Abstract
Optical recording with fast voltage sensitive dyes makes it possible, in suitable preparations, to simultaneously monitor the action potentials of large numbers of individual neurons. Here we describe methods for doing this, including considerations of different dyes and imaging systems, methods for correlating the optical signals with their source neurons, procedures for getting good signals, and the use of Independent Component Analysis for spike-sorting raw optical data into single neuron traces. These combined tools represent a powerful approach for large-scale recording of neural networks with high temporal and spatial resolution.
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References
Alivisatos AP, Chun M et al (2013) Neuroscience: the brain activity map. Science 339:1284–1285
Bell AJ, Sejnowski TJ (1995) An information-maximization approach to blind separation and blind deconvolution. Neural Comput 7(6):1129–1159
Boyle MB, Cohen LB et al (1983) The number and size of neurons in the CNS of gastropod molluscs and their suitability for optical recording of activity. Brain Res 266(2):305–317
Briggman KL, Abarbanel HD et al (2006) From crawling to cognition: analyzing the dynamical interactions among populations of neurons. Curr Opin Neurobiol 16(2):135–144
Brown GD, Yamada S et al (2008) Independent component analysis of optical recordings from Tritonia swimming neurons, Technical Report INC-08-001, Institute for Neural Computation, University of California at San Diego
Brown GD, Yamada S et al (2001) Independent component analysis at the neural cocktail party. Trends Neurosci 24(1):54–63
Bruno AM, Frost WN et al (2015) Modular deconstruction reveals the dynamical and physical building blocks of a locomotion motor program. Neuron 86:304–18
Carlson GC, Coulter DA (2008) In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording. Nat Protoc 3(2):249–255
Chang PY, Jackson MB (2003) Interpretation and optimization of absorbance and fluorescence signals from voltage-sensitive dyes. J Membr Biol 196(2):105–116
Cohen L, Hopp HP et al (1989) Optical measurement of action potential activity in invertebrate ganglia. Annu Rev Physiol 51:527–541
Delorme A, Makeig S (2004) EEGLAB: an open source toolbox for analysis of single-trial EEG dynamics including independent component analysis. J Neurosci Methods 134(1):9–21
Ebner TJ, Chen G (1995) Use of voltage-sensitive dyes and optical recordings in the central nervous system. Prog Neurobiol 46(5):463–506
Feldt S, Waddell J et al (2009) Functional clustering algorithm for the analysis of dynamic network data. Phys Rev E Stat Nonlin Soft Matter Phys 79(5 Pt 2):056104
Frost WN, Wang J et al (2007) A stereo-compound hybrid microscope for combined intracellular and optical recording of invertebrate neural network activity. J Neurosci Methods 162(1–2):148–154
Gerstein GL, Williams ER et al (2012) Detecting synfire chains in parallel spike data. J Neurosci Methods 206(1):54–64
Grinvald A, Salzberg BM et al (1977) Simultaneous recording from several neurones in an invertebrate central nervous system. Nature 268(5616):140–142
Hill ES, Bruno AM et al (2014) Recent developments in VSD imaging of small neuronal networks. Learn Mem 21(10):499–505
Hill ES, Bruno AM et al (2012) ICA applied to VSD imaging of invertbrate neuronal networks. In: Ganesh N (ed) Independent component analysis for audio and biosignal applications. InTech: Chapter 12, p. 235–246
Hill ES, Moore-Kochlacs C et al (2010) Validation of independent component analysis for rapid spike sorting of optical recording data. J Neurophysiol 104(6):3721–3731
Humphries MD (2011) Spike-train communities: finding groups of similar spike trains. J Neurosci 31(6):2321–2336
Jin W, Zhang RJ et al (2002) Voltage-sensitive dye imaging of population neuronal activity in cortical tissue. J Neurosci Methods 115(1):13–27
Koch C (2012) Modular biological complexity. Science 337(6094):531–532
Kojima S, Hosono T et al (2001) Optical detection of neuromodulatory effects of conditioned taste aversion in the pond snail Lymnaea stagnalis. J Neurobiol 49(2):118–128
Kosmidis EK, Cohen LB et al (2005) Imaging with voltage-sensitive dyes: spike signals, population signals, and retrograde transport. In: Yuste R, Konnerth A (eds) Imaging in neuroscience and development: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 445–455
London JA, Zecevic D et al (1987) Simultaneous optical recording of activity from many neurons during feeding in Navanax. J Neurosci 7(3):649–661
Lopes-dos-Santos V, Conde-Ocazionez S et al (2011) Neuronal assembly detection and cell membership specification by principal component analysis. PLoS One 6(6), e20996
Lyttle D, Fellous JM (2011) A new similarity measure for spike trains: sensitivity to bursts and periods of inhibition. J Neurosci Methods 199(2):296–309
Miller EW, Lin JY et al (2012) Optically monitoring voltage in neurons by photo-induced electron transfer through molecular wires. Proc Natl Acad Sci U S A 109(6):2114–2119
Momose-Sato Y, Sato K et al (1999) Evaluation of voltage-sensitive dyes for long-term recording of neural activity in the hippocampus. J Membr Biol 172(2):145–157
Nakashima M, Yamada S et al (1992) 448-Detector optical recording system: development and application to Aplysia gill-withdrawal reflex. IEEE Trans Biomed Eng 39:26–36
Neunlist M, Peters S et al (1999) Multisite optical recording of excitability in the enteric nervous system. Neurogastroenterol Motil 11(5):393–402
Nikitin ES, Balaban PM (2000) Optical recording of odor-evoked responses in the olfactory brain of the naive and aversively trained terrestrial snails. Learn Mem 7(6):422–432
Obaid AL, Koyano T et al (1999) Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus. J Neurosci 19(8):3073–3093
Obaid AL, Loew LM et al (2004) Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis. J Neurosci Methods 134(2):179–190
Parsons TD, Salzberg BM et al (1991) Long-term optical recording of patterns of electrical activity in ensembles of cultured Aplysia neurons. J Neurophysiol 66:316–333
Salzberg BM, Grinvald A et al (1977) Optical recording of neuronal activity in an invertebrate central nervous system: simultaneous monitoring of several neurons. J Neurophysiol 40(6):1281–1291
Schemann M, Michel K et al (2002) Cutting-edge technology. III. Imaging and the gastrointestinal tract: mapping the human enteric nervous system. Am J Physiol Gastrointest Liver Physiol 282(6):G919–G925
Senseman DM (1996) High-speed optical imaging of afferent flow through rat olfactory bulb slices: voltage-sensitive dye signals reveal periglomerular cell activity. J Neurosci 16(1):313–324
Sinha SR, Saggau P (1999) Optical recording from populations of neurons in brain slices. In: Johanson H, Windhorst U (eds) Modern techniques in neuroscience research. p. 459–486
Taylor AL, Cottrell GW et al (2003) Imaging reveals synaptic targets of a swim-terminating neuron in the leech CNS. J Neurosci 23(36):11402–11410
Traud AL, Frost C et al (2009) Visualization of communities in networks. Chaos 19(4):041104
Vanden Berghe P, Bisschops R et al (2001) Imaging of neuronal activity in the gut. Curr Opin Pharmacol 1(6):563–567
Wang Y, Jing G et al (2009) Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons. Opt Express 17(2):984–990
Wu J, Cohen LB et al (1994) Neuronal activity during different behaviors in Aplysia: a distributed organization. Science 263:820–823
Yagodin S, Collin C et al (1999) Mapping membrane potential transients in crayfish (Procambarus clarkii) optic lobe neuropils with voltage-sensitive dyes. J Neurophysiol 81(1):334–344
Yang S, Doi T et al (2000) Multiple-site optical recording of mouse brainstem evoked by vestibulocochlear nerve stimulation. Brain Res 877(1):95–100
Yuste R (2008) Circuit neuroscience: the road ahead. Front Neurosci 2(1):6–9
Zecevic D, Wu J et al (1989) Hundreds of neurons in the Aplysia abdominal ganglion are active during the gill-withdrawal reflex. J Neurosci 9:3681–3689
Zochowski M, Cohen LB et al (2000a) Distributed and partially separate pools of neurons are correlated with two different components of the gill-withdrawal reflex in Aplysia. J Neurosci 20(22):8485–8492
Zochowski M, Wachowiak M et al (2000b) Imaging membrane potential with voltage-sensitive dyes. Biol Bull 198(1):1–21
Acknowledgments
Supported by NIH NS060921, NSF 1257923, Dart Foundation and Grass Foundation Marine Biological Laboratory summer fellowships, the Fred B. Snite Foundation, Rosalind Franklin University of Medicine and Science (WF), NIH F31NS079036 (AB), MRC Senior non-Clinical Fellowship (MH) and the Howard Hughes Medical Institute (TS).
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Frost, W.N. et al. (2015). Monitoring Spiking Activity of Many Individual Neurons in Invertebrate Ganglia. In: Canepari, M., Zecevic, D., Bernus, O. (eds) Membrane Potential Imaging in the Nervous System and Heart. Advances in Experimental Medicine and Biology, vol 859. Springer, Cham. https://doi.org/10.1007/978-3-319-17641-3_5
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DOI: https://doi.org/10.1007/978-3-319-17641-3_5
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