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Immunolocalization of Meiotic Proteins in Brassicaceae: Method 1

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Book cover Plant Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 990))

Abstract

Plant meiosis studies have enjoyed a fantastic boom in recent years with the use of Arabidopsis thaliana as an important model species for developmental studies because of its small genome, short life cycle, and large mutant collections. Unlike other eukaryotic models, plant meiosis does not display strict checkpoints and rarely commits to apoptotic processes, which makes it possible to investigate the whole meiotic process (spanning from premeiotic interphase to spore formation) in knockout mutants. In this chapter we describe a protocol for immunolabelling Arabidopsis and Brassica meiotic proteins on robustly spread chromosomes. This protocol allows the detection of a large range of proteins on well-preserved chromosomes and throughout the entire meiotic process.

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References

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Acknowledgments

We thank Wayne Crismani for constructive reading of the manuscript, and C. Franklin and C. Makaroff for providing ASY1 and REC8 antibodies, respectively.

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Authors and Affiliations

  1. Institut National de la Recherche Agronomique (INRA), Centre de Versailles-Grignon, Versailles Cedex, France

    Liudmila A. Chelysheva, Laurie Grandont & Mathilde Grelon

Authors
  1. Liudmila A. Chelysheva
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  2. Laurie Grandont
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  3. Mathilde Grelon
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Editor information

Editors and Affiliations

  1. , Dept. of Plant Breeding & Genetics, Cornell University, Bradfield Hall 401, Ithaca, 14853, New York, USA

    Wojciech P. Pawlowski

  2. , Centre de Versailles-Grignon, Inst National de la Recherche Agronomiqu, Route de St-Cyr (RD10), Versailles, F-78000, France

    Mathilde Grelon

  3. School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom

    Susan Armstrong

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© 2013 Springer Science+Business Media New York

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Chelysheva, L.A., Grandont, L., Grelon, M. (2013). Immunolocalization of Meiotic Proteins in Brassicaceae: Method 1. In: Pawlowski, W., Grelon, M., Armstrong, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 990. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-333-6_9

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  • DOI: https://doi.org/10.1007/978-1-62703-333-6_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-332-9

  • Online ISBN: 978-1-62703-333-6

  • eBook Packages: Springer Protocols

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