Summary
In order to provide future diploidization and production of homozygotic forms for their breeding usage, we studied the opportunity of haploid plant production. Thus, anther culture on nutritional medium was used. The methods developed enabled us to produce heterogenous callus tissues with a frequency up to 60%. Cytological investigations showed that pollen calli had both haploid (up to 30%) and diploid cells, and also tetraploid and aneuploid ones. Techniques and conditions of callus culture have been developed which enable us to obtain plant regenerants for the first time in our experience in Liberty, and Wijcik apple cultivars, in Vladimirskaya and Pamyati Vavilova cherry cvs, in Prunus cerasus × Pr. Maackii and Pr. cerasus × Pr. avium hybrids. We managed to develop special methods for successful adaptation of plant regenerants to natural conditions.
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References
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Zhukov, O.S., O.Ja. Oleynikova & N.I. Savelyev, 1993. Production of plant regenerants by anther cultivation of the most important fruit trees. Second symposium trends in plant biotechnology, Russia, Pushchino, May 18-20, 1993, p. 416.
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© 1994 Springer Science+Business Media Dordrecht
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Zhukov, O.S., Savelyev, N.I., Oleynikova, O.J. (1994). Prospects for application of anther culture in selection of fruit plants. In: Schmidt, H., Kellerhals, M. (eds) Progress in Temperate Fruit Breeding. Developments in Plant Breeding, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0467-8_83
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DOI: https://doi.org/10.1007/978-94-011-0467-8_83
Publisher Name: Springer, Dordrecht
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