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Evolving Enzymes for Biocatalysis

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Handbook of Hydrocarbon and Lipid Microbiology

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Abstract:

This article covers concepts developed for the directed evolution of enzymes. The principle strategy is given in comparison to rational protein design followed by a description of the most prominent methods for creation of mutant libraries. Screening and selection strategies to identify the best hits in these libraries are presented followed by several assays developed for a range of enzyme classes. Finally, selected examples for the successful application of evolutionary methods to optimize biocatalysts are given.

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Author information

Authors and Affiliations

  1. Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Greifswald, Germany

    U. T. Bornscheuer

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  1. U. T. Bornscheuer
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Editors and Affiliations

  1. Environmental Microbiology Laboratory, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany

    Kenneth N. Timmis (Professor) (Professor)

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Bornscheuer, U.T. (2010). Evolving Enzymes for Biocatalysis. In: Timmis, K.N. (eds) Handbook of Hydrocarbon and Lipid Microbiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77587-4_217

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