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Methods of Cellular Senescence Induction Using Oxidative Stress

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Book cover Biological Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1048))

Abstract

Normal somatic cells do not divide indefinitely and have their finite replicative lifespan. This property leads to an eventual arrest of cell division termed cell senescence. Human diploid fibroblasts offer a typical model for studying cell senescence in vitro. Various approaches to evoke oxidative stresses, such as the exposures of cells to ultraviolet light, ethanol, tert-butyl hydroperoxide (t-BHP), and peroxide hydrogen (H2O2), have been used to study the onset of cellular senescence. The early onset of cellular senescence induced by these stresses is termed stress-induced premature senescence (SIPS). In this manuscript, we will mainly summarize the basic knowledge and experimental approaches important for the induction of SIPS by H2O2, since H2O2 is the most commonly used inducer of SIPS in vitro and an endogenous source of cellular oxidative stress. Several assays methods generally used for testifying cell senescence are introduced.

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Acknowledgment

This work was supported by the grant from National Natural Science Foundation of China (Grant Number 81273224), and from West China Hospital of Sichuan University for (Huaxi Grant 137080022). We thank all team members in our lab for the wonderful cooperation.

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Authors and Affiliations

  1. Department of Geriatrics, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, People’s Republic of China

    Zhe Wang, Dandan Wei & Hengyi Xiao

Authors
  1. Zhe Wang
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  2. Dandan Wei
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  3. Hengyi Xiao
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Editors and Affiliations

  1. Dept. Biology, University of Alabama, Birmingham Dept. Biology, Birmingham, Alabama, USA

    Trygve O. Tollefsbol

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Wang, Z., Wei, D., Xiao, H. (2013). Methods of Cellular Senescence Induction Using Oxidative Stress. In: Tollefsbol, T. (eds) Biological Aging. Methods in Molecular Biology, vol 1048. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-556-9_11

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  • DOI: https://doi.org/10.1007/978-1-62703-556-9_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-555-2

  • Online ISBN: 978-1-62703-556-9

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