Skip to main content
SpringerLink
Log in
Book cover

Breeding Grasses and Protein Crops in the Era of Genomics pp 249–253Cite as

Use of GBS for Lucerne Variety Distinction

  • Conference paper
  • First Online:

  • 928 Accesses

Abstract

Distinctness among lucerne varieties, evaluated in DUS tests, is a frequent obstacle to variety registration. Promising varieties may fail the distinction test despite an agronomic plus-value so the genetic progress is not delivered to farmers. The Genotyping By Sequencing (GBS) technology that enables to obtain a large number of markers on pools of individuals was used to evaluate the distinction among 20 lucerne varieties of the European Catalogue. A total of 40 000 polymorphic markers was obtained. All the varieties were statistically different from the others, and the structure was consistent with knowledge on the varieties. Perspectives to use GBS in DUS testing are given.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Annicchiarico P, Nazzicari N, Ananta A, Carelli M, Brummer EC (2016) Assessment of cultivar distinctness in alfalfa: a comparison of genotyping-by-sequencing, simple-sequence repeat marker, and morphophysiological observations. Plant Genome 9:1–12

    Article  Google Scholar 

  • Byrne S, Czaban A, Studer B, Panitz F, Bendixen C, Asp T (2013) Genome wide allele frequency fingerprints (GWAFFs) of populations via genotyping by sequencing. PLoS One 8:e57438

    Article  CAS  Google Scholar 

  • Crochemore ML, Huyghe C, Kerlan MC, Durand F, Julier B (1996) Partitioning and distribution of RAPD variation in a set of populations of the Medicago sativa complex. Agronomie 16:421–432

    Article  Google Scholar 

  • Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES, Mitchell SE (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS One 6:e19379

    Article  CAS  Google Scholar 

  • Flajoulot S, Ronfort J, Baudouin P, Barre P, Huguet T, Huyghe C, Julier B (2005) Genetic diversity among alfalfa (Medicago sativa) cultivars coming from a single breeding program, using SSR markers. Theor Appl Genet 111:1420–1429

    Article  CAS  Google Scholar 

  • Raineri E, Ferretti L, Esteve-Codina A, Nevado B, Heath S, Perez-Enciso M (2012) SNP calling by sequencing pooled samples. BMC Bioinform. 13:239

    Article  Google Scholar 

  • UPOV (2013) Guidance of the use of biochemical and molecular markers in the examination of distinctness, uniformity and stability (DUS). http://www.upov.int/edocs/tgpdocs/en/tgp_15.pdf

Download references

Acknowledgments

This study was funded the French Ministry of Agriculture (project CASDAR Amediluze C2014-05). We thank the Genomic Plateform GeT-PlaGe of INRA in Toulouse, France, for sequencing.

Author information

Authors and Affiliations

  1. P3F, INRA, 86600, Lusignan, France

    B. Julier, P. Barre, P. Lambroni & S. Delaunay

  2. GEVES, Domaine de l’Anjouère, 49370, Erdre-en-Anjou, France

    F. Lafaillette

  3. BioGEVES, Domaine du Magneraud, 17700, Surgères, France

    M. Thomasset

  4. GEVES, 711 rue JF Breton, 34090, Montpellier, France

    V. Gensollen

Authors
  1. B. Julier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Barre
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Lambroni
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Delaunay
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. Lafaillette
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Thomasset
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. Gensollen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. Julier .

Editor information

Editors and Affiliations

  1. Lithuanian Research Centre for Agriculture and Forestry (LAMMC), Dotnuva, Lithuania

    Gintaras Brazauskas

  2. Lithuanian Research Centre for Agriculture and Forestry (LAMMC), Dotnuva, Lithuania

    Gražina Statkevičiūtė

  3. Lithuanian Research Centre for Agriculture and Forestry (LAMMC), Dotnuva, Lithuania

    Kristina Jonavičienė

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Julier, B. et al. (2018). Use of GBS for Lucerne Variety Distinction. In: Brazauskas, G., Statkevičiūtė, G., Jonavičienė, K. (eds) Breeding Grasses and Protein Crops in the Era of Genomics. Springer, Cham. https://doi.org/10.1007/978-3-319-89578-9_45

Download citation

Publish with us

Policies and ethics

Search

Navigation