Abstract
Real-time detection of airborne infection agents present in human breath and environmental airways, such as the human respiratory Coronavirus, is important for public health. For this, a model label-free immunosensor, based on multi-walled nanotubes (MWNT)-based screen-printed graphite electrodes (SPEs), was proposed and studied. For sensing applications, MWNTs have many advantages such as small size with larger surface area, excellent electron transfer promoting ability when used for antibody immobilization, with retention of its selectivity for potential immunosensors development. In order to verify the selectivity of the selected primary antibody (anti-CoV 229E antibody) and the effective immunocomplex formation (antigen-antibody), an in-depth voltammetric characterization of MWNT-SPEs interface was carried out during the multistep fabrication of CoV immunosensor using [Fe(CN)6]3−/4− as an electroactive probe. After that, the analytical robustness of the performances of these immunosensing platforms was estimated and verified. Indeed, a nanomolar range detection limit (180 TCID50/mL)g/mL) with excellent reproducibility (RSD% = 8%) was obtained.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Fung, F., Wang, H.-S., Menon, S.: Food safety in the 21st century. Biomed. J. 41, 88–95 (2018). https://doi.org/10.1016/j.bj.2018.03.003
Patel, M., Kumar, R., Kishor, K., Mlsna, T., Pittman, C.U., Mohan, D.: Pharmaceuticals of emerging concern in aquatic systems: chemistry, occurrence, effects, and removal methods. Chem. Rev. 119, 3510–3673 (2019). https://doi.org/10.1021/acs.chemrev.8b00299
Mitchell, M.J., Billingsley, M.M., Haley, R.M., Wechsler, M.E., Peppas, N.A., Langer, R.: Engineering precision nanoparticles for drug delivery. Nat. Rev. Drug Discov. 20, 101–124 (2021). https://doi.org/10.1038/s41573-020-0090-8
Alahi, M., Mukhopadhyay, S.: Detection methodologies for pathogen and toxins: a review. Sensors 17, 1885 (2017). https://doi.org/10.3390/s17081885
Cancelliere, R., et al.: Electrochemical and morphological layer-by-layer characterization of electrode interfaces during a label-free impedimetric immunosensor build-up: the case of ochratoxin A. Appl. Surf. Sci. 567, 150791 (2021). https://doi.org/10.1016/j.apsusc.2021.150791
Zhang, Y., et al.: Label-free electrochemical immunosensor based on enhanced signal amplification between Au@Pd and CoFe2O4/graphene nanohybrid. Sci. Rep. 6, 23391 (2016). https://doi.org/10.1038/srep23391
Coronaviridae. In: Fenner’s Veterinary Virology, pp. 393–413. Elsevier (2011). https://doi.org/10.1016/B978-0-12-375158-4.00024-9
Cancelliere, R., Tinno, A.D., Cataldo, A., Bellucci, S., Micheli, L.: Powerful electron-transfer screen-printed platforms as biosensing tools: the case of uric acid biosensor. Biosensors 12, 2 (2021). https://doi.org/10.3390/bios12010002
Cancelliere, R., Di Tinno, A., Di Lellis, A.M., Contini, G., Micheli, L., Signori, E.: Cost-effective and disposable label-free voltammetric immunosensor for sensitive detection of interleukin-6. Biosens. Bioelectron. 114467 (2022). https://doi.org/10.1016/j.bios.2022.114467
Funding
This work was partially supported by the Project “SFIDE A SMART FRAMEWORK FOR VIRUS DETECTION”, funded by Italian Ministry of University and Research FISR DD 562/2020 - FONDO INTEGRATIVO SPECIALE PER LA RICERCA, grant # FISR2020IP_02585.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this paper
Cite this paper
Cancelliere, R. et al. (2023). Coronavirus Label-Free Immunosensor: Preliminary Results. In: Di Francia, G., Di Natale, C. (eds) Sensors and Microsystems. AISEM 2022. Lecture Notes in Electrical Engineering, vol 999. Springer, Cham. https://doi.org/10.1007/978-3-031-25706-3_3
Download citation
DOI: https://doi.org/10.1007/978-3-031-25706-3_3
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-031-25705-6
Online ISBN: 978-3-031-25706-3
eBook Packages: EngineeringEngineering (R0)