Summary
An in vitro assay system measuring the production of phosphorylated nucleotide products of 5-FU by human tumor cytosols is described. Samples from 11 patients with breast cancer treated with 5-FU-containing chemotherapy regimens were assayed. Significant differences were seen in quantitative and qualitative patterns of phosphorylation between samples from chemotherapy responding and nonresponding patients. The rate of 5-fluorodeoxyuridylate production and the amount of nucleotide triphosphate produced were significantly P < 0.05 increased in chemotherapy-re- sponding patients. This in vitro assay system is a simple, reproducible procedure that requires further evaluation as a potential system for prospectively predicting clinical response to fluorinated pyrimidines.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ardalan B, Macdonald JS, Cooney D, Schein PS (1979) Studies on mechanisms of 5-fluorouracil resistance in murine and human tumors. Bull Cancer 66:55–59
Canellos GP, DeVita VT, Gold GL, Chabner BA, Schein PS, Young RC (1976) Combination chemotherapy for advanced breast cancer: Response and effect on survival. Ann Intern Med 84:389–392
Duschnisky R, Pleven E (1957) The synthesis of 5-fluoro-pyrimidines. J Am Chem Soc 79:4559–4560
Friedman MA, Sadee W (1978) The fluoropyrimidines: Biochemical mechanisms and design of clinical trials. Cancer Chemother Pharmacol 1:77–82
Heidelberger C (1965) Fluorinated pyrimidines. Prog Nucleic Acid Res Mol Biol 4:1–50
Heidelberger C, Chaudhuri NK, Pleven E, Scheiner J (1957) Fluorinated pyrimidines, a new class of tumor-inhibiting compounds. Nature 179:663–666
Heidelberger C, Greisbach L, Montag BJ, Mooren D, Cruz O, Schneizer RJ, Grunberg E (1958) Studies on fluorinated pyrimidines. II. Effects on transplanted tumors. Cancer Res 18:305–317
Livingston RB, Carter SK (1970) Single agents in cancer chemotherapy. Plenum, New York
Roberts D (1966) An isotopic assay for thymidylate synthetase. Biochemistry 5:3546–3548
Sawyer R, Nayak R, Spiegelman S, Martin D (1979) Mechanism of action of 5-fluorouracil (FU) in the chemotherapy of the murine mammary tumor. Proc Am Assoc Cancer Res and Am Soc Clin Oncol 20:263
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Ardalan, B., Macdonald, J., Cooney, D., Lippman, M., Schein, P. (1980). Prediction of Clinical Response to 5-Fluorouracil-Containing Chemotherapy: Preliminary Results of in Vitro Assay in Human Breast Cancer. In: Mathé, G., Muggia, F.M. (eds) Cancer Chemo- and Immunopharmacology. Recent Results in Cancer Research, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81488-4_12
Download citation
DOI: https://doi.org/10.1007/978-3-642-81488-4_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-81490-7
Online ISBN: 978-3-642-81488-4
eBook Packages: Springer Book Archive