Abstract
Afamin, an 87kDa human plasma glycoprotein with specific binding properties for vitamin E (a-tocopherol) was recently characterized ([Jerkovic, 1997]; [Vögele, 1999]). In the present study the in vitro effects on neuronal cells of native human Afamin, of Afamin pre-loaded with vitamin E (Afamin+), and of vitamin E were investigated. Isolated cortical chicken neurons were maintained either under apoptosis-inducing low serum conditions or exposed to oxidative stress by the addition of H2O2 or beta-amyloid peptide25–35. Afamin and vitamin E synergistically enhance the survival of cortical neurons under apoptotic conditions. Furthermore, Afamin alone protects cortical neurons from cell death in both experimental settings. Therefore, the plasma glycoprotein Afamin apparently displays a neuroprotective activity not only by virtue of binding and transporting vitamin E but also on its own.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aigner A, Wolf S, Gassen HG (1997) Transport und Entgiftung: Ansatze und Perspektiven ftir die Erforschung der Blut-Hirn-Schranke. Angew Chem 109: 25–42
Atabay C, Cagnoli CM, Kharlamov E, Ikonomovic MD, Manev H (1996) Removal of serum from primary cultures of cerebellar granule neurons induces oxidative stress and DNA fragmentation: protection with antioxidants and glutamate receptor antagonists. J Neurosci Res 43: 465–475
Behl C (1999) Vitamin E and other antioxidants in neuroprotection. Int J Vitam Nutr Res 69(3): 213–219
M. Heiser et al.Behl C,Moosmann B,(2000) Estrogen and other antioxidants in neuroprotection: implications for Alzheimer’s disease. In:Poli G,Cadenas E,Packer L (eds) Free radicals in brain pathophysiology,1st edn. Marcel Dekker,New York Basel,p 467
Brera B, Serrano A, Ceballos ML, (2000) 3-amyloid peptides are cytotoxic to astrocytes in culture: a role for oxidative stress. Neurobiol Dis 7: 395–405
Christen Y (2000) Oxidative stress and Alzheimer’s disease. Am J Clin Nutr 71(2): 621–629
Contestabile A (2001) Antioxidant strategies for neurodegenerative diseases. Exp Opin Ther Patents 11(4): 573–585
Dutta-Roy AK (1999) Molecular mechanism of cellular uptake and intracellular translocation of a-tocopherol: role of ocopherol-binding proteins. Food Chem Toxicol 37(9–10): 967–971
Gutmann B, Hutter-Paier B, Skofitsch G, Windisch M, Gmeinbauer R (2002) In vitro models of brain ischemia: the peptidergic drug Cerebrolysin® protects cultured chick cortical neurons from cell death. Neurotox Res 4: 59–65
Herrera E, Barbas C (2001) Vitamin E: action, metabolism and perspectives. J Physiol Biochem 57(2): 43–56
Honig LS, Mayeux R (2001) Natural history of Alzheimer’s disease. Aging Clin Exp Res 13:171–182
Huang HM, Ou HC, Hsieh SJ (2000) Antioxidants prevent amyloid peptide-induced apoptosis and alteration of calcium homeostasis in cultured cortical neurons. Life Sci 66(19): 1879–1892
Hutter-Paier B, Steiner E, Windisch M (1998) Cerebrolysin® protects isolated cortical neurons from neurodegeneration after brief histotoxic hypoxia. J Neural Transm 53: 351–361
Jerkovic L,(1997) Biochemical characterization of a vitamin E-binding protein from human plasma. Thesis, University of Innsbruck
Jimenez-Jimenez FJ, Bustos F, Molina JA, Benito-Leon J, Tallon-Barranco, Gasalla T, Arenas J, Enriquez-de-Salamanca R (1997) Cerebrospinal fluid levels of cc-tocopherol (vitamin E) in Alzheimer’s disease. J Neural Transm 104(6–7): 703–710
Lichenstein HS, Lyons DE, Wurfel MM, Johnson DA, McGinley MD, Leidli JC, Trollinger DB, Mayer JP, Wright SD, ukowski MM (1994) Afamin is a new member of the albumin, a-fetoprotein, and vitamin D-binding protein gene family. J Biol Chem 269(27): 18149–18154
Massoud F, Schittini M, Sano M (2000) Vitamin E and other antioxidant treatments for the neurobehavioral aspects of lzheimer’s disease and other neurodegenerative diseases. In: Poli G, Cadenas E, Packer L (eds) Free radicals in brain pathophysiology, 1st edn. Marcel Dekker, New York Basel, p 501
Mattson MP (2000a) Free radical-mediated disruption of cellular ion homeostasis, mitochondrial dysfunction, and neuronal degeneration in sporadic and inherited Alzheimer’s disease. In: Poli G, Cadenas E, Packer L (eds) Free radicals in brain pathophysiology, 1st edn. Marcel Dekker, New York Basel, p 323
Mattson MP (2000b) Emerging neuroprotective strategies for Alzheimer’s disease: dietary restriction, telomerase activation, and stem cell therapy. Exp Gerontol 35: 489–502
Reinprecht K, Hutter-Paier B, Crailsheim K, Windisch M (1998) Influence of BDNF and FCS on viability and programmed cell death (PCD) of developing cortical chicken neurons in vitro. J Neural Transm 53: 373–384
Ricart KC, Fiszman ML (2001) Hydrogen peroxide-induced neurotoxicity in cultured cortical cells grown in serum-free and serum-containing media. Neurochem Res 26(7): 801–808
Sano M, Ernesto C, Thomas RG, Klauber MR, Schafer K, Grundman M, Woodbury P, Growdon J, Cotman CW, Pfeiffer E, Schneider LS, Thai LJ (1997) A controlled trialNeuroprotective properties of Afamin345of Selegiline, a-tocopherol, or both as treatment for Alzheimer’s disease. N Engl Med 336: 1216–1222
Selkoe DJ (2001) Alzheimer’s disease: genes, proteins and therapy. Physiol Rev 81(2):741–766 Smith MA, Perry G (2000) Molecular and cellular aspects of oxidative damage inAlzheimer’s disease. In: Poli G, Cadenas E, Packer L (eds) Free radicals in brainpathophysiology, 1st edn. Marcel Dekker, New York Basel, p 313
Tabet N, Birks J, Grimley EJ (2000) Vitamin E for Alzheimer’s disease. CochraneDatabase Syst Rev 4: CD002854 Thai LJ (2000) Trials to slow progression and prevent disease onset. J Neural Transm 59:243–249
Traber MG, Sies H, (1996) Vitamin E in humans: demand and delivery. Annu Rev Nutr16: 321–347
Tyas SL,Manfreda J,Strain LA,Montgomery PR,(2001) Risk factors for Alzheimer’s disease: a population-based, longitudinal study in Manitoba,Canada.Int J Epidemiol 30: 590–597
Varadarajan S, Yatin S, Aksenova M, Butterfield DA (2000) Alzheimer’s amyloid (3-peptide-associated free radical oxidative stress and neurotoxicity. J Struct Biol 130:184–208
Vogele k,(1999) Biochemical characterization of the vitamin E-binding properties ofAfamin. Thesis, University of Innsbruck
Wronski R, Kronawetter S, Hutter-Paier B, Crailsheim K, Windisch M, (2000) A brainderived peptide preparation reduces the translation dependent loss of a cytoskeletalprotein in primary cultured chicken neurons. J Neural Transm 59: 263–272
Zimmer S, Stocker A, Sarbolouki MN, Spycher SE, Sassoon J, Azzi A (2000) A novelhuman tocopherol-associated protein: cloning, expression and characterization.J Biol Chem 275(33): 25672–25680
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer-Verlag Wien
About this paper
Cite this paper
Heiser, M. et al. (2002). Vitamin E binding protein A famin Protects Neuronal Cells in vitro . In: Jellinger, K.A., Schmidt, R., Windisch, M. (eds) Ageing and Dementia Current and Future Concepts. Journal of Neural Transmission. Supplementa, vol 62. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6139-5_32
Download citation
DOI: https://doi.org/10.1007/978-3-7091-6139-5_32
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-83796-2
Online ISBN: 978-3-7091-6139-5
eBook Packages: Springer Book Archive