Abstract
At the beginning of 2016, first generation bioethanol still contributes to the majority of the 25 billion of gallons’ bioethanol produced worldwide, with the United States and Brazil producing approximately 85 % of the global production predominantly based on corn and sugarcane, respectively. However, concerns over the long-term sustainability of first generation bioethanol, such as the impacts on land use, water resource, the potential contamination of soils with the distillation residues, and the competition for food and feed production is frequently highlighted. Current fuel ethanol research and development strives to minimize these negative externalities. The fundamental role that process design plays during the development of cost-effective technologies is evaluated through the modification of the major pathways in first generation ethanol synthesis. In this context, the central role that better performing enzymes and microorganisms play in the intensity and integration of the process, such as the typical example of simultaneous saccharification and fermentation from starchy material in first generation facilities is acknowledged. Compensating ethanol production costs by the integrated valorization of energy and by-products for feed and green chemistry in a typical biorefinery concept are striking outputs of the first generation ethanol real scale experiment. Finally, rather than a mistake, first generation bioethanol should be considered as the first step that made it possible to gain the necessary experience for the successful implementation of the future greener generations biofuels from the field to the tank, starting with second generation lignocellulosic that is now coming on the market. In this context, integrated biorefineries are a promising way to diversify the usable feedstocks, leading to reduced facilities size and optimized supply-chains, to valorize more efficiently bagasse’s from sugarcane and corn stover or even to exploit the potential of microalgae to capture the carbon dioxide that is produced during the fermentation steps. Major stakeholders in bioenergy production are taking advantage of the large-scale successful development of first generation bioethanol, using the most promising processing schemes for next generation facilities, although the industry is still facing uncertainties with respect to its economic viability and longevity.
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Abera S, Rakshit SK (2004) Effect of dry cassava chip storage on yield and functional properties of extracted starch. Starch 56(6):232–240
AFDC (2015) Renewable Fuels Association, Ethanol Industry Outlook 2008-2015 reports. http://www.afdc.energy.gov/data/10331. Accessed 8 Nov 2015
Agrosynergie (2011) Evaluation des measures de la politique agricole commune relatives au secteur du sucre. http://ec.europa.eu/agriculture/eval/…2011/exec_sum_fr.pdf. Accessed 19 Jan 2016
Aiyer PV (2005) Amylases and their applications. Afr J Biotechnol 4(13):1525–1529
Albers E, Larsson C (2009) A comparison of stress tolerance in YPD and industrial lignocellulose-based medium among industrial and laboratory yeast strains. J Ind Microbiol Biotechnol 36(8):1085–1091
Alexandre H, Rousseaux I, Charpentier C (1994) Relationship between ethanol tolerance, lipid composition and plasma membrane fluidity in Saccharomyces cerevisiae and Kloeckera apiculata. FEMS Microbiol Lett 124:17–22
Alfenore S, Cameleyre X, Benbadis L et al (2004) Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process. Appl Microbiol Biotechnol 63(5):537–542
Alvarez MM, Pérez-Carrillo E, Serna-Saldivar SO (2010) Effect of decortication and protease treatment on the kinetics of liquefaction, saccharification, and ethanol production from sorghum. J Chem Technol Biotechnol 85:1122–1130
ANFAVEA (2015) http://www.virapagina.com.br/anfavea2015/. Accessed 08 Nov 2015
Bai FW, Anderson WA, Moo-Young M (2008) Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol Adv 26:89–105
Balat M, Balat H, Cahide OZ (2008) Progress in bioethanol processing. Prog Energ Combust 34:551–573
Baras J, Gacesa S, Pejin D (2002) Ethanol is a strategic raw material. Chem Ind 56:89–10
Basso TO, Gomes FS, Lopes ML et al (2014) Homo- and heterofermentative lactobacilli differentially affect sugarcane-based fuel ethanol fermentation. Ant van Leeuwenhoek Int J Gen Mol Microbiol 105(1):169–177
Benoist A (2009) Eléments d’adaptation de la méthodologie d’analyse de cycle de vie aux carburants végétaux: cas de la première génération. PhD. Ecole Nationale des Mines de Paris, France
Borges EP, Lopes ML, Amorim H (2012) Impact of sugar cane juice chemical composition on clarification and VHP sugar quality. Int Sugar J 114(1364):552–558
Bothast RJ, Schlicher MA (2004) Biotechnological processes for conversion of corn into ethanol. Appl Microbiol Biotechnol 67:19–25
Bvochora JM, Read JS, Zvauya R (2000) Application of very high gravity technology to the cofermentation of sweet stem sorghum juice and sorghum grain. Ind Crop Prod 11:11–17
Carter CA, Miller HI (2012) Corn for food, not fuel. http://www.nytimes.com/2012/07/31/opinion/corn-for-food-not-fuel.html?_r=0. Accessed 08 Nov 2015
Cerqueira Leite RC, Leal MRLVL, Cortez LAB et al (2009) Can Brazil replace 5% of the 2025 gasoline world demand with ethanol? Energy 34:655–661
Cheng JJ (2009) Biological process for ethanol production. Biomass Renew Energy Processes 209–269
Conab (2013) http://www.conab.gov.br/OlalaCMS/uploads/arquivos/13_09_12_17_38_24_5_mandioca_pdf
Congress US, Renewable Fuels, Consumer Protection, and Energy Efficiency Act of 2007 (2007) Section 102, Subtitle A, H.R. 6 (EAS)
Cot M (2006) Etudes physiologiques de l’adaptation et de la résistance de la levure Saccharomyces cerevisiae au cours de la production intensive d’éthanol. PhD. Institut National des Sciences Appliquées de Toulouse
D’Amore T (1992) Improving yeast fermentation performance. J Inst Brew 98:375–382
Das Neves MA, Kimura T, Shimizu N, Shiiba K (2006) Production of alcohol by simultaneous saccharification and fermentation of low-grade wheat flour. Braz Arch Biol Technol 49(3):481–490
Della-Bianca BE, Basso TO, Stambuk BU et al (2013) What do we know about the yeast strains from the Brazillian fuel ethanol industry? Appl Microbiol Biotechnol 97(3):979–991
De Perthuis C, Trotignon R (2015) Le Climat à quel prix?. La Négociation climatique. Editions Odile Jacob, Paris
de Vries SC, van de Ven GWJ, van Ittersum MK et al (2010) Resource use efficiency and environmental performance of nine major biofuel crops, processed by first generation conversion techniques. Biomass Energy 34:588–601
Dutta K, Daverey A, Jih-Gaw Lin J-G (2014) Evolution retrospective for alternative fuels: first to fourth generation. Renew Energy 69:114–122
Echegaray O, Carvalho J, Fernandes A et al (2000) Fed-batch culture of Saccharomyces cerevisiae in sugarcane blackstrap molasses: invertase activity of intact cells in ethanol fermentation. Biomass Bioenergy 19:39–50
EIA (2013) http://www.eia.gov/todayinenergy/detail.cfm?id=11551. Accessed 08 Nov
European Commission (2010) Energy 2020: a strategy for competitive, sustainable and secure energy. COM (2010) 639 final, Brussels
European Parliament (2010) A new energy strategy for Europe 2011-2020. P7_TA (2010)0441, Brussels
Eurostat (2011) Statistical data. http://epp.eurostat.ec.europa.eu/portal/page/portal/eurostat/home/S
Gnansounou E, Dauriat A, Wyman CE (2005) Refining sweet sorghum to ethanol and sugar: economic trade-offs in the context of North China. Biores Technol 96(9):985–1002
Gonçalves FA, dos Santos ES, de Macedo GR (2015) Alcoholic fermentation of Saccharomyces cerevisiae, Pichia stipitis and Zymomonas mobilis in the presence of inhibitory compounds and seawater: Fermentation in the presence of inhibitors. J Basic Microbiol 55(6):695–708
Gollier C, Tirole J (2015) Negotiating effective institutions against climate change. Econ Energy Environ Pol 4(2):5–28
Graham-Rowe D (2011) Beyond food versus fuel. Nature 474:S6e8
Gray JV, Petsko GA, Johnston GC et al (2004) “Sleeping beauty”: quiescence in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 68:187–206
Gupta A, Verma JP (2015) Sustainable bio-ethanol production from agro-residues: A review. Renew Sust Energy Rev 41:550–567
Haankuku C, Epplin FM, Kakani VG (2015) Industrial sugar beets to biofuel: field to fuel production system and cost estimates. Biomass Bioen 80:267–277
Harris PV, Wogulis M (2010) Polypeptides having amylolytic enhancing activity and polynucleotides encoding the same. Patent No. WO/2010/059413
Harris PV, Xu F, Kreel NE et al (2014) New enzyme insights drive advances in commercial ethanol production. Curr Opin Chem Biol 19:162–170
Herman PK (2002) Stationary phase in yeast. Curr Opin Microbiol 5:602–607
Hira A, Oliveira LG (2009) No substitute for oil? How Brazil developed its ethanol industry. Energ Policy 37:2450–2456
Horn SV, Vaaje-Kolstad G, Westereng B et al (2012) Novel enzymes for the degradation of cellulose. Biotechnol Biofuels 5:45
IBGE (2008) Brazilian Institute of Geography and Statistics [Instituto Brasileiro de Geografia e Estatística—IBGE]. Evolução da Produtividade da Cana-de-Açúcar no Brasil. www.ibge.gov.brS
Ishikawa K, Nakatani H, Katsuya Y et al (2007) Kinetic and structural analysis of enzyme sliding on a substrate: multiple attack in β-amylase. Biochem 46:792–798
Johnston DB, McAloon AJ (2014) Protease increases fermentation rate and ethanol yield in dry-grind ethanol production. Biores Technol 154:18–25
Kang Y-N, Tanabe A, Adachi M et al (2005) Structural analysis of threonine 342 mutants of soybean b-amylase: role of a conformational change of the inner loop in the catalytic mechanism. Biochem 44:5106–5116
Kim I-S, Moon H-Y, Yun H-S et al (2006) Heat shock causes oxidative stress and induces a variety of cell rescue proteins in Saccharomyces cerevisiae KNU5377. J Microbiol (Seoul, Korea) 44(5):492–501
Koppram R, Olsson L (2014) Combined substrate, enzyme and yeast feed in simultaneous saccharification and fermentation allow bioethanol production from pretreated spruce biomass at high solids loading. Biotechnol Biofuel 7. doi:10.1186/1754-6834-7-54
Kramer GFH, Gunning AP, Morris VJ et al (1993) Scanning tunneling microscopy of Aspergillus niger glucoamylase. J Chem Soc, Faraday Trans 89:2595–2602
Koh LP, Ghazoul J (2008) Biofuels, biodiversity, and people: Understanding the conflicts and finding opportunities. Biol Conserv 141(10):2450–2460
Larkin S, Ramage J, Scurlock J (2004). Bioenergy. In: Boyle G (ed) Renewable energy. Oxford University Press, p 135
Lei H, Zheng L, Wang C, Zhao H et al (2013) Effects of worts treated with proteases on the assimilation of free amino acids and fermentation performance of lager yeast. Int J Food Microbiol 161:76–83
Lennartsson PR, Erlandsson P, Taherzadeh MJ (2014) Integration of the first and second generation bioethanol process and the importance of by-products. Biores Technol 165:3–8
Linoj KNV, Dhavala P, Goswami A et al (2006) Liquid biofuels in South Asia: resources and technologies. Asian Biotechnol Develop Rev 8:31–49
Lo Leggio L, Simmons TJ, Poulsen J-CN et al (2015) Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase. Nature Comms 6:5961
Lombard V, Ramulu HG, Drula E et al (2014) The carbohydrate-active enzymes databases (CAZy) in 2013. Nucleic Acids Res 42:490–495
Luckett CR, Wang Y-J (2012) Effects of β-amylolysis on the resistant starch formation of debranched corn starches. J Agric Food Chem 60:4751–4757
Lundgard R, Svensson B (1987) The four major forms of barley a-amylase. Purification, characterization and structural relationship. Carlsberg Res Commun 52:313–326
Macrelli S, Galbe M, Wallberg O (2014) Effects of production and market factors on ethanol profitability for an integrated first and second generation ethanol plant using the whole sugarcane as feedstock. Biotech Biofuels 7:26
Maity JP, Bundschuh J, Chen C-Y et al (2014) Microalgae for third generation biofuel production, mitigation of greenhouse gas emissions and wastewater treatment: present and future perspectives—a mini review. Energy 78:104–113
Maity SK (2015a) Opportunities, recent trends and challenges of integrated biorefinery: part I. Renew Sustain Energy Rev 43:1427–1445
Maity SK (2015b) Opportunities, recent trends and challenges of integrated biorefinery: Part II. Renew Sustain Energy Rev 43:1446–1466
Maranduba HL, Robra S, Nascimento IA et al (2015) Reducing the life cycle GHG emissions of microalgal biodiesel through integration with ethanol production system. Biores Technol 194:21–27
McAloon A, Taylor F, Yee W et al (2000) Determining the cost of producing ethanol from corn starch and lignocellulosic feedstocks. http://www.nrel.gov/docs/fy01osti/28893.pdf. Accessed 08 Nov 2015
Mikami B, Iwamoto H, Malle D et al (2006) Crystal structure of pullulanase: evidence for parallel binding of oligosaccharides in the active site. J Mol Biol 359:690–707
Morano KA, Liu PC, Thiele DJ (1998) Protein chaperones and the heat shock response in Saccharomyces cerevisiae. Curr Opin Microbiol 1(2):197–203
Morris VJ, Gunning AP, Faulds CB et al (2005) AFM images of complexes between amylose and Aspergillus niger glucoamylase mutants, native and mutant starch binding domains: a model for the action of glucoamylase. Starch 57:1–7
Mussatto SI, Roberto IC (2004) Alternatives for detoxification of diluted-acid lignocellulosic hydrolyzates for use in fermentative processes: a review. Biores Technol 93:1–10
Nielsen PK, Bønsager BC, Fukuda K et al (2004) Barley a-amylase/subtilisin inhibitor: structure, biophysics and protein engineering. Biochim Biophys Acta 1696:157–164
Nikolov ZL, Meagher MM, Reilly PJ (1989) Kinetics, equilibria, and modeling of the formation of oligosaccharides from d-glucose by Aspergillus niger glucoamylase. Biotechnol Bioeng 34:694–704
NREL (2014) Renewable Energy Data Book, US Dept of Energy. www.nrel.gov/docs/fy16osti/64720.pdf. Accessed 19 Jan 2016
OECD/IEA (2008) From 1st to 2nd generations of biofuel technologies, overview of current industry and RD&D activities
Ostanin K, Harms EH, Stevis PE (1992) Overexpression, site directed mutagenesis and mechanism of Escherichia coli acid phosphatase. J Biol Chem 267:22830–22836
Pagliardini J (2010) Optimisation du rendement de production de bioéthanol chez Saccharomyces cerevisiae par minimisation de la synthèse du glycérol: approche intégrée de génie métabolique et microbiologique. PhD. Toulouse University, France
Pereira LFB (2014) Bioethanol—robust production strains for process identification. PhD. Univesidade do Minho, Portugal
Pipper PW (1995) The heat-shock and ethanol stress responses of yeast exhibit extensive similarity and functional overlap. FEMS Yeast Res 134(2–3):121–127
Pizarro FJ, Jewett MC, Nielsen J et al (2008) Growth temperature exerts differential physiological and transcriptional responses in laboratory and wine strains of Saccharomyces cerevisiae. Appl Environ Microbiol 74(20):6358–6368
Postmus J (2011) The physiological response of Saccharomyces cerevisiae to temperature stress. PhD. Amsterdam University, The Netherlands
Puligundla P, Smogrovicova D, Obulam VSR, Ko S (2011) Very high gravity (VHG) ethanolic brewing and fermentation: a research update. J Ind Microbiol Biotechnol 38:1133–1144
Quintero JA, Montoya MI, Sánchez OJ et al (2008) Fuel ethanol production from sugarcane and corn: comparative analysis for a Colombian case. Energy 33:385–399
Reiss J (2012) Intensification de la brique «fermentation alcoolique» de substrats betteraviers pour la production d’éthanol. PhD. Toulouse University, France
REN21 (2012) Renewables 2012? Global Status Report, REN21 Secretariat, Paris
Rockey WM, Laederach A, Reilly PJ (2000) Automated docking of α-(1→4)- and α-(1→6)-linked glucosyl trisaccharides and maltopentaose into the soybean β-amylase active site. Proteins Struct Funct Genet 40:299–309
Rojanaridpiched C, Kosintarasaenee S, Sriroth K et al (2003) Development of Ethanol Production Technology from Cassava Chip at a Pilot Plant Scale. National Research Council of Thailand
Rosillo-Calle F, Cortez LAB (1998) Towards ProAlcool II—a review of the Brazilian bioethanol programme. Biomass Bioenergy 14:115–124
Ruan Q, Chen WB, Huang SH (2001) The mathematics model and matrix method of complex cocurrent multi-effect evaporation. Eng Sci 3:36–41
Salazar-Ordóñez M, Pérez-Hernández PP, Martín-Lozano JM (2013) Sugar beet for bioethanol production: an approach based on environmental agricultural outputs. Energy Policy 55:662–668
Sanchez OJ, Cardona CA (2008) Trends in biotechnological production of fuel ethanol from different feedstocks. Biores Technol 99:5270–5295
Sancho AI, Faulds CB, Svensson B et al (2003) Cross-inhibitory activity of cereal protein inhibitors against a-amylases and xylanases. Biochim Biophys Acta 1650:136–144
Seabra JEA, Macedo IC, Chum HL et al (2011) Life cycle assessment of Brazilian sugarcane products: GHG emissions and energy use. Biofuels Bioprod Biorefin 5:519–532
Siqueira PF, Karp SG, Carvalho JC et al (2008) Production of bio-ethanol from soybean molasses by Saccharomyces cerevisiae at laboratory, pilot and industrial scales. Biores Technol 99:8156–8163
Smeets E, Tabeau A, van Berkum S et al (2014) The impact of the rebound effect of the use of first generation biofuels in the EU on greenhouse gas emissions: a critical review. Renew Sustain Energy Rev 38:393–403
Snoek T, Picca Nicolino M, Van den Bremt S et al (2015) Large-scale robot-assisted genome shuffling yields industrial Saccharomyces cerevisiae yeasts with increased ethanol tolerance. Biotech Biofuels 8(1):32
Soccol CR, Vandenberghe LPS, Costa B et al (2005) Brazilian biofuel program: an overview. J Sci Ind Res 64:897–904
Soccol CR, Vandenberghe LPD, Medeiros ABP et al (2010) Bioethanol from lignocelluloses: status and perspectives in Brazil. Biores Technol 101(13):4820–4825
Souza SP, Gopal AR, Seabra JEA (2015) Life cycle assessment of biofuels from an integrated Brazilian algae-sugarcane biorefinery. Energy 81:373–381
Sriroth K, Wanlapatit S, Piyachomkwan K (2012) Cassava Bioethanol, Bioethanol, Prof. Marco Aurelio Pinheiro Lima (Ed.), ISBN: 978-953-51-0008-9, InTech. http://www.intechopen.com/books/bioethanol/-cassava-bioethanol
Steensels J, Snoek T, Meersman E et al (2014) Improving industrial yeast strains: exploiting natural and artificial diversity. FEMS Microbiol Rev 38(5):947–995
Suutari M, Liukkonen K, Laakso S (1990) Temperature adaptation in yeasts: the role of fatty acids. J Gen Microbiol 136(8):1469–1474
Thomas KC, Ingledew WM (1992) Production of 21% (v/v) ethanol by fermentation of very high gravity (VHG) wheat mashes. J Ind Microbiol 10(1):61–68
Trivedi N, Gupta V, Reddy C et al (2015) Marine macroalgal biomass as a renewable source of bioethanol. In: Kim S-K, Lee C-G (eds) Marine Bioenergy. CRC Press, pp 197–216
Udop (2015) União dos Produtores de Bioenergia http://www.udop.com.br/index.php?item=safras. Accessed 08 Nov 2015
Unica (2015) União da Indústria da Cana de Açúcar. http://www.unicadata.com.br/historico-de-producao-e-moagem.php. Accessed 08 Nov 2015
UNCTAD (2015) Commodity profile—Cassava. http://www.unctad.info/en/Infocomm/AACP-Products/COMMODIRY-PROFILE---Cassava/. Accessed 08 Nov 2015
Vidal BC Jr, Rausch KD, Tumbleson M et al (2009) Protease treatment to improve ethanol fermentation in modified dry gring corn processes. Cereal Chem 86:323–328
Walker-Caprioglio HM, Casey WM, Parks LW (1990) Saccharomyces cerevisiae membrane sterol modifications in response to growth in the presence of ethanol. Appl Environ Microbiol 56:2853–2857
Wang P, Johnston DB, Rausch KD et al (2009) Effects of protease and urea on a granular starch hydrolyzing process for corn ethanol production. Cereal Chem 86:319–322
Wang L, Raul Quiceno R, Price C et al (2014) Economic and GHG emissions analyses for sugarcane ethanol in Brazil: looking forward. Renew Sustain Energy Rev 40:571–582
Wheals AE, Basso LC, Alves DMG et al (1999) Fuel ethanol after 25 years. Trends Biotechnol 17(12):482–487
Woods J (2000) Integrating sweet sorghum and sugarcane for bioenergy: modelling the potential for electricity and ethanol production in SE Zimbabwe. PhD thesis. King’s College, London
Woods J (2001) The potential for energy production using sweet sorghum in southern Africa. Energy Sustain Dev 1:31–38
Yoosin S, Sorapipatana C (2007) A study of ethanol production cost for gasoline substitution in Thailand and its competitiveness: thammasat. Int J Sci Technol 12:69–80
Zarrilli S (2006) The emerging biofuels market: regulatory, trade and development implications. UNCTAD Intergovernmental Expert Meeting on BioFuels, Geneva, November 3 2006
Zhou GT, Jiang ZM, Dong XL et al (2011) Recycling and refining of alcohol derived from waste beer separated from spent yeast. J Am Soc Brew Chem 69(3):158–162
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Bertrand, E., Vandenberghe, L.P.S., Soccol, C.R., Sigoillot, JC., Faulds, C. (2016). First Generation Bioethanol. In: Soccol, C., Brar, S., Faulds, C., Ramos, L. (eds) Green Fuels Technology. Green Energy and Technology. Springer, Cham. https://doi.org/10.1007/978-3-319-30205-8_8
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