Abstract
Plastic debris represents a significant problem among the various problems facing the marine environment. In this work, we aim to explore the ability of two marine indigenous communities to degrade secondary microplastics. Polyethylene (low-density as well as high-density polyethylene) films were exposed to UV radiation until they were fragmented to microplastics under mild mechanical stress. Next, 50mg of sterile microplastics with size 2 mm–250 μm was added into sterile flasks and was incubated separately with these two pelagic microbiomes. A significant decrease in the weight of microplastics was determined along the experimental period, implying the potential ability of indigenous communities to in situ degrade secondary microplastics. Moreover, the protein content marginally decreased while carbohydrate content of both treatments increased at this time interval. Accordingly, the populations increased along experimental period.
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References
Avio, C.G., Gorbi, S., Regoli, F.: Plastics and microplastics in the oceans: from emerging pollutants to emerged threat. Mar. Environ. Res. (2016) Available from: http://dx.doi.org/10.1016/j.marenvres.2016.05.012
Ryan, P.G., Moore, C.J., van Franeker, J., Moloney, C.L.: Monitoring the abundance of plastic debris in the marine environment. Philos. Trans. R. Soc. Lond. B Biol. Sci. 364, 1999–2012 (2009)
Barboza, L.G.A., Gimenez, B.C.G.: Microplastics in the marine environment: current trends and future perspectives. Mar. Pollut. Bull. 97(1–2), 5–12 (2015)
Wright, S.L., Thompson, R.C., Galloway, T.S.: The physical impacts of microplastics on marine organisms: a review. Environ. Pollut. 178, 483–492 (2013)
Wang, J., Tan, Z., Peng, J., Qiu, Q., Li, M.: The behaviors of microplastics in the marine environment. Mar. Environ. Res. 113, 7–17 (2016)
Eriksen, M., Lebreton, L.C.M., Carson, H.S., Thiel, M., Moore, C.J., Borerro, J.C., et al.: Plastic pollution in the world’s oceans: more than 5 trillion plastic pieces weighing over 250,000 tons afloat at sea. PLoS ONE 9(12), 1–15 (2014)
da Costa, J.P., Santos, P.S.M., Duarte, A.C., Rocha-Santos, T.: (Nano)plastics in the environment—Sources, fates and effects. Sci. Total Environ. 566–567, 15–26 (2016)
Arthur, C., Baker, J., Bamford, H.: Proceedings of the International Research Workshop on the Occurrence, Effects, and Fate of Microplastic Marine Debris. Group. (2009) (January):530
Cole, M., Lindeque, P., Halsband, C., Galloway, T.S.: Microplastics as contaminants in the marine environment: a review. Mar. Pollut. Bull. 62(12), 2588–2597 (2011)
Andrady, A.L.: Microplastics in the marine environment. Mar. Pollut. Bull. 62(8), 1596–1605 (2011)
Andrady, A.L.: Environmental degradation of plastics under land and marine exposure conditions. In: Second International Conference Marine Debris. 1990, pp. 848–869 (April 1989)
Jabarin, S.A., Lofgren, E.A.: Photooxidative effects on properties and structure of high density polyethylene. J. Appl. Polym. Sci. 53(4), 411–423 (1994)
Tidjani, A.: Comparison of formation of oxidation products during photo-oxidation of linear low density polyethylene under different natural and accelerated weathering conditions. Polym. Degrad. Stab. 68(3), 465–469 (2000)
Carrasco, F., Pagès, P., Pascual, S., Colom, X.: Artificial aging of high-density polyethylene by ultraviolet irradiation. Eur. Polym. J. 37(7), 1457–1464 (2001)
O’Brine, T., Thompson, R. C.: Degradation of plastic carrier bags in the marine environment. Mar. Pollut. Bull., 60(12), 2279–2283 (2010)
Kalogerakis, N., Karkanorachaki, K., Kalogerakis, G.C., Triantafyllidi, E.I., Gotsis, A.D., Partsinevelos, P., et al.: Microplastics generation: onset of fragmentation of polyethylene films in marine environment mesocosms. Front. Mar. Sci. 4, 1–15 (2017)
European Council No Title. p. 371 (2013)
PlasticsEurope. Plastics—the facts 2014/2015: An analysis of European plastics production, demand and waste data. PlasticsEurope, pp. 1–34 (2015)
Corcoran, P.L., Biesinger, M.C., Grifi, M.: Plastics and beaches: A degrading relationship. Mar. Pollut. Bull. 58(1), 80–84 (2009)
Hidalgo-Ruz, V., Thiel, M.: Distribution and abundance of small plastic debris on beaches in the SE Pacific (Chile): a study supported by a citizen science project. Mar. Environ. Res. 87–88, 12–18 (2013)
Restrepo-Flórez, J.M., Bassi, A., Thompson, M.R.: Microbial degradation and deterioration of polyethylene—a review. Int. Biodeterior. Biodegradation 88, 83–90 (2014)
Kumar Sen, S., Raut, S.: Microbial degradation of low density polyethylene (LDPE): a review. J. Environ. Chem. Eng. 3(1), 462–473 (2015)
Perez-de-Mora, A., Engel, M., Schloter, M.: Abundance and diversity of n-alkane-degrading bacteria in a forest soil co-contaminated with hydrocarbons and metals: a molecular study on alkb homologous genes. Microb. Ecol. 62(4), 959–972 (2011)
Jin, C.E., Kim, M.N.: Change of bacterial community in oil-polluted soil after enrichment cultivation with low-molecular-weight polyethylene. Int. Biodeterior. Biodegradation 118, 27–33 (2017)
Cozar, A., Echevarria, F., Gonzalez-Gordillo, J.I., Irigoien, X., Ubeda, B., Hernandez-Leon, S., et al.: Plastic debris in the open ocean. Proc. Natl. Acad. Sci. 111(28), 10239–10244 (2014)
Ter Halle, A., Ladirat, L., Gendre, X., Goudouneche, D., Pusineri, C., Routaboul, C., et al.: Understanding the fragmentation pattern of marine plastic debris. Environ. Sci. Technol. 50(11), 5668–5675 (2016)
Sudhakar, M., Doble, M., Murthy, P.S., Venkatesan, R.: Marine microbe-mediated biodegradation of low- and high-density polyethylenes. Int. Biodeterior. Biodegradation 61(3), 203–213 (2008)
Skariyachan, S., Manjunatha, V., Sultana, S., Jois, C., Bai, V., Vasist, K.S.: Novel bacterial consortia isolated from plastic garbage processing areas demonstrated enhanced degradation for low density polyethylene. Environ. Sci. Pollut. Res. 23(18), 18307–18319 (2016)
Sivan, A.: New perspectives in plastic biodegradation. Curr. Opin. Biotechnol. 22(3), 422–426 (2011)
Sheng, G.P., Yu, H.Q., Li, X.Y.: Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol. Adv. 28(6), 882–894 (2010)
Reisser, J., Shaw, J., Hallegraeff, G., Proietti, M., Barnes, D.K.A., Thums, M., et al.: Millimeter-sized marine plastics: a new pelagic habitat for microorganisms and invertebrates. PLoS ONE 9(6), 1–11 (2014)
Zettler, E.R., Mincer, T.J., Amaral-Zettler, L.: Life in the “Plastisphere”: Microbial communities on plastic marine debris. Environ. Sci. Technol. 47, 7137–7146 (2013)
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Tsiota, P., Karkanorachaki, K., Syranidou, E., Franchini, M., Kalogerakis, N. (2018). Microbial Degradation of HDPE Secondary Microplastics: Preliminary Results. In: Cocca, M., Di Pace, E., Errico, M., Gentile, G., Montarsolo, A., Mossotti, R. (eds) Proceedings of the International Conference on Microplastic Pollution in the Mediterranean Sea. Springer Water. Springer, Cham. https://doi.org/10.1007/978-3-319-71279-6_24
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DOI: https://doi.org/10.1007/978-3-319-71279-6_24
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