Abstract
Mouse models have proven to be a key approach in our understanding of the etiology and physiology underlying bacterial mediated gastrointestinal inflammation. Generally, these models are based on the inoculation of genetically susceptible mice with either commensal or pathogenic bacteria to elicit an inflammatory response. Here, we describe models of acute and chronic gastrointestinal inflammation using interleukin 10-deficient (Il10 −/−) mice colonized with the pathogenic Campylobacter jejuni strain 81-176 or the commensal Escherichia coli strain NC 101.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Frank DN, St Amand AL, Feldman RA, Boedeker EC, Harpaz N, Pace NR (2007) Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proc Natl Acad Sci U S A 104(34):13780–13785
Neish AS (2009) Microbes in gastrointestinal health and disease. Gastroenterology 136(1):65–80
Turnbaugh PJ, Ley RE, Hamady M, Fraser-Liggett CM, Knight R, Gordon JI (2007) The human microbiome project. Nature 449(7164):804–810
Haller D, Jobin C (2004) Interaction between resident luminal bacteria and the host: can a healthy relationship turn sour? J Pediatr Gastroenterol Nutr 38(2):123–136
Mantovani A (2005) Cancer: inflammation by remote control. Nature 435(7043):752–753
Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jones JL, Griffin PM (2011) Foodborne illness acquired in the United States–major pathogens. Emerg Infect Dis 17(1):7–15
Blaser MJ (1997) Epidemiologic and clinical features of Campylobacter jejuni infections. J Infect Dis 176(Suppl 2):S103–S105
Sellon RK, Tonkonogy S, Schultz M, Dieleman LA, Grenther W, Balish E, Rennick DM, Sartor RB (1998) Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. Infect Immun 66(11):5224–5231
Lippert E, Karrasch T, Sun X, Allard B, Herfarth HH, Threadgill D, Jobin C (2009) Gnotobiotic IL-10; NF-kappaB mice develop rapid and severe colitis following Campylobacter jejuni infection. PLoS One 4(10):e7413
Sun X, Threadgill D, Jobin C (2012) Campylobacter jejuni induces colitis through activation of mammalian target of rapamycin signaling. Gastroenterology 142(1):86–95.e85
Patwa LG, Fan TJ, Tchaptchet S, Liu Y, Lussier YA, Sartor RB, Hansen JJ (2011) Chronic intestinal inflammation induces stress-response genes in commensal Escherichia coli. Gastroenterology 141(5):1842–1851, e1841–1810
Kim SC, Tonkonogy SL, Karrasch T, Jobin C, Sartor RB (2007) Dual-association of gnotobiotic IL-10−/− mice with 2 nonpathogenic commensal bacteria induces aggressive pancolitis. Inflamm Bowel Dis 13(12):1457–1466
Acknowledgments
We would like to acknowledge Ryan Balfour Sartor M.D. and Dr. Christian Jobin Ph.D. in the Center for Gastrointestinal Biology and Disease at the University of North Carolina at Chapel Hill for establishing these protocols through their research efforts in the investigation of bacterial induced intestinal epithelial inflammation.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Uronis, J., Sun, X. (2013). Bacterial Mediated Gastrointestinal Inflammation. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1031. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-481-4_22
Download citation
DOI: https://doi.org/10.1007/978-1-62703-481-4_22
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-480-7
Online ISBN: 978-1-62703-481-4
eBook Packages: Springer Protocols