Abstract
The rotational diffusion of biological macromolecules is very sensitive to shape, size, and environmental constraints. Large scale and global motions occur in the μ-ms time domain, as in the case of intrinsic membrane proteins. We describe methods for measuring the rotational motion of macromolecules in free, liganded, and cell-surface associated states. They are based on long-lived photophysical processes and the detection of a polarized emission process: phosphorescence, delayed fluorescence, ground state depletion (monitored by fluorescence), and fluorescence from transient intermediates. In every case, the signals derived from different combinations of excitation and emission polarization states are used to construct a time-resolved polarization (anisotropy) function in addition to the primary decay curve. Thus, lifetimes and rotational correlation times can be determined, generally by a multi-component exponential analysis.
Keywords
- Triplet State
- Biological Macromolecule
- Rotational Diffusion
- Rotational Dynamics
- Rotational Correlation Time
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© 1986 D. Reidel Publishing Company
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Blatt, E., Jovin, T.M. (1986). Rotational Dynamics of Biological Macromolecules. In: Winnik, M.A. (eds) Photophysical and Photochemical Tools in Polymer Science. NATO ASI Series, vol 182. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4726-9_15
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DOI: https://doi.org/10.1007/978-94-009-4726-9_15
Publisher Name: Springer, Dordrecht
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