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Microbiome in 3P Medicine Strategies pp 371–390Cite as

Probiotic Concepts of Predictive, Preventive, and Personalized Medical Approach for Obesity: Lactic Acid Bacteria and Bifidobacteria Probiotic Strains Improve Glycemic and Inflammation Profiles

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Part of the book series: Advances in Predictive, Preventive and Personalised Medicine ((APPPM,volume 16))

Abstract

The use of probiotics demonstrate efficacy against obesity and metabolic syndrome (MetS). Detection of effective probiotic strains for hyperglycemia and immunity correction is important task. Current study was evaluating an influence of Lactobacillus casei IMV B-7280 separately and composition L. casei IMV B-7280 / Bifidobacterium animalis VKB / B. animalis VKL on the levels of blood glucose and immunity in obese mice. Obesity was induced by fat-enriched diet (FED) in male BALB/c mice. Obese mice were transferred to standard diet and received per os probiotic strains daily during 10 days. We measured tumor necrosis factor-alpha (TNF-alpha) in blood serum using enzyme-linked immunosorbent assay and functional activity of peritoneal exudate macrophages (PEMs). Glucose levels in blood were defined with glucometer.

We ascertained that all probiotic strains induced reducing mice weight and visceral fat, normalization of TNF-alpha production and functional activity of PEMs. Treatment with L. casei IMV B-7280 was associated with decreasing blood glucose levels. No normalization of glucose and TNF-alpha levels was observed in obese mice, transferred to standard diet without probiotic treatment; although we revealed decreasing their weight and visceral fat and partial recover of functional activity of PEMs. In conclusion, probiotic strain L. casei IMV B-7280 (separately) and composition L. casei IMV B-7280 / B. animalis VKB / B. animalis VKL can re-equilibrate metabolic and inflammation indices in mouse obesity model. L. casei IMV B-7280 alone was more efficient in decreasing glucose levels than composition of strains.

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Abbreviations

FED:

Fat-enriched diet

LAB:

Lactic acid bacteria

FR:

Functional reserve

HDL :

High density lipoprotein

IL:

Interleukin

LPS :

Lipopolysaccharides

TNF-α:

Tumor necrosis factor-α

IFN- γ:

Interferon-γ

MetS:

Metabolic syndrome

NBT:

Nitro-blue tetrazolium

PEMs:

Peritoneal exudate macrophages

PN:

Phagocytic number

PBS:

Phosphate buffered saline

PI:

Phagocytic index

RBA:

Respiratory burst activity

SCFA :

Short-chain fatty acid

DM :

Diabetes mellitus

T2DM :

Type 2 diabetes mellitus

FBG:

Fasting blood glucose

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Ethics

This study has been approved by the ethics committee of institutional review board and Special Academic Council on Doctoral Thesis of D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine (protocol N 7 issued 03.07.2018). No human subjects were included to the study.

Funding

This research received financial support from the State Fund for Fundamental Research of Ukraine via the framework of the project Ф64/28–2015.

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Authors and Affiliations

  1. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    Liudmyla Lazarenko, Oleksandra Melnykova, Lidiia Babenko, Rostyslav Bubnov & Mykola Spivak

  2. ESC “Institute of Biology and Medicine” of Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

    Oleksandra Melnykova, Tetyana Beregova & Tetyana Falalyeyeva

  3. PJSC «SPC Diaproph-Med», Kyiv, Ukraine

    Mykola Spivak

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  1. Liudmyla Lazarenko
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  2. Oleksandra Melnykova
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  4. Rostyslav Bubnov
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  5. Tetyana Beregova
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Editors and Affiliations

  1. Clinical Laboratory Diagnostics and Pharmacology, Uzhhorod National University & Ediens LLC, Uzhhorod, Ukraine

    Nadiya Boyko

  2. 3P Medicine, Radiation Oncology, Universitätsklinik Bonn, Bonn, Nordrhein-Westfalen, Germany

    Olga Golubnitschaja

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Lazarenko, L. et al. (2023). Probiotic Concepts of Predictive, Preventive, and Personalized Medical Approach for Obesity: Lactic Acid Bacteria and Bifidobacteria Probiotic Strains Improve Glycemic and Inflammation Profiles. In: Boyko, N., Golubnitschaja, O. (eds) Microbiome in 3P Medicine Strategies. Advances in Predictive, Preventive and Personalised Medicine, vol 16. Springer, Cham. https://doi.org/10.1007/978-3-031-19564-8_14

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