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Advances in Mass Spectrometry-Metabolomics Based Approaches

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Microbial Natural Products Chemistry

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 1439))

Abstract

Highly selective and sensitive analytical techniques are necessary for microbial metabolomics due to the complexity of the microbial sample matrix. Hence, mass spectrometry (MS) has been successfully applied in microbial metabolomics due to its high precision, versatility, sensitivity, and wide dynamic range. The different analytical tools using MS have been employed in microbial metabolomics investigations and can contribute to the discovery or accelerate the search for bioactive substances. The coupling with chromatographic and electrophoretic separation techniques has resulted in more efficient technologies for the analysis of microbial compounds occurring in trace levels. This book chapter describes the current advances in the application of mass spectrometry-based metabolomics in the search for new biologically active agents from microbial sources; the development of new approaches for in silico annotation of natural products; the different technologies employing mass spectrometry imaging to deliver more comprehensive analysis and elucidate the metabolome involved in ecological interactions as they enable visualization of the spatial dispersion of small molecules. We also describe other ambient ionization techniques applied to the fingerprint of microbial natural products and modern techniques such as ion mobility mass spectrometry used to microbial metabolomic analyses and the dereplication of natural microbial products through MS.

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Acknowledgments

The authors aknowledge Coordenação de Aperfeiçoamento de Nível Superior - CAPES, Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq for fellowships and financial support.

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None to declare.

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Authors and Affiliations

  1. Federal University of Goias, Goiânia, GO, Brazil

    Nerilson Marques Lima, Gabriel Franco dos Santos, Gesiane da Silva Lima & Boniek Gontijo Vaz

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  1. Nerilson Marques Lima
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  2. Gabriel Franco dos Santos
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  3. Gesiane da Silva Lima
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  4. Boniek Gontijo Vaz
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Corresponding author

Correspondence to Boniek Gontijo Vaz .

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  1. Institute of Chemistry, Universidade Estadual de Campinas, Campinas, Brazil

    Taícia Pacheco Fill

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Lima, N.M., dos Santos, G.F., da Silva Lima, G., Vaz, B.G. (2023). Advances in Mass Spectrometry-Metabolomics Based Approaches. In: Pacheco Fill, T. (eds) Microbial Natural Products Chemistry. Advances in Experimental Medicine and Biology(), vol 1439. Springer, Cham. https://doi.org/10.1007/978-3-031-41741-2_5

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