Abstract
Methods were developed to monitor graft rejection in a porcine model of unilateral lung transplantation. The ability of peripheral blood mononuclear cells and lavage-derived mononuclear cells to lyse donor pulmonary tissue was determined by standard chromium release assays at various times after transplantation. Effective anti-graft activity was observed in the local environment of a rejecting graft, but not in the periphery. Since transplant rejection is a reversible process, with the administration of suitable immunosuppressive regimes frequently restoring graft function, it was reasoned that immunological assays based on the lysis of individual cells may not be relevant to the in vivo situation. We therefore describe an assay of the lung barrier function; perturbations of the tight intraepithelial junctions which compose the air-blood barrier can be determined in vitro by the measurement of trans-monolayer resistance values.
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References
Higenbottam T, Stewart S, Penketh A, Wallwork J (1988) Transbronchial lung biopsy for the diagnosis of rejection in heart-lung transplant patients. Transplantation 46:532–539
Slieman C, Groussard O, Mai H, Duchatelle J-P, Dubois F, Darne C, Baldeyrou P, Jebrak G (1991) Clinical use of transbronchial biopsy in single lung transplantation. Transplantation 51: 927–928
Kirby J A, Wood A, Reader J A, Isted K, Hynd J, Hawkes D, Hudson L, Pepper JR (1985) Origin and immunological hypo-reactivity of canine alveolar lymphocytes. Immunology 55: 531–537
Kirby JA, Pepper JR, Reader JA, Corbishley CM, Hudson L (1986) Precursor frequency of donor-specific lymphocytes recovered from canine lung transplants. Clin Exp Immunol 63: 334–342
Kirby JA, Parfett GJ, Reader JA, Pepper JR (1988) Lung transplantation in the rat: a model for study of the cellular mechanisms of allograft rejection. Immunology 63:369–372
Rochat T, Casale J, Hunninghake GW, Peterson MW (1988) Neutrophil cathepsin G increases permeability of cultured type II pneumocytes. Am J Physiol 255: C603–C611
Cheek JM, Kim K-J, Crandall ED (1989) Tight monolayers of rat alveolar epithelial cells: bioelectric properties and active sodium transport. Am J Physiol 256: C688–C693
Kirby JA, Morgan JC, Shenton BK, Lennard TWJ, Proud G, Taylor RMR (1991) Renal allograft rejection: functional impairment of kidney epithelial cell monolayers mediated by lympho-kine-activated killer cells and by complement. Transplantation 51:891–895
Boyum A (1968) A one-stage procedure for the isolation of granulocytes and lymphocytes from human blood: general sedimentation properties of white blood cells in a 1 g gravity field. Scand J Clin Lab Invest 21 [Suppl 97]: 77
Brunner KT, Mauel J, Cerrotini J-C, Chapuis B (1968) Quantitative assay on immune lymphoid cells on 51-chromium labelled allogeneic targets in vitro: inhibition by isoantibody and by drugs. Immunology 14:181–187
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© 1992 Springer-Verlag Berlin Heidelberg
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Cunningham, A.C., Kirby, J.A., Colquhoun, I.W., Dark, J.H. (1992). Lung transplantation: pulmonary cell lysis mediated by alveolar mononuclear cells. In: Kootstra, G., Opelz, G., Buurman, W.A., van Hooff, J.P., MacMaster, P., Wallwork, J. (eds) Transplant International Official Journal of the European Society for Organ Transplantation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77423-2_90
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DOI: https://doi.org/10.1007/978-3-642-77423-2_90
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-55342-7
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