Abstract
Loop-mediated isothermal amplification (LAMP), which employs a Bst DNA polymerase with strand-displacement activity and four primers (two inner primers and two outer primers) recognizing six distinct regions on the target DNA, is a highly sensitive, specific, simple, and rapid nucleotide amplification method. Moreover, because the Bst DNA polymerase resists much DNA polymerase inhibitors present in biological specimens, the LAMP method is suitable for the detection of infectious agents from clinical material such as fecal samples. Here, we describe the LAMP method which can differentially detect and identify human Taenia tapeworms, Taenia solium, T. saginata, and T. asiatica, using DNA specimens prepared from parasite tissue and human fecal sample.
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References
Ito A, Nakao M, Wandra T (2003) Human taeniasis and cysticercosis in Asia. Lancet 362:1918–1920
Garcia HH, Gonzalez AE, Evans CA, Gilman RH (2003) Taenia solium cysticercosis. Lancet 362:547–556
Schantz PM, Cruz M, Sarti E, Pawlowski Z (1993) Potential eradicability of Taeniasis and cysticercosis. Bull Pan Am Health Organ 27:397–403
Gonzalez LM, Montero E, Harrison LJ, Parkhouse RM, Garate T (2000) Differential diagnosis of Taenia saginata and Taenia solium infection by PCR. J Clin Microbiol 38:737–744
Yamasaki H, Allan JC, Sato MO, Nakao M, Sako Y, Nakaya K et al (2004) DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR. J Clin Microbiol 42:548–553
Nunes CM, Dias AK, Dias FE, Aoki SM, de Paula HB, Lima LG et al (2005) Taenia saginata: differential diagnosis of human taeniasis by polymerase chain reaction-restriction fragment length polymorphism assay. Exp Parasitol 110:412–415
Mayta H, Gilman RH, Prendergast E, Castillo JP, Tinoco YO, Garcia HH et al (2008) Nested PCR for specific diagnosis of Taenia solium taeniasis. J Clin Microbiol 46:286–289
Abu AlSoud W, Radstrom P (2000) Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J Clin Microbiol 38:4463–4470
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N et al (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63
Mori Y, Nagamine K, Tomita N, Notomi T (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150–154
Bakheit MA, Torra D, Palomino LA, Thekisoe OM, Mbati PA, Ongerth J et al (2008) Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing. Vet Parasitol 158:11–22
Liang SY, Chan YH, Hsia KT, Lee JL, Kuo MC, Hwa KY et al (2009) Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica. J Clin Microbiol 47:1892–1895
Macuhova K, Kumagai T, Akao N, Ohta N (2010) Loop-mediated isothermal amplification assay for detection and discrimination of Toxocara canis and Toxocara cati eggs directly from sand samples. J Parasitol 96:1224–1227
Mori Y, Notomi T (2009) Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother 15:62–69
Nkouawa A, Sako Y, Nakao M, Nakaya K, Ito A (2009) Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species. J Clin Microbiol 47:168–174
Nkouawa A, Sako Y, Li T, Chen X, Wandra T, Swastika IK et al (2010) Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans. J Clin Microbiol 48:3350–3352
Nunes CM, Lima LG, Manoel CS, Pereira RN, Nakano MM, Garcia JF (2006) Fecal specimens preparation methods for PCR diagnosis of human taeniosis. Rev Inst Med Trop Sao Paulo 48:45–47
Nkouawa A, Sako Y, Li T, Chen X, Nakao M, Yanagida T et al (2012) A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey. Parasitol Int 61:723–725
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Sako, Y., Nkouawa, A., Yanagida, T., Ito, A. (2013). Loop-Mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms. In: Kolpashchikov, D., Gerasimova, Y. (eds) Nucleic Acid Detection. Methods in Molecular Biology, vol 1039. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-535-4_9
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DOI: https://doi.org/10.1007/978-1-62703-535-4_9
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Publisher Name: Humana Press, Totowa, NJ
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