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Immediate-Early Transcription Regulation of Human Cytomegalovirus

  • Conference paper
Cytomegaloviruses

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 154))

Abstract

Herpesvirus genomes are expressed in three temporally regulated phases during productive infection. The first period of transcription, commonly termed “immediate early” (IE), follows entry of the virus into the host cell. It is independent of de novo synthesis of viral proteins. In general, a second (“early”) phase follows, when a number of regulatory proteins and viral enzymes are synthesized. During the third phase (“late”), which begins with onset of virion DNA replication, viral structural proteins are synthesized (Honess and Roizman 1974, 1975). Immediate-early proteins are thought to exert important regulatory functions in the switch from the IE to the early phase. If permissive cells are infected in the presence of cycloheximide or anisomycin, both potent inhibitors of protein synthesis, viral IE RNA is accumulated. This approach was applied to investigate IE transcription in various strains of human cytomegalovirus (HCMV) (Wathen and Stinski 1982; McDonough and Spector 1983; Wilkinson et al. 1984; DeMarchi 1981). The IE RNA of HCMV arises from a few distinct regions of the viral genome. The region of highest transcriptional activity is localized between map units 0.66 and 0.77 in the genomes of all HCMV strains that have been investigated (Fig. 1). This region is part of the large unique compartment of the viral genome, for instance corresponding to the HindIII-E fragment of HCMV strain AD169 and the XbaI-E and -N fragments of strain Towne. It comprises approximately 20 kb.

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Authors and Affiliations

  1. Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Loschgestraße 7, D-8520, Erlangen, Germany

    T. Stamminger & B. Fleckenstein

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  1. T. Stamminger
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  2. B. Fleckenstein
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  1. Fred Hutchinson Cancer Research Center, University of Washington, 1124, Columbia Street, Seattle, WA, 98104, USA

    James K. McDougall PhD. (Research Professor of Pathology, Member) (Research Professor of Pathology, Member)

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Stamminger, T., Fleckenstein, B. (1990). Immediate-Early Transcription Regulation of Human Cytomegalovirus. In: McDougall, J.K. (eds) Cytomegaloviruses. Current Topics in Microbiology and Immunology, vol 154. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74980-3_1

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  • DOI: https://doi.org/10.1007/978-3-642-74980-3_1

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