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The hepatitis B virus X gene product transactivates the HIV-LTR in vivo

  • Conference paper
Research in Chronic Viral Hepatitis

Part of the book series: Archives of Virology Supplementum ((ARCHIVES SUPPL,volume 8))

Summary

It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demostrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demostrate, for the first time, the in vivo transactivating function of HBx protein.

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Author information

Authors and Affiliations

  1. Laboratoire de Genetique et Patologie Experimentale, INSERM Institut Cochin de Genetique Moleculaire, Paris, France

    Clara Balsano, O. Billet, Myriam Bennoun, Catherine Cavard, A. Zider, Gisele Grimber & P. Briand

  2. Laboratorio di Espressione Genica, Fondazione A. Cesalpino e I Clinica Medica, Policlinico Umberto I, Rome, Italy

    Clara Balsano, G. Natoli & Dr. M. Levrero

Authors
  1. Clara Balsano
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  2. O. Billet
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  3. Myriam Bennoun
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  4. Catherine Cavard
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  5. A. Zider
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  6. Gisele Grimber
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  7. G. Natoli
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  8. P. Briand
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  9. Dr. M. Levrero
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Editor information

Editors and Affiliations

  1. Institut für Medizinische Virologie, Justus-Liebig-Universität Giessen, Giessen, Federal Republic of Germany

    W. H. Gerlich

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© 1993 Springer-Verlag

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Balsano, C. et al. (1993). The hepatitis B virus X gene product transactivates the HIV-LTR in vivo. In: Gerlich, W.H. (eds) Research in Chronic Viral Hepatitis. Archives of Virology Supplementum, vol 8. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9312-9_7

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  • DOI: https://doi.org/10.1007/978-3-7091-9312-9_7

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82497-9

  • Online ISBN: 978-3-7091-9312-9

  • eBook Packages: Springer Book Archive

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