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Genotoxicity Assessment

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Alok Dhawan

    1. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

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    You can also search for this editor in PubMed   Google Scholar

  2. Mahima Bajpayee

    1. School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Describes comprehensive genetic toxicology protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1044)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
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  2. Gene Mutation Assays

    1. Front Matter

      Pages 1-1
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    2. Bacterial Mutation Assays

      • Errol Zeiger
      Pages 3-26

    3. In Vitro Mouse Lymphoma (L5178Y Tk +/− -3.7.2C) Forward Mutation Assay

      • Melissa R. Schisler, Martha M. Moore, B. Bhaskar Gollapudi
      Pages 27-50

    4. Erythrocyte-Based Pig-a Gene Mutation Assay

      • Jeffrey C. Bemis, Nikki E. Hall, Stephen D. Dertinger
      Pages 51-77

    5. Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

      • Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, Robert H. Heflich
      Pages 79-95

    6. In Vivo cII, gpt, and Spi− Gene Mutation Assays in Transgenic Mice and Rats

      • Mugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, Robert H. Heflich
      Pages 97-119

  3. Assays for Chromosomal Abnormalities

    1. Front Matter

      Pages 121-121
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    2. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

      • Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, Fabrizio Palitti
      Pages 123-146

    3. Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse

      • Francesca Pacchierotti, Valentina Stocchi
      Pages 147-163

    4. Chromosomal Aberration Test in Human Lymphocytes

      • Christian Johannes, Guenter Obe
      Pages 165-178

    5. In Vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood

      • Sawako Kasamoto, Shoji Masumori, Makoto Hayashi
      Pages 179-189

    6. Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells

      • Claudia Bolognesi, Michael Fenech
      Pages 191-207

    7. Flow Cytometric Determination of Micronucleus Frequency

      • Azeddine Elhajouji, Magdalena Lukamowicz-Rajska
      Pages 209-235

    8. Fluorescence In Situ Hybridization (FISH) Technique for the Micronucleus Test

      • Ilse Decordier, Micheline Kirsch-Volders
      Pages 237-244

    9. Comparative Genomic Hybridization (CGH) in Genotoxicology

      • Adolf Baumgartner
      Pages 245-268

    10. The In Vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity

      • Bella B. Manshian, Neenu Singh, Shareen H. Doak
      Pages 269-289

  4. Tests for Primary DNA Damage

    1. Front Matter

      Pages 291-291
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    2. γ-H2AX Detection in Somatic and Germ Cells of Mice

      • Eugenia Cordelli, Lorena Paris
      Pages 293-310

    3. Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers

      • Julian Laubenthal, Michal R. Gdula, Alok Dhawan, Diana Anderson
      Pages 311-323
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Keywords

About this book

Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.

Editors and Affiliations

  • School of Science and Technology, Ahmedabad University Institute of Life Sciences, Ahmedabad, India

    Alok Dhawan, Mahima Bajpayee

Bibliographic Information

  • Book Title: Genotoxicity Assessment

  • Book Subtitle: Methods and Protocols

  • Editors: Alok Dhawan, Mahima Bajpayee

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-529-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-528-6Published: 30 July 2013

  • Softcover ISBN: 978-1-4939-6302-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-529-3Published: 30 July 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 463

  • Number of Illustrations: 44 b/w illustrations, 62 illustrations in colour

  • Topics: Pharmacology/Toxicology, Human Genetics

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