Abstract
Glycophorin A (GpA), which is the major sialoglycoprotein on human red cells, is one of the best characterized mammalian integral membrane proteins (Marchesi et al. 1972; Tomita and Marchesi 1975). Its amino acid sequence is known. The protein molecule contains three distinct domains. A large hydrophilic portion, carrying the NH2-terminal, is located on the external surface of the red cell, and the COOH-terminal is located in the cytoplasm (Bretscher 1975) and probably interacts with peripheral proteins on the inner aspect of the membrane. These two hydrophilic sequences are connected by a hydrophobic segment of 23 amino acids which must be embedded within the lipid bilayer.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andersson LC, Gahmberg CG, Teerenhovi L, Vuopio P (1979a) Glycophorin A as a marker of early erythroid differentiation in acute leukemia. Int J Cancer 24:717–720
Andersson LC, Jokinen M, Gahmberg CG (1979b) Induction of erythroid differentiation in the human leukemia cell line K562. Nature 278:364
Andersson LC, Nilsson K, Gahmberg CG (1979c) K562 — a human erythroleukemic cell line. Int J Cancer 23:143–147
Andersson LC, Wegelius R, Borgström GH, Gahmberg CG (1980) Change in cellular phenotype fromlymphoid to erythroid in a case of ALL. Scand J Haematol 24:115–121
Bretscher MS (1975) C-terminal region of the major erythrocyte sialoglycoprotein is on the cytoplasmic side of the membrane. J Mol Biol 98:831–837
Dahr W, Uhlenbruck G, Janssen E, Schmalisch R (1977) Different N-terminal amino acids in MN-glycoprotein from MM and NN erythrocytes. Hum Genet 35:335–340
Gahmberg CG, Hakomori SI (1973) External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem 248:4311–4317
Gahmberg CG, Myllylä G, Leikola A, Pirkola A, Nordling S (1976) Absence of the major sialoglycoprotein in the membrane of human En(a-) erythrocytes and increased glycosylation on band 3. J Biol Chem 251:6108–6116
Gahmberg CG, Jokinen M, Andersson LC (1978) Expression of the major glycoprotein (glycophorin) on erythroid cells in human bone marrow. Blood 52:379–387
Gahmberg CG, Jokinen M, Andersson LC (1979) Expression of the major red cell sialoglycoprotein, glycophorin A, in the human leukemic cell line K562. J Biol Chem 254:7442–7445
Gahmberg CG, Jokinen M, Karhi KK, Andersson LC (1980) Effect of tunicamycin on the biosynthesis of the major human red cell sialoglycoprotein, glycophorin A, in the leukemia cell line K562. J Biol Chem 255:2169–2175
Jokinen M, Gahmberg CG, Andersson LC (1979) Biosynthesis of the major human red cell sialoglycoprotein, glycophorin A, in a continous cell line. Nature 279:604–607
Jokinen M, Ulmanen I, Andersson LC, Kääriäinen L, Gahmberg CG (1981) Cell-free synthesis and glycosylation of the major human red cell sialoglycoprotein, glycophorin A. Eur J Biochem (in press)
Karhi KK, Andersson LC, Vuopio P, Gahmberg CG (1981) Expression of blood group A antigens in human bone marrow cells. Blood 57:147–151
Lisowska E. Duk M (1975) Modification of amino-groups of human erythrocyte glycoproteins and new concept on structure basis of M and N blood-group specificity. Eur J Biochem 54:469–473
Lozzio CB, Lozzio BB (1975) Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45:321–334
Mäkelä O, Cantell K (1958) Destruction of M and N blood group receptors of human red cells by some influenza viruses. Ann Med Exp Fenn 36:366–343
Marchesi VT, Tillack TW, Jackson RL, Segrest JP, Scott RE (1972) Chemical characterization and surface orientation of the major glycoprotein of the human erythrocyte membrane. Proc Natl Acad Sci USA 69:1445–1453
Thomas DB, Winzler RJ (1969) Structural studies on human erythrocyte glycoproteins. J Biol Chem 244:5943–5949
Tomita M, Marchesi VT (1975) Amino acid sequence and Oligosaccharide attachment sites of human erythrocyte glycophorin. Proc Natl Acad Sci USA 73: 2964–2968
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Andersson, L.C., von Willebrand, E., Jokinen, M., Karhi, K.K., Gahmberg, C.G. (1981). Glycophorin A as an Erythroid Marker in Normal and Malignant Hematopoiesis. In: Neth, R., Gallo, R.C., Graf, T., Mannweiler, K., Winkler, K. (eds) Modern Trends in Human Leukemia IV. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67984-1_60
Download citation
DOI: https://doi.org/10.1007/978-3-642-67984-1_60
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-10622-7
Online ISBN: 978-3-642-67984-1
eBook Packages: Springer Book Archive