akhtar MS, Sinniah UR (2016) Response of PGPR and AM fungi toward growth and secondary metabolite production in medicinal and aromatic plants. In: Hakeem KR, Akhtar MS (eds) Plant, soil and microbes: mechanism and molecular interactions-volume 2. Springer, Cham, pp 145–168

    Chapter  Google Scholar 

  • Talano MA, Frontera S, González P, Medina MI, Agostini E (2010) Removal of 2,4-diclorophenol from aqueous solutions using tobacco hairy root cultures. J Hazard Mater 176:784–791

    Article  CAS  PubMed  Google Scholar 

  • Teoh K, Weathers P, Cheetham R, Walcerz D (1996) Cryopreservation of transformed (hairy) roots of Artemisia annua. Cryobiology 33:106–117

    Article  CAS  PubMed  Google Scholar 

  • Thakore D, Srivastava AK, Sinha AK (2017) Mass production of Ajmalicine by bioreactor cultivation of hairy roots of Catharanthus roseus. Biochem Eng J 119:84–91

    Article  CAS  Google Scholar 

  • Thiruvengadam M, Praveen N, Kim EH, Kim S, Chung IM (2014) Production of anthraquinones, phenolic compounds and biological activities from hairy root cultures of Polygonum multiflorum Thunb. Protoplasma 251:555

    Article  CAS  PubMed  Google Scholar 

  • Tian L (2015) Using hairy roots for production of valuable plant secondary metabolites. In: Krull R, Bley T (eds) Filaments in bioprocesses. Springer, Cham, pp 275–324

    Chapter  Google Scholar 

  • Touno K, Yoshimatsu K, Shimomura K (2006) Characteristics of Atropa belladonna hairy roots cryopreserved by vitrification method. Cryo Lett 27:65–72

    CAS  Google Scholar 

  • Vázquez-Flota F, de Lourdes Miranda-Ham M, Castro-Concha L, Tamayo-Ordoñez Y (2017) Synthesis of benzylisoquinoline alkaloids and other tyrosine-derived metabolites in hairy root cultures. In: Malik S (ed) Production of plant derived natural compounds through hairy root culture. Springer, Cham, pp 165–182

    Chapter  Google Scholar 

  • Veena V, Taylor CG (2007) Agrobacterium rhizogenes: recent developments and promising applications. In Vitro Cell Dev Biol Plant 43:383–403

    Article  CAS  Google Scholar 

  • Veersham C, Srinivasan V, Shuler ML (1995) Elicitation of Taxus sp. cell cultures for production of taxol. Biotechnol Lett 17:1343–1346

    Google Scholar 

  • Verpoorte R, Memelink J (2002) Engineering secondary metabolite production in plants. Curr Opin Biotechnol 13:181–187

    Article  CAS  PubMed  Google Scholar 

  • Verpoorte R, Heijden RVD, Hoopen HJGT, Memelink J (1999) Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals. Biotechnol Lett 21:467–479

    Article  CAS  Google Scholar 

  • Vinterhalter B, Savić J, Platiša J, Raspor M, Ninković S, Mitić N, Vinterhalter D (2008) Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit. Plant Cell Tissue Organ Cult 94:299–303

    Article  CAS  Google Scholar 

  • Wang CT, Liu H, Gao XS, Zhang HX (2010) Overexpression of G10H and ORCA3 in the hairy roots of Catharanthus roseus improves catharanthine production. Plant Cell Rep 29:887–894

    Article  CAS  PubMed  Google Scholar 

  • Washida D, Shimomura K, Nakajima Y, Takido M, Kitanaka S (1998) Ginsenosides in hairy roots of a panax hybrid1. Phytochemistry 49:2331–2335

    Article  CAS  Google Scholar 

  • Weathers P, Bunk G, McCoy MC (2005) The effect of phytohormones on growth and artemisinin production in Artemisia annua hairy roots. In Vitro Cell Dev Biol Plant 41:47–53

    Article  CAS  Google Scholar 

  • Wevar-Oller AL, Agostini E, Talano MA, Capozucca C, Milrad SR, Tigier HA, Medina MI (2005) Overexpression of a basic peroxidase in transgenic tomato (Lycopersicon esculentum Mill. cv. Pera) hairy roots increases phytoremediation of phenol. Plant Sci 169:1102–1111

    Article  CAS  Google Scholar 

  • Wielanek M, Królicka A, Bergier K, Gajewska E, Skłodowska M (2009) Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production. Biotechnol Lett 31:917–921

    Article  CAS  PubMed  Google Scholar 

  • Wu J, Lin L (2002) Elicitor-like effects of low-energy ultrasound on plant (Panax ginseng) cells: induction of plant defence responses and secondary metabolite production. Appl Microbiol Biotechnol 59:51–57

    Article  CAS  PubMed  Google Scholar 

  • Xue SH, Luo XJ, Wu ZH, Zhang HL, Wang XY (2008) Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill, Astragalus membranaceus and Gentiana macrophylla Pall. Plant Cell Tissue Organ Cult 92:251–260

    Article  Google Scholar 

  • Yamanaka M, Shimomura K, Sasaki K, Yoshihira K, Ishimaru K (1995) Glucosylation of phenolics by hairy root cultures of Lobelia sessilifolia. Phytochemistry 40:1149–1150

    Article  CAS  Google Scholar 

  • Yan CY, Yu RM, Zhang Z, Kong LY (2007) Biotransformation of 4 hydroxybenzen derivatives by hairy root cultures of Polygonum multiflorum Thunb. J Integr Plant Biol 49:207–212

    Article  CAS  Google Scholar 

  • Yan CY, Ma WL, Yan WW, Yu RM (2008) Biotransformation of furannoligularenone by hairy root cultures of Polygonum multiflorum. J Chin Med Mat 31:633–635

    CAS  Google Scholar 

  • Yang D, Ma P, Liang X, Liang Z, Zhang M, Shen S, Liu H, Liu Y (2012) Metabolic profiles and cDNA-AFLP analysis of Salvia miltiorrhiza and Salvia castanea Diel f. tomentosa Stib. PLoS One 7:e29678

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yang T, Fang L, Nopo-Olazabal C, Condori J, Nopo-Olazabal L, Balmaceda C, Medina-Bolivar F (2015) Enhanced production of resveratrol, piceatannol, arachidin-1, and arachidin-3 in hairy root cultures of peanut co-treated with methyl jasmonate and cyclodextrin. J Agric Food Chem 63:3942–3950

    Article  CAS  PubMed  Google Scholar 

  • Yaoya S, Kanho H, Mikami Y, Itani T, Umehara K, Kuroyanagi M (2004) Umbelliferone released from hairy root cultures of Pharbitis nil treated with copper sulfate and its subsequent glucosylation. Biosci Biotechnol Biochem 68:1837–1841

    Article  CAS  PubMed  Google Scholar 

  • Yoshikawa T, Furuya T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep 6:449–453

    CAS  PubMed  Google Scholar 

  • Yoshikawa T, Asada Y, Furuya T (1993) Continuous production of glycosides by a bioreactor using ginseng hairy root culture. Appl Microbiol Biotechnol 39:460–464

    Article  CAS  Google Scholar 

  • Yoshimatsu K, Yamaguchi H, Shimomura K (1996) Traits of Panax ginseng hairy root after cold storage and cryopreservation. Plant Cell Rep 15:555–560

    Article  CAS  PubMed  Google Scholar 

  • Zhai B, Clark J, Ling T, Connelly M, Medina-Bolivar F, Rivas F (2014) Antimalarial evaluation of the chemical constituents of hairy root culture of Bixa orellana (L). Molecules 19:756–766

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Zhang L, Yang B, Lu B, Kai G, Wang Z, Xia Y, Tang K (2007) Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent. Planta 225:887–896

    Article  CAS  PubMed  Google Scholar 

  • Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  CAS  PubMed  Google Scholar 

  • Zhou LG, Ruan DC, He ZD, Zhu HT, Yang CR, Wang JJ (1998) Biotransformation of artemisinin by hairy roots of Cyanotis arachnoidea. Acta Bot Yunnanica 20:229–232

    CAS  Google Scholar 

  • Zhou ML, Zhu XM, Shao JR, Tang YX, Wu YM (2011) Production and metabolic engineering of bioactive substances in plant hairy root culture. Appl Microbiol Biotechnol 90:1229–1239

    Article  CAS  PubMed  Google Scholar 

  • Zulak KG, Khan MF, Alcantara J, Schriemer DC, Facchini PJ (2009) Plant defense responses in opium poppy cell cultures revealed by liquid chromatography-tandem mass spectrometry proteomics. Mol Cell Proteomics 8:86–98

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The author A. Sagina Rency gratefully acknowledges the Department of Science and Technology for financial support in the form of DST INSPIRE Fellowship (DST/INSPIRE Fellowship/03/2014/004363). Also the authors sincerely thank the Bioinformatics Infrastructure Facility of Department of Biotechnology, Alagappa University (funded by Department of Biotechnology, Government of India: Grant No. BT/BI/25/015/2012), for providing the computational facility.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India

    Arockiam Sagina Rency, Subramani Pandian, Rakkammal Kasinathan, Lakkakula Satish & Manikandan Ramesh

  2. Department of Biotechnology Engineering & The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer Sheva, Israel

    Lakkakula Satish

  3. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia

    Mallappa Kumara Swamy

  4. Department of Biotechnology, East West First Grade College of Science, Bengaluru, Karnataka, India

    Mallappa Kumara Swamy

Authors
  1. Arockiam Sagina Rency
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Subramani Pandian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rakkammal Kasinathan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lakkakula Satish
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mallappa Kumara Swamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Manikandan Ramesh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Manikandan Ramesh .

Editor information

Editors and Affiliations

  1. Department of Botany, Gandhi Faiz-e-Aam College, Shahjahanpur, Uttar Pradesh, India

    Mohd Sayeed Akhtar

  2. Department of Biotechnology, East West First Grade College of Science, Bengaluru, Karnataka, India

    Mallappa Kumara Swamy

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Rency, A.S., Pandian, S., Kasinathan, R., Satish, L., Swamy, M.K., Ramesh, M. (2019). Hairy Root Cultures as an Alternative Source for the Production of High-Value Secondary Metabolites. In: Akhtar, M., Swamy, M. (eds) Natural Bio-active Compounds. Springer, Singapore. https://doi.org/10.1007/978-981-13-7438-8_10

Download citation

Publish with us

Policies and ethics

Search

Navigation