Abstract
In vivo reflectance confocal microscope (RCM) is a high-resolution noninvasive imaging technique that was initially focused on the diagnosis of skin cancers. A rising number of other indications have been later described among which the diagnosis and management of infectious dermatological disorders. RCM is particularly suitable for the identification of Sarcoptes scabies, Demodex folliculorum, Ixodes, Dermatophytes, and Candida species in the clinical practice and for the follow-up after treatment. RCM cannot observe virus directly. However, the cytopathic effect associated with some virus can be recognized even in a pre-vesicular stage. In addition, thanks to its noninvasiveness, RCM allows pathophysiological studies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Piérard GE. In vivo confocal microscopy: a new paradigm in dermatology. Dermatol Basel Switz. 1993;186(1):4–5.
Corcuff P, Bertrand C, Leveque JL. Morphometry of human epidermis in vivo by real-time confocal microscopy. Arch Dermatol Res. 1993;285(8):475–81.
Goldgeier M, Alessi C, Muhlbauer JE. Immediate noninvasive diagnosis of herpesvirus by confocal scanning laser microscopy. J Am Acad Dermatol. 2002;46(5):783–5.
Abraham LS, Costa MC, Agozzino M, Amorosi B, Cota C, Ardigo M. In vivo reflectance confocal microscopy for varicella prompt diagnosis and treatment in a severely immunosuppressed patient. Skin Res Technol. 2012;18(3):386–8.
Scope A, Benvenuto-Andrade C, Gill M, Ardigo M, Gonzalez S, Marghoob AA. Reflectance confocal microscopy of molluscum contagiosum. Arch Dermatol. 2008;144(1):134.
Leclercq A, et al. Apport de la microscopie confocale par réflectance dans le diagnostic de syphilides ano-vulvaires, premier cas relaté. Ann Dermatol Vénéréologie. 2016;143(11):687–90.
Longo C, Pellacani G, Ricci C, De Pace B, Argenziano G, Zalaudek I. In vivo detection of Demodex folliculorum by means of confocal microscopy. Br J Dermatol. 2012;166(3):690–2.
Slutsky JB, Rabinovitz H, Grichnik JM, Marghoob AA. Reflectance confocal microscopic features of dermatophytes, scabies, and demodex. Arch Dermatol. 2011;147(8):1008.
Longo C, Bassoli S, Monari P, Seidenari S, Pellacani G. Reflectance-mode confocal microscopy for the in vivo detection of Sarcoptes scabiei. Arch Dermatol. 2005;141(10):1336.
Cinotti E, Perrot J-L, Labeille B, Cambazard F. On the feasibility of confocal microscopy for the diagnosis of scabies. Ann Dermatol Venereol. 2013;140(3):215–6.
Perrot J-L, et al. Rapid diagnosis of scabies by manual confocal reflectance microscopy. Ann Dermatol Venereol. 2012;139(6–7):502–5.
Levi A, Mumcuoglu KY, Ingber A, Enk CD. Assessment of Sarcoptes scabiei viability in vivo by reflectance confocal microscopy. Lasers Med Sci. 2011;26(3):291–2.
Turan E, Erdemir AT, Gurel MS, Yurt N. A new diagnostic technique for tinea incognito: in vivo reflectance confocal microscopy. Report of five cases. Skin Res Technol. 2013;19(1):e103–7.
Cinotti E, et al. Reflectance confocal microscopy for quantification of Sarcoptes scabiei in Norwegian scabies. J Eur Acad Dermatol Venereol. 2013;27(2):e176–8.
Sattler EC, Maier T, Hoffmann VS, Hegyi J, Ruzicka T, Berking C. Noninvasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy. Br J Dermatol. 2012;167(5):1042–7.
Veasey J, Framil V, Ribeiro A, Lellis R. Reflectance confocal microscopy use in one case of Pityriasis folliculorum: a Demodex folliculorum analysis and comparison to other diagnostic methods. Int J Dermatol. 2014;53(4):e254–7.
Yuan C, et al. Comparison of reflectance confocal microscopy and standardized skin surface biopsy for three different lesions in a pityriasis folliculorum patient. Br J Dermatol. 2015;172(5):1440–2.
Turgut Erdemir A, et al. Reflectance confocal microscopy vs. standardized skin surface biopsy for measuring the density of Demodex mites. Skin Res Technol Off. 2014;20(4):435–9.
Del Giudice P, et al. Pyemotes ventricosus dermatitis, southeastern France. Emerg Infect Dis. 2008;14(11):1759–61.
Cinotti E, Espinasse M, Labeille B, Cambazard F, Perrot JL. Dermoscopy, confocal microscopy and optical coherence tomography for the diagnosis of bedbug infestation. J Eur Acad Dermatol Venereol. 2017;31(4):e203–4.
Erdoğan S, Dorittke P, Kardorff B. Identification of a foreign body--tick (Ixodes)--with confocal laser scan microscopy (CLSM) in comparison to histology in a mouse model. J Dtsch Dermatol. 2012;10(4):277–8.
Dupuy A, et al. Accuracy of standard dermoscopy for diagnosing scabies. J Am Acad Dermatol. 2007;56(1):53–62.
Levi A, Mumcuoglu KY, Ingber A, Enk CD. Detection of living Sarcoptes scabiei larvae by reflectance mode confocal microscopy in the skin of a patient with crusted scabies. J Biomed Opt. 2012;17(6):060503.
Cinotti E, et al. Videodermoscopy compared to reflectance confocal microscopy for the diagnosis of scabies. J Eur Acad Dermatol Venereol. 2016;30(9):1573–7.
Lacey N, Forton FMN, Powell FC. Demodex quantification methods: limitations of confocal laser scanning microscopy. Br J Dermatol. 2013;169(1):212–3.
Cinotti E, Fiorani D, Labeille B, Cambazard F, Rubegni P, Perrot JL. Demodex induced Blepharitis diagnosed by in vivo confocal microscopy. J Dermatol Pigm Res. 2017;1(1):1–3.
Sattler EC, Maier T, Hoffmann VS, Ruzicka T, Berking C. Noninvasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy: reply from the authors. Br J Dermatol. 2013;169(1):213–5.
Randon M, et al. In vivo confocal microscopy as a novel and reliable tool for the diagnosis of Demodex eyelid infestation. Br J Ophthalmol. 2015;99(3):336–41.
Alarcon I, Carrera C, Puig S, Malvehy J. In vivo confocal microscopy features of cutaneous leishmaniasis. Dermatol Basel Switz. 2014;228(2):121–4.
Buljan M, Zalaudek I, Massone C, Hofmann-Wellenhof R, Fink-Puches R, Arzberger E. Dermoscopy and reflectance confocal microscopy in cutaneous leishmaniasis on the face: Dermoscopy and RCM of skin leishmaniasis. Australas J Dermatol. 2016;57(4):316–8.
Cinotti E, Perrot JL, Labeille B, Cambazard F. Reflectance confocal microscopy for cutaneous infections and infestations. J Eur Acad Dermatol Venereol. 2016;30(5):754–63.
Cinotti E, et al. Tinea corporis diagnosed by reflectance confocal microscopy. Ann Dermatol Venereol. 2014;141(2):150–2.
Pharaon M, et al. Diagnosis and treatment monitoring of toenail onychomycosis by reflectance confocal microscopy: prospective cohort study in 58 patients. J Am Acad Dermatol. 2014;71(1):56–61.
Piérard GE, et al. Microscopic diagnosis of onychomycoses. Ann Dermatol Venereol. 1994;121(1):25–9.
Hongcharu W, Dwyer P, Gonzalez S, Anderson RR. Confirmation of onychomycosis by in vivo confocal microscopy. J Am Acad Dermatol. 2000;42(2 Pt 1):214–6.
Markus R, Huzaira M, Anderson RR, González S. A better potassium hydroxide preparation? In vivo diagnosis of tinea with confocal microscopy. Arch Dermatol. 2001;137(8):1076–8.
Cinotti E, Fouilloux B, Perrot JL, Labeille B, Douchet C, Cambazard F. Confocal microscopy for healthy and pathological nail. J Eur Acad Dermatol Venereol. 2014;28(7):853–8.
Cinotti E, Perrot JL, Labeille B, Raberin H, Flori P, Cambazard F. Hair dermatophytosis diagnosed by reflectance confocal microscopy: six cases. J Eur Acad Dermatol Venereol. 2015;29(11):2257–9.
Rothmund G, et al. Confocal laser scanning microscopy as a new valuable tool in the diagnosis of onychomycosis—comparison of six diagnostic methods. Mycoses. 2013;56(1):47–55.
Liansheng Z, Xin J, Cheng Q, Zhiping W, Yanqun L. Diagnostic applicability of confocal laser scanning microscopy in tinea corporis. Int J Dermatol. 2013;52(10):1281–2.
Hui D, Xue-cheng S, Ai-e X. Evaluation of reflectance confocal microscopy in dermatophytosis. Mycoses. 2013;56(2):130–3.
Arrese J-E, Quatresooz P, Piérard-Franchimont C, Piérard G-E. Nail histomycology. Protean aspects of a human fungal bed. Ann Dermatol Venereol. 2003;130(12 Pt 2):1254–9.
Venturini M, Sala R, Semenza D, Santoro A, Facchetti F, Calzavara-Pinton P. Reflectance confocal microscopy for the in vivo detection of Treponema pallidum in skin lesions of secondary syphilis. J Am Acad Dermatol. 2009;60(4):639–42.
Tognetti L, et al. Ecthyma contagiosum (Orf): Reflectance confocal microscopy and histopathological correlates. Skin Res. Technol. 2019;25(2):234–37.
Almodovar JL, Schifitto G, McDermott MP, Ferguson M, Herrmann DN. HIV neuropathy: an in vivo confocal microscopic study. J Neurovirol. 2012;18(6):503–10.
Cinotti E, et al. How transparent film applied on dermatologic imaging devices in order to prevent infections affects image quality? Skin Res. Technol. 2019;25(2):229–33.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Provvidenziale, L., Perrot, J.L., Cinotti, E. (2020). In Vivo Reflectance Confocal Microscopy for Infectious Diseases. In: Fimiani, M., Rubegni, P., Cinotti, E. (eds) Technology in Practical Dermatology. Springer, Cham. https://doi.org/10.1007/978-3-030-45351-0_18
Download citation
DOI: https://doi.org/10.1007/978-3-030-45351-0_18
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-45350-3
Online ISBN: 978-3-030-45351-0
eBook Packages: MedicineMedicine (R0)