Abstract
Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.
Authors Hannah Degeling and Sarah S. Bovenberg have contributed equally to this chapter.
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Acknowledgements
This work was supported by grant from NIH/NINDS P30NS045776 and 1R01NS064983 (BAT). M. Hannah Degeling is supported by a Fulbright scholarship, the Saal van Zwanenberg Foundation, VSB fonds, Dr. Hendrik Muller Vaderlandschfonds, the Dutch Cancer Foundation (KWF Kankerbestrijding), the Hersenstichting brain fund as well as the Jo Keur (Leiden hospital). M. Sarah Bovenberg was supported by a Fulbright scholarship, the Huygens Scholarship Program, VSB fonds and the Saal van Zwanenberg Foundation. We thank Kristan van der Vos for assistance with cell culture.
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Degeling, M.H., Bovenberg, M.S.S., Tannous, M., Tannous, B.A. (2014). Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture. In: Badr, C. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 1098. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-718-1_4
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DOI: https://doi.org/10.1007/978-1-62703-718-1_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-717-4
Online ISBN: 978-1-62703-718-1
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