Abstract
Rapid detection technologies with high sensitivity and selectivity for plant pathogens are essential to prevent disease spread with minimal loss to crop production and food quality assurance. Traditional laboratory techniques such as microscopic and cultural techniques are time-consuming and require complex sample handling. Immunological and molecular techniques are advanced but have some issues related to rapidity and signal strength. In this context, integration of immunological and molecular diagnostics with nanotechnology systems offers an alternative where all detection steps are done by a portable miniaturized device for rapid and accurate identification of plant pathogens. Further, nanomaterial synthesis by utilizing functionalized metal nanoparticles as a sensing component offer several desirable features required for pathogen detection. The sensitive nature of functionalized nanoparticles can be utilized to design phytopathogen detection devices with smart sensing capabilities for field use. This chapter provides an overview of the application of nanotechnology in the field of microbial diagnostics with special focus on plant pathogens.
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Acknowledgments
This work was supported by the Indian Council of Agriculture Research (ICAR) by a network project ‘Application of Microorganisms in Agriculture and Allied Sectors’ (AMAAS).
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Kashyap, P.L. et al. (2016). Nanotechnology for the Detection and Diagnosis of Plant Pathogens. In: Ranjan, S., Dasgupta, N., Lichtfouse, E. (eds) Nanoscience in Food and Agriculture 2. Sustainable Agriculture Reviews, vol 21. Springer, Cham. https://doi.org/10.1007/978-3-319-39306-3_8
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