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Prokaryotic Systems Biology pp 135–154Cite as

Quantitative and Systems-Based Approaches for Deciphering Bacterial Membrane Interactome and Gene Function

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 883))

Abstract

High-throughput genomic and proteomic methods provide a concise description of the molecular constituents of a cell, whereas systems biology strives to understand the way these components function as a whole. Recent developments, such as genome editing technologies and protein epitope-tagging coupled with high-sensitivity mass-spectrometry, allow systemic studies to be performed at an unprecedented scale. Available methods can be successfully applied to various goals, both expanding fundamental knowledge and solving applied problems. In this review, we discuss the present state and future of bacterial cell envelope interactomics, with a specific focus on host–pathogen interactions and drug target discovery. Both experimental and computational methods will be outlined together with examples of their practical implementation.

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Acknowledgements

This work was supported in part by funds from the Natural Sciences and Engineering Research Council of Canada (DG-20234) to MB. JV was supported by a Saskatchewan Postdoctoral Research Fellowship. MB holds a Canadian Institute of Health Research New Investigator award.

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Authors and Affiliations

  1. Department of Biochemistry, Research and Innovation Centre, University of Regina, Regina, SK, Canada

    Viktor Deineko, Ashwani Kumar, James Vlasblom & Mohan Babu

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  1. Viktor Deineko
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  2. Ashwani Kumar
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  3. James Vlasblom
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  4. Mohan Babu
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Correspondence to Mohan Babu .

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Editors and Affiliations

  1. Cellular and Molecular Pharmacology, Univ of California, San Francisco, SAN FRANCISCO, California, USA

    Nevan J. Krogan, PhD

  2. Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada

    Mohan Babu, PhD

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Deineko, V., Kumar, A., Vlasblom, J., Babu, M. (2015). Quantitative and Systems-Based Approaches for Deciphering Bacterial Membrane Interactome and Gene Function. In: Krogan, PhD, N., Babu, PhD, M. (eds) Prokaryotic Systems Biology. Advances in Experimental Medicine and Biology, vol 883. Springer, Cham. https://doi.org/10.1007/978-3-319-23603-2_8

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