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High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine

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Virus-Host Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1064))

Abstract

Gene expression profiling of changes in total RNA levels has provided invaluable knowledge on the regulation of gene expression. Studies on the kinetics of this regulation, however, have been limited by the fact that total cellular RNA is a poor template for revealing short-term changes in gene expression, alterations in RNA decay rates and the kinetics of RNA processing as well as the differentiation thereof. Here, we describe the metabolic labeling and purification of newly transcribed RNA with 4-thiouridine to study the molecular mechanisms governing RNA synthesis, processing, and decay.

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Acknowledgments

This work was supported by NGFN Plus grant #01GS0801, MRC fellowship grant G1002523, and NHSBT grant WP11-05 to L.D.

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Authors and Affiliations

  1. Department of Medicine, Addenbrooke’s Hospital, University of Cambridge, Cambridge, UK

    Lars Dölken

Authors
  1. Lars Dölken
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Editor information

Editors and Affiliations

  1. University of Stuttgart Institute of Interfacial Process, Stuttgart, Germany

    Susanne M. Bailer

  2. Ulm University Medical Center Institute of Virology, Ulm, Germany

    Diana Lieber

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Dölken, L. (2013). High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine. In: Bailer, S., Lieber, D. (eds) Virus-Host Interactions. Methods in Molecular Biology, vol 1064. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-601-6_6

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  • DOI: https://doi.org/10.1007/978-1-62703-601-6_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-600-9

  • Online ISBN: 978-1-62703-601-6

  • eBook Packages: Springer Protocols

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