Abstract
Peroxisomes in fungi are involved in a huge number of different metabolic processes. In addition, non-metabolic functions have also been identified. The proteins that are present in a particular peroxisome determine its metabolic function, whether they are the matrix localized enzymes of the different metabolic pathways or the membrane proteins involved in transport of metabolites across the peroxisomal membrane. Other peroxisomal proteins play a role in organelle biogenesis and dynamics, such as fission, transport and inheritance. Hence, obtaining a complete overview of which proteins are present in peroxisomes at a given time or under a given growth condition provides invaluable insights into peroxisome biology. Bottom up approaches are ideal to follow one or a few proteins at a time but they are not able to give a global view of the content of peroxisomes. To gain such information, top down approaches are required and one that has provided valuable insights into peroxisome function is mass spectrometry based organellar proteomics. Here, we discuss the findings of several such studies in yeast and filamentous fungi and outline new insights into peroxisomal function that were gained from these studies.
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Abbreviations
- APEX:
-
Ascorbate peroxidase
- DDA:
-
Data-dependent Acquisition
- DIA:
-
Data-independent Acquisition
- ESI:
-
Electro-spray Ionization
- GFP:
-
Green fluorescent protein
- GPF:
-
Gas Phase Fractionation
- ICAT:
-
Isotope-coded affinity tags
- MALDI:
-
Matrix Assisted Laser Desorption Ionization
- MS:
-
Mass Spectrometry
- MS/MS:
-
Tandem Mass Spectrometry
- µLC:
-
Micro Liquid chromatography
- nHPLC:
-
High performance liquid chromatography
- nLC:
-
Nano Liquid chromatography
- PMP:
-
Peroxisomal membrane protein
- PNS:
-
Post nuclear supernatant
- PTS:
-
Peroxisomal targeting signal
- ROS:
-
Reactive oxygen species
- SDS-PAGE:
-
Sodium dodecyl sulphate polyacrylamide gel electrophoresis
- SGD:
-
Saccharomyces cerevisiae Genome Database
- SILAC:
-
Stable Isotope Labelling by Amino acids in Cell culture
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Acknowledgements
C.W. is supported by a VIDI Grant (723.013.004) from the Netherlands Organization for Scientific Research (NWO).
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Chen, X., Williams, C. (2018). Fungal Peroxisomes Proteomics. In: del RÃo, L., Schrader, M. (eds) Proteomics of Peroxisomes. Subcellular Biochemistry, vol 89. Springer, Singapore. https://doi.org/10.1007/978-981-13-2233-4_3
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