Abstract
Next-generation sequencing (NGS) has revolutionized the field of genomics since the release of the first NGS sequencer in 2005. For the last 10 years, the second generation of sequencing machines has reached tremendous throughput and accuracy that enables researchers to conduct large-scale genomic projects at ever-decreasing cost and time. It is now commonplace to sequence the entire genome of individuals. This is ushering in a new era in genomic medicine, allowing characterizing the genome of patients and tumor cells to uncover disease-causing mutations, information that can be used to inform about the best therapeutic options. High-throughput sequencing enables researchers to complete large-scale experiments in human cells and model organisms for genome, exome, transcriptome, and small RNA analyses, localization of protein binding, and epigenetics marks. Next-generation sequencing aims to become the new gold standard for scientific research as well as for clinical genetic testing.
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Frio, T.R. (2015). High-Throughput Technologies: DNA and RNA Sequencing Strategies and Potential. In: Le Tourneau, C., Kamal, M. (eds) Pan-cancer Integrative Molecular Portrait Towards a New Paradigm in Precision Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-22189-2_5
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DOI: https://doi.org/10.1007/978-3-319-22189-2_5
Publisher Name: Springer, Cham
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