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Second Harmonic OCT and Combined MPM/OCT

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Optical Coherence Tomography

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Abstract

This chapter describes combined multiphoton microscopy (MPM) and optical coherence tomography (OCT) system and second harmonic OCT (SH-OCT) system. Second harmonic generation (SHG) enables direct imaging of optically anisotropic biological structures, such as membranes, structural proteins, microtubule ensembles, and collagen. SH-OCT combines molecular contrast of SHG with coherence gating of OCT. MPM/OCT combines molecular contrast of MPM with scattering contrast of OCT. Combining MPM and OCT onto a single platform creates a novel multimodality image technique which can acquire structural and functional imaging of tissues simultaneously. We will review the principle and technology of SH-OCT and combined MPM/OCT system, and illustrate a few examples of its applications.

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Acknowledgments

We would like to thank many of our colleagues who have contributed to the MPM/OCT and SH-OCT projects at UCI and UBC. We want to acknowledge grant support from the National Institutes of Health (R01EB-00293, R01CA-91717, R01EB-10090, R01EY-021519, R01HL-105215, P41EB-015890), Air Force Office of Scientific Research (F49620-00-1-0371), the Beckman Laser Institute Endowment, Natural Sciences and Engineering Research Council of Canada, and the Canada Foundation for Innovation.

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Authors and Affiliations

  1. The Edwards Life Sciences Center for Advanced Cardiovascular Technology, Beckman Laser Institute, Irvine, CA, 92612, USA

    Zhongping Chen

  2. Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Shuo Tang

  3. Department of Biomedical Engineering, Beckman Laser Institute, University of California Irvine, Irvine, CA, 92612, USA

    Zhongping Chen

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  1. Zhongping Chen
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Correspondence to Zhongping Chen .

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Editors and Affiliations

  1. Center for Medical Physics and Biomedical Engineering, Medical University Vienna, General Hospital Vienna, Vienna, Austria

    Wolfgang Drexler

  2. Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

    James G. Fujimoto

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Chen, Z., Tang, S. (2015). Second Harmonic OCT and Combined MPM/OCT. In: Drexler, W., Fujimoto, J. (eds) Optical Coherence Tomography. Springer, Cham. https://doi.org/10.1007/978-3-319-06419-2_50

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