Skip to main content

A More in Depth Look at the EPR Signal Response

  • Chapter
  • First Online:
Quantitative EPR

Abstract

Optimizing an EPR experiment depends on sample preparation, an understanding of the interaction of the sample with the spectrometer, and the selection of appropriate operating parameters.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Weil, J.E., Bolton, J.R., Wertz, J.A.: Electron Paramagnetic Resonance: Elementary Theory and Practical Applications, p. 497. Wiley, New York (1994a)

    Google Scholar 

  • Aasa, R., Vanngard, T.: EPR signal intensity and powder shapes. Reexamination. J. Magn. Reson. 19, 308–315 (1975)

    Google Scholar 

  • Anderson, J.R., Mett, R.R., Hyde, J.S.: Cavities with axially uniform fields for use in electron paramagnetic resonance. II. Free space generalization. Rev. Sci. Instrum. 73(8), 3027–3037 (2002)

    Article  ADS  Google Scholar 

  • Beth, A., Balasubramanian, K., Robinson, B.H., Dalton, L.R., Venkataramu, S.K., Park, J.H.: Sensitivity of V2' saturation transfer electron paramagnetic resonance signals to anisotropic rotational diffusion with [15N]nitroxide spin-labels. Effects of noncoincident magnetic and diffusion tensor principal axes. J. Phys. Chem. 87, 359–367 (1983)

    Article  Google Scholar 

  • Burgess, J.H., Brown, R.M.: Modulation effects in nuclear magnetic resonance. Rev. Sci. Instrum. 23, 334–336 (1952)

    Article  ADS  Google Scholar 

  • Calvo, R., Abresch, E.C., Bittl, R., Feher, G., Hofbauer, W., Isaacson, R.A., Lubitz, W., Okamura, M.Y., Paddock, M.L.: EPR study of the molecular and electronic structure of the semiquinone biradical. QA−. QB−. in photosynthetic reaction centers from Rhodobacter sphaeroides. J. Am. Chem. Soc. 122, 7327–7341 (2000)

    Article  Google Scholar 

  • Chesnut, D.B.: On the use of AW2 method for integrated line intensities from first-derivative presentations. J. Magn. Reson. 25, 373–374 (1977)

    Google Scholar 

  • Czoch, R.: Quantitative EPR – sensitivity to experimental conditions and optimal setting of recording parameters. Appl. Magn. Reson. 10, 293–317 (1996)

    Article  Google Scholar 

  • Eastman, M.P., Kooser, R.G., Das, M.R., Freed, J.H.: Heisenberg spin exchange in E.S.R. spectra. I. Linewidth and saturation effects. J. Chem. Phys. 51, 2690–2709 (1969)

    Article  ADS  Google Scholar 

  • Eaton, S.S., Eaton, G.R.: Irradiated fused-quartz standard sample for time-domain EPR. J. Magn. Reson. 102(3), 354–356 (1993)

    Article  MathSciNet  Google Scholar 

  • Eaton, S.S., Eaton, G.R.: Electron paramagnetic resonance. In: Cazes, J. (ed.) Analytical Instrumentation Handbook, 3rd edn, pp. 349–398. Marcel Dekker, New York (2005)

    Google Scholar 

  • Halpern, H.J., Peric, M., Yu, C., Bales, B.L.: Rapid quantitation of parameters from inhomogeneously broadened EPR spectra. J. Magn. Reson. A 103(1), 13–22 (1993)

    Article  Google Scholar 

  • Halpern, H.J., Yu, C., Peric, M., Barth, E., Grdina, D.J., Teicher, B.A.: Oxymetry deep in tissues with low-frequency electron paramagnetic resonance. Proc. Natl. Acad. Sci. U.S.A. 91(26), 13047–13051 (1994)

    Article  ADS  Google Scholar 

  • Hyde, J.S.: Saturation-transfer spectroscopy. Meth. Enzymol. 49, 480–511 (1978)

    Article  Google Scholar 

  • Hyde, J.S., Mett, R.R.: Aqueous sample considerations in uniform field resonators for electron paramagnetic resonance spectroscopy. Curr. Top. Biophys. 26, 7–14 (2002)

    Google Scholar 

  • Hyde, J.S., Mett, R.R., Anderson, J.R.: Cavities with axially uniform fields for use in electron paramag-netic resonance. III. Re-entrant geometries. Rev. Sci. Instrum. 73(11), 4003–4009 (2002)

    Article  ADS  Google Scholar 

  • Hyde, J.S., Thomas, D.D.: Saturation-transfer spectroscopy. Annu. Rev. Phys. Chem. 31, 293–317 (1980)

    Article  ADS  Google Scholar 

  • Joshi, J.P., Eaton, G.R., Eaton, S.S.: Impact of resonator on direct-detected rapid-scan EPR at 9.8 GHz. Appl. Magn. Reson. 29, 239–249 (2005b)

    Article  Google Scholar 

  • Ludowise, P., Eaton, S.S., Eaton, G.R.: A convenient monitor of EPR automatic frequency control function. J. Magn. Reson. 93(2), 410–412 (1991)

    Google Scholar 

  • Mailer, C., Robinson, B.H., Williams, B.B., Halpern, H.J.: Spectral fitting: the extraction of crucial information from a spectrum and a spectral image. Magn. Reson. Med. 49, 1175–1180 (2003)

    Article  Google Scholar 

  • Mailer, C., Sarna, T., Swartz, H.M., Hyde, J.S.: Quantitative studies of free radicals in biology: corrections to ESR saturation data. J. Magn. Reson. 25, 205–210 (1977)

    Google Scholar 

  • Mett, R.R., Froncisz, W., Hyde, J.S.: Axially uniform resonant cavity modes for potential use in electron paramagnetic resonance spectroscopy. Rev. Sci. Instrum. 72(11), 4188–4200 (2001)

    Article  ADS  Google Scholar 

  • Mett, R.R., Hyde, J.S.: Aqueous flat cells perpendicular to the electric field for use in electron paramagnetic resonance spectroscopy. J. Magn. Reson. 165, 137–152 (2003)

    Article  ADS  Google Scholar 

  • More, K.M., Eaton, G.R., Eaton, S.S.: Determination of T1 and T2 by simulation of EPR power saturation curves and saturated spectra. Application to spin-labeled iron porphyrins. J. Magn. Reson. 60(1), 54–65 (1984)

    Google Scholar 

  • Poole, C.P.: Electron Spin Resonance: A Comprehensive Treatise on Experimental Techniques. Interscience, New York (1967)

    Google Scholar 

  • Poole, C.P., Farach, H.: Relaxation in Magnetic Resonance. Academic, New York (1971)

    Google Scholar 

  • Robinson, B.H., Mailer, C., Reese, A.W.: Linewidth analysis of spin labels in liquids. I. Theory and data analysis. J. Magn. Reson. 138, 199–209 (1999a)

    Article  ADS  Google Scholar 

  • Robinson, B.H., Mailer, C., Reese, A.W.: Linewidth analysis of spin labels in liquids. II. Experimental. J. Magn. Reson. 138, 210–219 (1999b)

    Article  ADS  Google Scholar 

  • Schreurs, J.W.H., Fraenkel, G.K.: Anomalous relaxation of hyperfine components in electron spin resonance. II. J. Chem. Phys. 34, 756–758 (1961)

    Article  ADS  Google Scholar 

  • Sidabras, J.W., Mett, R.R., Hyde, J.S.: Aqueous flat cells perpendicular to the electric field for use in electron paramagnetic resonance spectroscopy, II: design. J. Magn. Reson. 172, 333–341 (2005)

    Article  ADS  Google Scholar 

  • Smith, G.W.: Modulation effects in magnetic resonance: widths and amplitudes for Lorentzian and Gaussian lines. J. Appl. Phys. 35, 1217–1221 (1964)

    Article  ADS  Google Scholar 

  • Stoner, J.W., Szymanski, D., Eaton, S.S., Quine, R.W., Rinard, G.A., Eaton, G.R.: Direct-detected rapid-scan EPR at 250 MHz. J. Magn. Reson. 170, 127–135 (2004)

    Article  ADS  Google Scholar 

  • Tseitlin, M., Dhami, A., Quine, R.W., Rinard, G.A., Eaton, S.S., Eaton, G.R.: Electron spin T2 of a nitroxyl radical at 250 MHz measured by rapid scan EPR. Appl. Magn. Reson. 30, 651–656 (2006)

    Article  Google Scholar 

  • Wahlquist, H.: Modulation broadening of unsaturated Lorentzian lines. J. Chem. Phys. 35, 1708–1710 (1961)

    Article  ADS  Google Scholar 

  • Watanabe, T., Sasaki, T., Fujiwara, S.: Phase dependence of saturation transfer EPR signals and estimated rotational correlation times. Appl. Spectrosc. 36, 174 (1982)

    Article  ADS  Google Scholar 

  • Weger, M.: Passage effects in paramagnetic resonance experiments. Bell Syst. Tech. J. 39, 1013–1112 (1960)

    Google Scholar 

  • Weil, J.A., Bolton, J.R., Wertz, J.E.: Electron Paramagnetic Resonance: Elementary Theory and Practical Applications. Wiley, New York (1994b)

    Google Scholar 

  • Wilson, G.V.H.: Modulation broadening of nuclear magnetic resonance and electron spin resonance line shapes. J. Appl. Phys. 34, 3276–3285 (1963)

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Springer-Verlag/Wien

About this chapter

Cite this chapter

Eaton, G.R., Eaton, S.S., Barr, D.P., Weber, R.T. (2010). A More in Depth Look at the EPR Signal Response. In: Quantitative EPR. Springer, Vienna. https://doi.org/10.1007/978-3-211-92948-3_4

Download citation

Publish with us

Policies and ethics