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Microbial Metabolic Engineering for L-Threonine Production

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Reprogramming Microbial Metabolic Pathways

Part of the book series: Subcellular Biochemistry ((SCBI,volume 64))

Abstract

L-threonine, one of the three major amino acids produced throughout the world, has a wide application in industry, as an additive or as a precursor for the biosynthesis of other chemicals. It is predominantly produced through microbial fermentation the efficiency of which largely depends on the quality of strains. Metabolic engineering based on a cogent understanding of the metabolic pathways of L-threonine biosynthesis and regulation provides an effective alternative to the traditional breeding for strain development. Continuing efforts have been made in revealing the mechanisms and regulation of L-threonine producing strains, as well as in metabolic engineering of suitable organisms whereby genetically-defined, industrially competitive L-threonine producing strains have been successfully constructed. This review focuses on the global metabolic and regulatory networks responsible for L-threonine biosynthesis, the molecular mechanisms of regulation, and the strategies employed in strain engineering.

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Abbreviations

SD sequence:

Shine-Dalgarno sequence

ACT:

Aspartate kinase, Chorismate mutase and TyrA

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Acknowledgements

This work was supported by the National key Basic Research Program of China (2012CB725202), the Basic Research Programs of Jiangsu Province (BK2009003), and the 111 Project (No. 111-2-06).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, JiangnanUniversity, Wuxi, 214122, China

    Xunyan Dong

  2. Department of Biochemistry, King’s College London, 150 Stamford Street, London, SE1 9NH, United Kingdom

    Peter J. Quinn

  3. State Key Laboratory of Food Science and Technology, School of Biotechnology, JiangnanUniversity, Wuxi, 214122, China

    Xiaoyuan Wang

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  1. Xunyan Dong
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Correspondence to Xiaoyuan Wang .

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  1. , State Key Laboratory, Jiangnan University, Lihu Avenue 1800, Wuxi, 214122, Jiangsu, China, People's Republic

    Xiaoyuan Wang

  2. School of Biotechnology, Jiangnan University, Lihu Avenue 1800, Wuxi, 214122, China, People's Republic

    Jian Chen

  3. , Div. of Life Sciences, King's College London, Stamford Street 150, London, SE1 9NH, United Kingdom

    Peter Quinn

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Dong, X., Quinn, P.J., Wang, X. (2012). Microbial Metabolic Engineering for L-Threonine Production. In: Wang, X., Chen, J., Quinn, P. (eds) Reprogramming Microbial Metabolic Pathways. Subcellular Biochemistry, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5055-5_14

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