Abstract
Quantitative polymerase chain reaction (QPCR), also known as real-time polymerase chain reaction or kinetic polymerase chain reaction, is a technique used to simultaneously quantify and amplify a specific part of a given DNA molecule. QPCR follows the general procedure of PCR reaction, but the DNA is quantified after each PCR amplification cycle, making the quantification “real-time”. It is used to determine whether or not a specific sequence is present in a specific sample; and if present, the number of copies in the sample. When the QPCR technique is combined with the reverse transcription polymerase chain reaction (RT-PCR), known as quantitative reverse transcription-polymerase chain reaction (QRT-PCR), it enables researchers to quantify low abundance messenger RNA (mRNA) at a particular time, or in a particular cell or tissue type.
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Acknowledgements.
This work was supported in part by Philips Morris USA Inc. and Philips Morris International. We thank Ms. Katherine Long and Ms. Minghua Chai for excellent editorial assistance.
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Zhou, X., Yu, T., Wong, D.T. (2010). Quantitative Reverse Transcription–Polymerase Chain Reaction Based Assessment of the Candidate Biomarkers for Tongue Cancer Metastasis. In: Hayat, M. (eds) Methods of Cancer Diagnosis, Therapy, and Prognosis. Methods of Cancer Diagnosis, Therapy and Prognosis, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3186-0_20
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