Abstract
The use of probiotics demonstrate efficacy against obesity and metabolic syndrome (MetS). Detection of effective probiotic strains for hyperglycemia and immunity correction is important task. Current study was evaluating an influence of Lactobacillus casei IMV B-7280 separately and composition L. casei IMV B-7280 / Bifidobacterium animalis VKB / B. animalis VKL on the levels of blood glucose and immunity in obese mice. Obesity was induced by fat-enriched diet (FED) in male BALB/c mice. Obese mice were transferred to standard diet and received per os probiotic strains daily during 10 days. We measured tumor necrosis factor-alpha (TNF-alpha) in blood serum using enzyme-linked immunosorbent assay and functional activity of peritoneal exudate macrophages (PEMs). Glucose levels in blood were defined with glucometer.
We ascertained that all probiotic strains induced reducing mice weight and visceral fat, normalization of TNF-alpha production and functional activity of PEMs. Treatment with L. casei IMV B-7280 was associated with decreasing blood glucose levels. No normalization of glucose and TNF-alpha levels was observed in obese mice, transferred to standard diet without probiotic treatment; although we revealed decreasing their weight and visceral fat and partial recover of functional activity of PEMs. In conclusion, probiotic strain L. casei IMV B-7280 (separately) and composition L. casei IMV B-7280 / B. animalis VKB / B. animalis VKL can re-equilibrate metabolic and inflammation indices in mouse obesity model. L. casei IMV B-7280 alone was more efficient in decreasing glucose levels than composition of strains.
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- FED:
-
Fat-enriched diet
- LAB:
-
Lactic acid bacteria
- FR:
-
Functional reserve
- HDL :
-
High density lipoprotein
- IL:
-
Interleukin
- LPS :
-
Lipopolysaccharides
- TNF-α:
-
Tumor necrosis factor-α
- IFN- γ:
-
Interferon-γ
- MetS:
-
Metabolic syndrome
- NBT:
-
Nitro-blue tetrazolium
- PEMs:
-
Peritoneal exudate macrophages
- PN:
-
Phagocytic number
- PBS:
-
Phosphate buffered saline
- PI:
-
Phagocytic index
- RBA:
-
Respiratory burst activity
- SCFA :
-
Short-chain fatty acid
- DM :
-
Diabetes mellitus
- T2DM :
-
Type 2 diabetes mellitus
- FBG:
-
Fasting blood glucose
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Ethics
This study has been approved by the ethics committee of institutional review board and Special Academic Council on Doctoral Thesis of D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine (protocol N 7 issued 03.07.2018). No human subjects were included to the study.
Funding
This research received financial support from the State Fund for Fundamental Research of Ukraine via the framework of the project Ф64/28–2015.
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Lazarenko, L. et al. (2023). Probiotic Concepts of Predictive, Preventive, and Personalized Medical Approach for Obesity: Lactic Acid Bacteria and Bifidobacteria Probiotic Strains Improve Glycemic and Inflammation Profiles. In: Boyko, N., Golubnitschaja, O. (eds) Microbiome in 3P Medicine Strategies. Advances in Predictive, Preventive and Personalised Medicine, vol 16. Springer, Cham. https://doi.org/10.1007/978-3-031-19564-8_14
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