Abstract
Protoplast isolation and regeneration of protoplasts is known from many plants Binding et al. 1981). In spite of great success in producing and handling protoplasts, there are still many plant species which are very resistant against protoplast isolation, or against regeneration. Regengeration of plant protoplasts is more or less a field of testing and trying than understood by known parameters. Therefore great difficulties often accompany the installation of an efficient protoplast regeneration system, even if the isolation provides a high yield of viable protoplasts.
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Hartmann, E., Hock, K. (1985). Fatty Acids in Protoplasts. In: Pilet, PE. (eds) The Physiological Properties of Plant Protoplasts. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70144-3_22
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DOI: https://doi.org/10.1007/978-3-642-70144-3_22
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