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Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry of Biological Molecules

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Mass Spectrometry in Biomolecular Sciences

Part of the book series: NATO ASI Series ((ASIC,volume 475))

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an analytical technique for fast and precise mass determination of biological molecules. Intact molecular ions are produced by short pulsed laser irradiation of the biomolecules which are enbedded in a matrix consisting of small highly absorbing organic molecules. Mass analysis is carried out in a linear or reflector time-of-flight mass spectrometer. The accessible mass range is 500 000 Da, a mass accuracy of up to 0.01% can be reached, the sample amounts required are 1 pmol or less. Proteins, glycoproteins, oligonucleotides and oligosaccharides can be analyzed.

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Author information

Authors and Affiliations

  1. Institute for Medical Physics and Biophysics, University of Münster, Robert-Koch-Str. 31, 48149, Münster F.R, Germany

    M. Karas & U. Bahr

Authors
  1. M. Karas
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  2. U. Bahr
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Editor information

Editors and Affiliations

  1. Medical School, University of Texas, Houston, Texas, USA

    Richard M. Caprioli

  2. SESMA-C.N.R, Napoli, Italy

    Antonio Malorni

  3. Dipartimento di Chimica, Università della Calabria, Arcavacata di Rende, Italy

    Giovanni Sindona

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© 1996 Kluwer Academic Publishers

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Karas, M., Bahr, U. (1996). Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry of Biological Molecules. In: Caprioli, R.M., Malorni, A., Sindona, G. (eds) Mass Spectrometry in Biomolecular Sciences. NATO ASI Series, vol 475. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0217-6_2

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  • DOI: https://doi.org/10.1007/978-94-009-0217-6_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6581-8

  • Online ISBN: 978-94-009-0217-6

  • eBook Packages: Springer Book Archive

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