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HIV-1 Reverse Transcription

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Human Retroviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1087))

Abstract

Reverse transcription is an obligatory step in retrovirus replication in the course of which the retroviral RNA/DNA-dependent DNA polymerase (RT) copies the single-stranded positive sense RNA genome to synthesize the double-stranded viral DNA. At the same time the RT-associated RNaseH activity degrades the genomic RNA template, which has just been copied. The viral nucleocapsid protein NCp7 is an obligatory partner of RT, chaperoning synthesis of the complete viral DNA flanked by the two long-terminal repeats (LTR), required for viral DNA integration into the host genome and its expression. Here we describe assays for in vitro and ex vivo monitoring of reverse transcription and the chaperoning role of the nucleocapsid protein (NC).

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Acknowledgments

Work in the laboratory is supported by grants from INSERM, CNRS, ANRS, EC (6th PCRDT), FINOVI, and Sidaction (France).

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Authors and Affiliations

  1. Centre International de Recherche en Infectiologie (CIRI)- INSERM U1111 – CNRS UMR5308- Université Lyon 1- ENS de Lyon, Lyon, France

    Andrea Cimarelli

  2. Départment de Virologie Humaine, Faculté de Pharmacie, ILLKIRCH, France

    Jean-Luc Darlix

Authors
  1. Andrea Cimarelli
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  2. Jean-Luc Darlix
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Editors and Affiliations

  1. Div. Immunology, Transplantation, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy

    Elisa Vicenzi

  2. Div. Immunology, Transplantation, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy

    Guido Poli

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Cimarelli, A., Darlix, JL. (2014). HIV-1 Reverse Transcription. In: Vicenzi, E., Poli, G. (eds) Human Retroviruses. Methods in Molecular Biology, vol 1087. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-670-2_6

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  • DOI: https://doi.org/10.1007/978-1-62703-670-2_6

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-669-6

  • Online ISBN: 978-1-62703-670-2

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