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Cellular and Molecular Toxicology of Nanoparticles pp 163–174Cite as

Nickel Oxide Nanoparticles Induced Transcriptomic Alterations in HEPG2 Cells

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1048))

Abstract

Nickel oxide nanoparticles (NiO-NPs) are increasingly used and concerns have been raised on its toxicity. Although a few studies have reported the toxicity of NiO-NPs, a comprehensive understanding of NiO-NPs toxicity in human cells is still lagging. In this study, we integrated transcriptomic approach and genotoxic evidence to depict the mechanism of NiO-NPs toxicity in human hepatocellular carcinoma (HepG2) cells. DNA damage analysis was done using comet assay, which showed 26-fold greater tail moment in HepG2 cells at the highest concentration of 100 μg/ml. Flow cytometric analysis showed concentration dependent enhancement in intracellular reactive oxygen species (ROS). Real-time PCR analysis of apoptotic (p53, bax, bcl2) and oxidative stress (SOD1) genes showed transcriptional upregulation. Transcriptome analysis using qPCR array showed over expression of mRNA transcripts related to six different cellular pathways. Our data unequivocally suggests that NiO-NPs induces oxidative stress, DNA damage, apoptosis and transcriptome alterations in HepG2 cells.

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Acknowledgements

The authors are grateful to the Deanship of Scientific Research, King Saud University for funding through Vice Deanship of Scientific Research Chairs.

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Authors and Affiliations

  1. Zoology Department, College of Sciences, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Quaiser Saquib & Abdulaziz A. Al-Khedhairy

  2. A.R. Al-Jeraisy Chair for DNA Research, Zoology Department, College of Sciences, King Saud University, P.O. Box 2455, Riyadh, Saudi Arabia

    Quaiser Saquib, Maqsood A. Siddiqui, Javed Ahmad & Rizwan Wahab

  3. Department of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 2455, Riyadh, Saudi Arabia

    Sabiha M. Ansari, Mohammad Faisal & Abdulrahman A. Alatar

  4. Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Javed Musarrat

  5. Department of Biosciences and Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, Jammu and Kashmir, India

    Javed Musarrat

Authors
  1. Quaiser Saquib
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  2. Maqsood A. Siddiqui
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  4. Sabiha M. Ansari
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  7. Abdulrahman A. Alatar
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  8. Abdulaziz A. Al-Khedhairy
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  9. Javed Musarrat
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Corresponding author

Correspondence to Quaiser Saquib .

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Editors and Affiliations

  1. Zoology Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Quaiser Saquib

  2. Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

  3. Zoology Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Abdulaziz A. Al-Khedhairy

  4. Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Abdulrahman A. Alatar

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Saquib, Q. et al. (2018). Nickel Oxide Nanoparticles Induced Transcriptomic Alterations in HEPG2 Cells. In: Saquib, Q., Faisal, M., Al-Khedhairy, A., Alatar, A. (eds) Cellular and Molecular Toxicology of Nanoparticles. Advances in Experimental Medicine and Biology, vol 1048. Springer, Cham. https://doi.org/10.1007/978-3-319-72041-8_10

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