Abstract:
Microcolony growth procedures have gained recent interest for the following reasons: rapid simultaneous screening for growth of large numbers of bacteria from environmental samples, time-lapse imaging of single-cell growth, cell aging or biofilm studies. Not many of such procedures have been described in sufficient technical detail to be easily reproduced. Here we provide a simple step-by-step procedure, which allows time-lapse imaging of bacterial cell division and cell tracking up to the stage of a monolayered colony of a few 100 cells.
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Reinhard, F., van der Meer, J.R. (2010). Microcolony Growth Procedures. In: Timmis, K.N. (eds) Handbook of Hydrocarbon and Lipid Microbiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77587-4_279
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DOI: https://doi.org/10.1007/978-3-540-77587-4_279
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-77584-3
Online ISBN: 978-3-540-77587-4
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