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Cytomics of Oxidative Stress: Probes and Problems

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Single Cell Analysis

Part of the book series: Series in BioEngineering ((SERBIOENG))

Abstract

Oxidative stress has been implicated in cellular senescence and aging, as well as in the onset and progression of many diverse genetic and acquired diseases and conditions. However, reactive oxygen (ROS) and nitrogen (RNS) species initiating oxidative stress also serve important regulatory roles, mediated by intercellular and intracellular signaling, adaptation to endogenous and exogenous stress, and destruction of invading pathogens. Fluorescence-based analysis of oxidative stress and related processes is an important cytomic application; almost 4000 papers were published between 1989 and 2016. To ascertain the specific role of ROS and RNS in oxidative stress studies by cytomic methodologies, it is essential to detect and characterize these species accurately. Unfortunately, the detection and quantitation of individual intracellular ROS and RNS remains a challenge, but different, complementary cytometric strategies directed toward other endpoints of oxidative stress may also be considered. In this chapter we present and briefly discuss the limitations and perspectives of such approaches.

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Acknowledgements

The authors wish to acknowledge the financial support of the University of Valencia (UVEG) through grants UV-INV-AE15-349700 (Convocatòria Accions Especials 2015) and MOGDETECT (Programa VLC-BIOMED).

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Authors and Affiliations

  1. Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, The University of Valencia and Principe Felipe Research Center, Valencia, Spain

    José-Enrique O’Connor, Beatriz Jávega & Alicia Martínez-Romero

  2. Cytometry Service, Central Research Unit (UCIM), Incliva Foundation, The University of Valencia and University Clinical Hospital, Valencia, Spain

    Guadalupe Herrera

  3. Cytomics Technological Service, Principe Felipe Research Center, Valencia, Spain

    Alicia Martínez-Romero

  4. Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Valencia, Av Blasco Ibáñez, 15, 46010, Valencia, Spain

    José-Enrique O’Connor

  5. Ludwig Institute for Cancer Research, Département D’Oncologie Fondamentale, Faculté de Biologie et Médecine, Université de Lausanne, Épalinges, Switzerland

    Francisco Sala-de-Oyanguren

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  1. José-Enrique O’Connor
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Correspondence to José-Enrique O’Connor .

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  1. Purdue University Cytometry Laboratories, Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA

    J. Paul Robinson

  2. Chair of Pathology and Immunology, Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy

    Andrea Cossarizza

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O’Connor, JE., Herrera, G., Sala-de-Oyanguren, F., Jávega, B., Martínez-Romero, A. (2017). Cytomics of Oxidative Stress: Probes and Problems. In: Robinson, J., Cossarizza, A. (eds) Single Cell Analysis. Series in BioEngineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-4499-1_4

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