Abstract
Biodiesel production from microalgae lipids showed to be the promise approach for alternative fuels. However, this is not yet commercialized. In the present study, the efficient production of biodiesel from Nannochlorpsis sp. grown locally was confirmed. Novozyme®435 and supercritical carbon dioxide were used as reaction catalyst and reaction medium, respectively, and the results were compared to lamb fat. In addition, the microalgae biomass production was studied extensively. It was found that Nannochlorpsis sp. can accumulate lipids by nitrogen starvation reaching 29 % with 21.06 mg L-1d-1 lipid productivity when grown in nitrogen sufficient medium. The enzymatic production in supercritical carbon dioxide was efficient with 55 % ester yield higher than lamb meat fat.
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References
D. Rutz, R. Janssen, in BioFuel Technology Handbook: WIP Renewable Energies. (Elsevier Biomedical Press, Amsterdam, 2007)
S. Al-Zuhair, Production of biodiesel: possibilities and challenges. Biofuels, Bioprod. Biorefin. 1, 57–66 (2007)
H.N. Bhatti, M.A. Hanif, M. Qasim, R. Ata ur, Biodiesel production from waste tallow. Fuel 87, 2961–2966 (2008)
M.K. Lam, K.T. Lee, A.R. Mohamed, Sulfated tin oxide as solid superacid catalyst for transesterification of waste cooking oil: an optimization study. Appl. Catal. B 93, 134–139 (2009)
M.G. De Paola, E. Ricca, V. Calabr, S. Curcio, G. Iorio, Factor analysis of transesterification reaction of waste oil for biodiesel production. Bioresour. Technol. 100, 5126–5131 (2009)
F. Ma, L.D. Clements, M.A. Hanna, Biodiesel fuel from animal fat. Ancillary studies on transesterification of beef tallow. Ind. Eng. Chem. Res. 37, 3768–3771 (1998)
H. Taher, S. Al-Zuhair, A. AlMarzouqui, I. Hashim, Extracted fat from lamb meat by supercritical CO2 as feedstock for biodiesel production. Biochem. Eng. J. 55, 23–31 (2011)
H. Taher, S. Al-Zuhair, A.H. Al-Marzouqi, Y. Haik, M.M. Farid, A review of enzymatic transesterification of microalgal oil-based Biodiesel using supercritical technology. Enzyme Res. 468292, 1–25 (2011)
V. Patil, K.-Q. Tran, H.R. Giselrd, Towards sustainable production of biofuels from microalgae. Int. J. Mol. Sci. 9, 1188–1195 (2008)
N. Moazami, A. Ashori, R. Ranjbar, M. Tangestani, R. Eghtesadi, A.S. Nejad, Large-scale biodiesel production using microalgae biomass of nannochloropsis. Biomass Bioenergy 39, 449–453 (2012)
Y. Chisti, Biodiesel from microalgae. Biotechnol. Adv. 25, 294–306 (2007)
M. Adamczak, U.T. Bornscheuer, W. Bednarski, The application of biotechnological methods for the synthesis of biodiesel. Eur. J. Lipid Sci. Technol. 111, 800–813 (2009)
P. Spolaore, C. Joannis-Cassan, E. Duran, A.n. Isambert, Commercial applications of microalgae. J. Biosci. Bioeng. 101, 87–96 (2006)
L. Rodolfi, G.C. Zittelli, N. Bassi, G. Padovani, N. Biondi, G. Bonini, M.R. Tredici, Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol. Bioeng. 102, 100–112 (2009)
L. Gouveia, A.C. Oliveira, Microalgae as a raw material for biofuels production. J. Ind. Microbiol. Biotechnol. 36, 269–274 (2009)
S.Y. Chiu, C.Y. Kao, M.T. Tsai, S.C. Ong, C.H. Chen, C.S. Lin, Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresour. Technol. 100, 833–838 (2009)
E.G. Bligh, W.J. Dyer, A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917 (1959)
J. Folch, M. Lees, G.H. Sloane Stanley, A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509 (1957)
A. Hara, N.S. Radin, Lipid extraction of tissues with a low-toxicity solvent. Anal. Biochem. 90, 420–426 (1978)
R.L. Mendes, B.P. Nobre, M.T. Cardoso, A.P. Pereira, A.n.F. Palavra, Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae. Inorg. Chim. Acta 356, 328–334 (2003)
J.P. Friedrich, E.H. Pryde, Supercritical CO2 extraction of lipid-bearing materials and characterization of the products. J. Am. Oil Chem. Soc. 61, 223–228 (1984)
P. Tonthubthimthong, P.L. Douglas, S. Douglas, W. Luewisutthichat, W. Teppaitoon, L.-E. Pengsopa, Extraction of nimbin from neem seeds using supercritical CO2 and a supercritical CO2—methanol mixture. J. Supercrit. Fluids 30, 287–301 (2004)
G. Andrich, U. Nesti, F. Venturi, A. Zinnai, R. Fiorentini, Supercritical fluid extraction of bioactive lipids from the microalga Nannochloropsis sp. Eur. J. Lipid Sci. Technol. 107, 381–386 (2005)
K.P. Dhake, K.M. Deshmukh, Y.P. Patil, R.S. Singhal, B.M. Bhanage, Improved activity and stability of Rhizopus oryzae lipase via immobilization for citronellol ester synthesis in supercritical carbon dioxide. J. Biotechnol. 156, 46–51 (2011)
W. Xiong, X. Li, J. Xiang, Q. Wu, High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl. Microbiol. Biotechnol. 78, 29–36 (2008)
X. Li, H. Xu, Q. Wu, Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors. Biotechnol. Bioeng. 98, 764–771 (2007)
O.K. Lee, Y.H. Kim, J.-G. Na, Y.-K. Oh, E.Y. Lee, Highly efficient extraction and lipase-catalyzed transesterification of triglycerides from Chlorella sp. KR-1 for production of biodiesel. Bioresour. Technol. 147, 240–245 (2013)
S. Anitha, J. Narayanan, Biodiesel production from chlorella vulgaris with special emphasis on immobilized lipase catalyzed transesterification. Int. J. Recent Sci. Res. 3, 733–737 (2012)
D.C. Rule, Direct transesterification of total fatty acids of adipose tissue, and of freeze-dried muscle and liver with boron-trifluoride in methanol. Meat Sci. 46, 23–32 (1997)
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Taher, H., Al-Zuhair, S., Al-Marzouqi, A., Haik, Y., Farid, M. (2014). Effectiveness of Enzymatic Biodiesel Production from Microalga Oil in Supercritical Carbon Dioxide. In: Hamdan, M., Hejase, H., Noura, H., Fardoun, A. (eds) ICREGA’14 - Renewable Energy: Generation and Applications. Springer Proceedings in Energy. Springer, Cham. https://doi.org/10.1007/978-3-319-05708-8_5
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