Evaluation of the HBV Genotype, Viral Load and Antivirus Drug Resistance Mutation in Tay Ninh Hospital, Vietnam by Real-Time PCR

Abstract

HBV infection is a leading cause of chronic hepatitis. The new advances in diagnosis and treatment of hepatitis B have significantly contributed in limiting several complications of hepatitis including the usage of molecular biotechnological method such as real-time PCR, typically, which become a helpful method in diagnosis, monitoring, and treatment of disease. The study aimed at developing a real-time PCR assay in order to quantify viral load in blood, genotype and characteristic antiviral drug (Lamivudine—LAM, Adefovir—ADV) resistance mutation in hepatitis B in patients of Tayninh Hospital, Tayninh province, Vietnam. According to results, in Tay Ninh, we certainly recorded several clinical parameters of HBV infections. The patient’s age at HBV infection was 37.5 ± 13.7. Impact of HBV infection in female was counted for 58.5%, the proportion of persons without consideration they are infected with HBV was up to 55.5%, the main of HBV infection through the spread of blood was 94.5%. The ALT value with normal range, HBeAg negative and without any recommendation for hepatitis treatment were counted for 74.5, 71, 68.5% in turn. In HBsAg positive group, 73.5% HBV DNA positive for predominant genotype B was counted for 78.2%. The group with high viral load with ≥20,000 UI/mL was counted for 27.5%. The proportion of antiviral drug resistance mutation in LAM, both LAM and ADV were counted for 67.3, 43.3% in turn. In the group of LAM resistance, double mutations at 204I, 204 V was 52.6%. In cases of ADV resistance, double mutations at 181 V, 181T was reached to the highest proportion with 48.2%. Antiviral drug resistance mutation frequently occurred in the younger group, associated with the high ratio of HBeAg positive, increasing of ALT and high viral load, whereas there is no difference in genotype effected on clinical and subclinical factors.