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Animal Cell Technology: Basic & Applied Aspects pp 165–170Cite as

Magnetic Cell-Patterning for Tissue Engineering

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Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 16))

Abstract

We report a magnetic cell-patterning technique to control arrangements of target cells for the fabrication of three-dimensional (3D) tissue constructs. The cells labeled with magnetite cationic liposomes (MCLs) could be attracted to the magnetic source. When the magnetic field concentrators were placed underneath the culture substrates, micro-line patterning of human umbilical vein endothelial cells (HUVECs) labeled with MCLs was formed on a monolayer of cells or a magnetic tissue-engineered cell sheet constructed by accumulating MCL-labeled cells uniformly and three-dimensionally to the culture surface using magnetic force. Based on these results, we successfully created multilayered C2C12 cell sheets, where the micro-patterned HUVECs were embedded, by alternating the processes of magnetic accumulation of C2C12 cells for cell layer formation and magnetic patterning of HUVECs onto the cell layers. These results suggested that this cell-patterning technique could be a useful tool for tissue engineering.

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References

  1. Brock, A., Chang, E., Ho, C.C., LeDuc, P., Jiang, X., Whitesides, G.M., and Ingber, D.E. (2003) Geometric determinants of directional cell motility revealed using microcontact printing. Langmuir 19: 1611–1617.

    Article  CAS  PubMed  Google Scholar 

  2. Hui, E.E. and Bhatia, S.N. (2007) Micromechanical control of cell-cell interactions. Proc. Natl. Acad. Sci. U S A. 104: 5722–5726.

    Article  CAS  PubMed  Google Scholar 

  3. Ito, A., Akiyama, H., Kawabe, Y., and Kamihira, M. (2007) Magnetic force-based cell patterning using Arg-Gly-Asp (RGD) peptide-conjugated magnetite cationic liposomes. J. Biosci. Bioeng. 104: 288–293.

    Article  CAS  PubMed  Google Scholar 

  4. Ito, A., Hayashida, M., Honda, H., Hata, K., Kagami, H., Ueda, M., and Kobayashi, T. (2004) Construction and harvest of multilayered keratinocyte sheets using magnetite nanoparticles and magnetic force. Tissue Eng. 10: 873–880.

    Article  CAS  PubMed  Google Scholar 

  5. Ino, K., Ito, A., and Honda, H. (2007) Cell patterning using magnetite nanoparticles and magnetic force. Biotechnol. Bioeng. 97: 1309–1317.

    Article  CAS  PubMed  Google Scholar 

  6. Jiang, X., Xu, Q., Dertinger, S.K., Stroock, A.D., Fu, T.M., and Whitesides, G. M. (2005) A general method for patterning gradients of biomolecules on surfaces using microfluidic networks. Anal. Chem. 15: 2338–2347.

    Article  Google Scholar 

  7. Khademhosseini, A., Langer, R., Borenstein, J., and Vacanti, J.P. (2006) Microscale technologies for tissue engineering and biology. Proc. Natl. Acad. Sci. USA. 103: 2480–2487.

    Article  CAS  PubMed  Google Scholar 

  8. Kobayashi, A., Miyake, H., Hattori, H., Kuwana, R., Hiruma, Y., Nakahama, K., Ichinose, S., Ota, M., Nakamura, M., Takeda, S., and Morita, I. (2007) In vitro formation of capillary networks using optical lithographic techniques. Biochem. Biophys. Res. Commun., 358: 692–697.

    Article  CAS  PubMed  Google Scholar 

  9. Liu Tsang, V., Chen, A.A., Cho, L.M., Jadin, K.D., Sah, R.L., DeLong, S., West, J.L., and Bhatia, S.N. (2007) Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels. FASEB J. 21: 790–801.

    Article  PubMed  Google Scholar 

  10. Nelson, C.M., Jean, R.P., Tan, J.L., Liu, W.F., Sniadecki, N.J., Spector, A.A., and Chen, C.S. (2005) Emergent patterns of growth controlled by multicellular form and mechanics. Proc. Natl. Acad. Sci. U S A. 16: 11571–11572.

    Google Scholar 

  11. Shinkai, M., Yanase, M., Honda, H., Wakabayashi, T., Yoshida, J., and Kobayashi, T. (1996) Intracellular hyperthermia for cancer using magnetite cationic liposomes: in vitro study Jpn. J. Cancer Res. 87: 1179–1183.

    CAS  Google Scholar 

  12. Tsuda, Y., Shimizu, T., Yamato, M., Kikuchi, A., Sasagawa, T., Sekiya, S., Kobayashi, J., Chen, G., and Okano, T. (2007) Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Biomaterials 28: 4939–4946.

    Article  CAS  PubMed  Google Scholar 

  13. Wilson, W.,C., Jr., and Boland, T. (2003) Cell and organ printing 1: protein and cell printers. Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 272: 491–496.

    Article  PubMed  Google Scholar 

  14. Xu, T., Gregory, C. A., Molnar, P., Cui, X., Jalota, S., Bhaduri, S.B., and Boland, T. (2006) Viability and electrophysiology of neural cell structures generated by the inkjet printing method. Biomaterials 27: 3580–3588.

    CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan

    Hirokazu Akiyama, Akira Ito & Yoshinori Kawabe

  2. Graduate School of Systems Life Science, Kyushu University, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

Authors
  1. Hirokazu Akiyama
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  2. Akira Ito
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  3. Yoshinori Kawabe
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  4. Masamichi Kamihira
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Corresponding author

Correspondence to Hirokazu Akiyama .

Editor information

Editors and Affiliations

  1. Fac. Engineering, Dept. Chemical Engineering, Kyushu University, Motooka 744, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

  2. Fac. Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  3. Fac. Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Akira Ito

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Akiyama, H., Ito, A., Kawabe, Y., Kamihira, M. (2010). Magnetic Cell-Patterning for Tissue Engineering. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_27

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